H-8 dihydrochloride

Name: H-8 dihydrochloride
Brand: MedChemExpress
SKU: HY-112465
Rating: 4.5 (0 reviews)

Cat. No.: HY-112465 Purity: 99.19%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

H-8 dihydrochloride is a selective Cyclic nucleotide-dependent protein kinase inhibitor. H-8 dihydrochloride potently inhibits cGMP- and cAMP-dependent protein phosphorylation. H-8 dihydrochloride enhances the vasodilatory effect induced by 8-bromo-cAMP (HY-12306A). H-8 dihydrochloride fails to attenuate the vasodilatory effects induced by 8-bromo-cGMP (HY-101379A), atrial natriuretic peptide II, or Sodium nitroprusside (HY-B0564) in rat aorta.

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H-8 dihydrochloride 화학구조

CAS No. : 113276-94-1

사이즈	재고	수량
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of H-8 dihydrochloride:

Protein kinase inhibitor 7 견적 받기

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKA Isoform Specific Products:

View All Isoforms

PKA PKAR PKACα

View All PKC Isoform Specific Products:

View All Isoforms

PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

H-8 dihydrochloride (1-5 μM; 10 min preincubation) potently inhibits cGMP- and cAMP-dependent protein phosphorylation in intestinal homogenates of winter flounder, but does not inhibit Ca²⁺-dependent protein phosphorylation, with the 5 μM concentration achieving nearly complete inhibition of cyclic nucleotide-dependent targets^[1].
H-8 (5-100 μM) dihydrochloride significantly stimulates amiloride-sensitive Na⁺ transport in primary cultured rat fetal lung type II alveolar epithelial cells at a concentration of 100 μM, whereas no significant effect on this transport is observed at 5 μM, and it does not inhibit terbutaline-induced activation of this transport^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

338.25

화학식

C₁₂H₁₇Cl₂N₃O₂S

CAS No.

113276-94-1

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC)=O.Cl.Cl

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도

In Vitro:

DMSO : 20.83 mg/mL (61.58 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9564 mL	14.7820 mL	29.5639 mL
5 mM	0.5913 mL	2.9564 mL	5.9128 mL
10 mM	0.2956 mL	1.4782 mL	2.9564 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.19%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (253 KB) HNMR (265 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. O'Grady SM, et al. Cyclic nucleotide-dependent protein kinase inhibition by H-8: effects on ion transport. Am J Physiol. 1988;254(1 Pt 1):C115-C121. [Content Brief]

[2]. Daugirdas JT, et al. Effect of H-8, an isoquinolinesulfonamide inhibitor of cyclic nucleotide-dependent protein kinase, on cAMP- and cGMP-mediated vasorelaxation. Blood Vessels. 1991;28(5):366-371.

[3]. Niisato N, et al. Effects of PKA inhibitors, H-compounds, on epithelial Na+ channels via PKA-independent mechanisms. Life Sci. 1999;65(10):PL109-PL114. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9564 mL	14.7820 mL	29.5639 mL	73.9098 mL
	5 mM	0.5913 mL	2.9564 mL	5.9128 mL	14.7820 mL
	10 mM	0.2956 mL	1.4782 mL	2.9564 mL	7.3910 mL
	15 mM	0.1971 mL	0.9855 mL	1.9709 mL	4.9273 mL
	20 mM	0.1478 mL	0.7391 mL	1.4782 mL	3.6955 mL
	25 mM	0.1183 mL	0.5913 mL	1.1826 mL	2.9564 mL
	30 mM	0.0985 mL	0.4927 mL	0.9855 mL	2.4637 mL
	40 mM	0.0739 mL	0.3695 mL	0.7391 mL	1.8477 mL
	50 mM	0.0591 mL	0.2956 mL	0.5913 mL	1.4782 mL
	60 mM	0.0493 mL	0.2464 mL	0.4927 mL	1.2318 mL