2. Cell Cycle/DNA Damage Epigenetics Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Sirtuin PPAR Fatty Acid Synthase (FASN) c-Myc Bacterial
  4. Agrimol B

Agrimol B, a polyphenol, is an orally active and potent SIRT1 activator. Agrimol B shows anti-adipogenic and anticancer activity. Agrimol B shows antibacterial activity against plant pathogens. Agrimol B dramatically inhibits 3T3-L1 adipocyte differentiation by reducing PPARγ, C/EBPα, FAS, UCP-1, and apoE expression. The action of Agrimol B on the cancer cells is likely derived from its effect on c-MYC, SKP2 and p27.

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CAS 番号 : 55576-66-4

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10 mg $260 在庫あり
25 mg $520 在庫あり
50 mg $850 在庫あり
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カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Agrimol B 関連抗体

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
IF
WB

    Agrimol B purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jul 24:116453.  [Abstract]

    Cytotoxicity was texted by CKK8 assay treated with Agrimol B (20, 50, 100 μM).

    Agrimol B purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jul 24:116453.  [Abstract]

    JC-1 fluorescent staining of PSCs after 3 days treated with 50 µM, 100 µM of Agrimol B.

    Agrimol B purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jul 24:116453.  [Abstract]

    Western blotting showed LC3, p62 activation in PSCs cells treated with Agrimol B (25, 50, 100 μM) in a time-dose dependent manner.

    Sirtuin アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7 SIRT4

    PPAR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    PPARα PPARβ/δ PPARγ PPAR PPARδ

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Agrimol B, a polyphenol, is an orally active and potent SIRT1 activator. Agrimol B shows anti-adipogenic and anticancer activity. Agrimol B shows antibacterial activity against plant pathogens. Agrimol B dramatically inhibits 3T3-L1 adipocyte differentiation by reducing PPARγ, C/EBPα, FAS, UCP-1, and apoE expression. The action of Agrimol B on the cancer cells is likely derived from its effect on c-MYC, SKP2 and p27[1][2][3].

    IC50 & Target[1]

    SIRT1

     

    PPARγ

     

    体外実験

    Agrimol B blocks adipogenesis at the early stage of differentiation in a dose-dependent manner, with an IC50 of 3.35 ± 0.32 μM[1].
    ? Agrimol B induces SIRT1 (silent information regulator 2 homolog 1) translocation and expression in 3T3-L1 adipocytes[1].
    ? Agrimol B inhibits PC-3 and A549 cells growth, with GI50 (growth inhibition 50%) values of 29 and 19 μM, GI75 values of 49 and 50 μM, and GI90 values of 63 and 76 μM, respectively[2].
    ? Agrimol B (0-76 μM) dose-dependently increases cells at G0 in both cell lines[2].
    ? Agrimol B (0-76 μM, 3 days) reduces the protein expression of c-MYC and SKP2 (S-phase kinase-associated protein 2), increases p27 (cyclin-dependent kinase inhibitor 1B), and down-regulates SPT16 (Suppressor of Ty Homolog-16) and SSRP1 (Structure-Specific Recognition Protein 1)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Agrimol B 関連抗体

    Cell Cycle Analysis[2]

    Cell Line: PC-3 and A549 cells
    Concentration: 29 and 63 μM (PC-3), 19 and 76 μM (A549)
    Incubation Time: 10 min
    Result: Dose-dependently increased cells at G0 in both cell lines. Reduced percentage of cells positive for Ki-67, and increased p27 positive cell population in PC-3 and A549 cells.

    Western Blot Analysis[2]

    Cell Line: PC-3 and A549 cells
    Concentration: 0, 29, 49, 63 μM (PC-3); 0, 19, 50, and 76 μM (A549)
    Incubation Time: 3 days
    Result: Reduced c-MYC, SKP2 and increased p27 in both cell lines, and down-regulated SPT16 and SSRP1 in A549 cells with no effect on CRM1 in both cell lines.

    Immunofluorescence[1]

    Cell Line: 3T3-L1 preadipocyte
    Concentration: 0, 3, and 10 μM
    Incubation Time: 6 days
    Result: Significantly increased nuclear positive rate of SIRT1; Markedly increased SIRT1 expression at 10 μM, the effect vanished at 3 μM. Down-regulated PPARγ and C/EBPα (CCAAT/enhancer-binding protein α) expression; Significantly decreased FAS (fatty acid synthesis), UCP-1 (uncoupling protein-1), and apoE (apolipoprotein E) expression at 10 μM.
    体内実験

    Agrimol B (10 mg/kg, Orally, daily) reduces growth of prostate cancer cell xenograft in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c nude mice (6-week-old, male, injected with PC-3 cells in 0.2 mL PBS subcutaneously)[2]
    Dosage: 10 mg/kg
    Administration: Orally, daily, after 15 days of cancer cell implantation
    Result: Inhibited tumor growth in a mouse model of human prostate cancer, reduced the tumor volume at day 31 and day 32.
    分子量

    682.75

    分子式

    C37H46O12
    CAS 番号
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    CC[C@H](C)C(C1=C(O)C(CC2=C(O)C(C(CCC)=O)=C(OC)C(C)=C2O)=C(O)C(CC3=C(O)C(C(CCC)=O)=C(OC)C(C)=C3O)=C1O)=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 5 mg/mL (7.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4647 mL 7.3233 mL 14.6467 mL
    5 mM 0.2929 mL 1.4647 mL 2.9293 mL
    10 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.5 mg/mL (0.73 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 0.5 mg/mL (0.73 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

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    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.75%

    COA (255 KB) HNMR (303 KB) RP-HPLC (255 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.4647 mL 7.3233 mL 14.6466 mL 36.6166 mL
    5 mM 0.2929 mL 1.4647 mL 2.9293 mL 7.3233 mL
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    Agrimol B Related Classifications

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Agrimol B55576-66-4SirtuinPPARFatty Acid Synthase (FASN)c-MycBacterialPeroxisome proliferator-activated receptorsMycAgrimonia pilosa Ledeb.Lipid dropletObesityPPARγSIRT1Anti-adipogenicAntibacterialQuiescent cancer cellsProstate cancerLung cancerPolyphenolsadipogenesisInhibitorinhibitorinhibit

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