8-Bromo-cGMP sodium

Name: 8-Bromo-cGMP sodium
Brand: MedChemExpress
SKU: HY-101379A
Rating: 5.0 (6 reviews)

Cat. No.: HY-101379A Purity: 99.89%: Data Sheet
COA

SDS
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8-Bromo-cGMP sodium, a membrane-permeable analogue of cGMP, is a PKG (protein kinase G) activator. 8-Bromo-cGMP sodium significantly inhibits Ca²⁺ macroscopic currents and impairs insulin release stimulated with high K⁺. 8-Bromo-cGMP sodium has antinociceptive effects and results in vasodilator responses.

For research use only. We do not sell to patients.

CAS No. : 51116-01-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of 8-Bromo-cGMP sodium:

8-Bromo-cGMP sodium (Standard) Get quote

6 Publications Citing Use of MCE 8-Bromo-cGMP sodium

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Cell Imaging/Staining

: 8-Bromo-cGMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 23;302(3):111202. [Abstract]; Whole-cell recording shows the effect of ET-1 on NPPB-sensitive Cl^- currents in DCT1 in the presence of 1 mM 8-Bromo-cGMP sodium (cGMP analog, blue line), using a ramp protocol. The black line represents the typical NPPB-sensitive Cl^- currents. The red line depicts the effect of 8-Bromo-cGMP sodium on the Cl^- current. The drug was introduced into the bath 5 to 7 min prior.

: 8-Bromo-cGMP sodium purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 24:e16181. [Abstract]; Functional validation of PKG modulation. Control: multinucleated TRAP (+) cells; 8-Bromo-cGMP sodium (0.1-0.2 mM): reduced OCs formation; KT5823 (PKG inhibitor): Enhanced OCs maturation.

: 8-Bromo-cGMP sodium purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 24:e16181. [Abstract]; Immunofluorescence of PKG signaling components: Increased expression of: PKG (red) and VASP (red). Cytoskeleton counterstained with FITC (green); Nuclei counterstained with DAPI (blue). 1: Normal control, 2: AP treatment, 3: 8-Bromo-cGMP sodium treatment, 4: KT5823 treatment.

: 8-Bromo-cGMP sodium purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 31:245:283-300. [Abstract]; Cell viability of CCCP-R HK2 cells with rhBNP or 8-Bromo-cGMP sodium (10 μM, pretreatment for 30 minutes) treatment. n = 8 each group.

: 8-Bromo-cGMP sodium purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 31:245:283-300. [Abstract]; The activity of RhoA measured by G-LISA assay in CCCP-R HK2 cells treated with rhBNP or 8-Bromo-cGMP sodium (10 μM, pretreatment for 30 minutes) treatment.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

8-Bromo-cGMP sodium, a membrane-permeable analogue of cGMP, is a PKG (protein kinase G) activator. 8-Bromo-cGMP sodium significantly inhibits Ca²⁺ macroscopic currents and impairs insulin release stimulated with high K⁺^[1]. 8-Bromo-cGMP sodium has antinociceptive effects and results in vasodilator responses^[2].

In Vitro

8-Bromo-cGMP sodium (1-100 μM; 8 h) increases resistance of LLC-PK1 cells to CsA toxicity concentration-dependently^[3].
8-Bromo-cGMP sodium (1-100 μM; 16 h) induces the synthesis of HO-1 protein in a concentration-dependent fashion^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	LLC-PK1 cells (ATCC CL 101)
Concentration:	1-100 μM
Incubation Time:	8 hours
Result:	Increased resistance of LLC-PK1 cells to Cyclosporin A (CsA) toxicity concentration-dependently and augmented cell viability by up to 65%.

Western Blot Analysis^[3]

Cell Line:	LLC-PK1 cells (ATCC CL 101)
Concentration:	1-100 μM
Incubation Time:	16 hours
Result:	Induced the synthesis of HO-1 protein in a concentration-dependent fashion.

In Vivo

8-Bromo-cGMP sodium (0.3, 1, 3.0 nmol; intrathecal administration; 10 min before test) dose-dependently and significantly increases the tail-flick latency in Vincristine-treated mice to the level observed in vehicle-treated naive mice (male ICR mice, 4weeks of age and weighing 20 g). Vincristine (0.05 mg/kg 1 day after the pre-drug tail-flick latency, and then 0.125 mg/kg twice a week for 6 weeks) can induce painful neuropathy in mice^[4].
8-Bromo-cGMP sodium (10 mg/kg; iv; single dose) results in vasodilator responses in eNOS-Tg mice and WT littermates in C57BL/6 background (19-35 g)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

446.08

Formula

C₁₀H₁₀BrN₅NaO₇P

CAS No.

51116-01-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C2=C(N([C@H]3[C@H](O)[C@@](OP(OC4)(O[Na])=O)([H])[C@]4([H])O3)C(Br)=N2)NC(N)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (224.18 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2418 mL	11.2088 mL	22.4175 mL
5 mM	0.4484 mL	2.2418 mL	4.4835 mL
10 mM	0.2242 mL	1.1209 mL	2.2418 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (224.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (258 KB) HNMR (275 KB) RP-HPLC (239 KB) MS (204 KB)

Handling Instructions (2659 KB)

References

[1]. Sarkar O , et al. Nitric oxide attenuates overexpression of Giα proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling. PLoS One. 2017 Jul 10;12(7):e0179301. [Content Brief]

[2]. Tegeder I , et al. Dual effects of spinally delivered 8-bromo-cyclic guanosine mono-phosphate (8-bromo-cGMP) in formalin-induced nociception in rats. Neurosci Lett. 2002 Oct 31;332(2):146-50. [Content Brief]

[3]. Tobias Polte, et al. Atrial natriuretic peptide reduces cyclosporin toxicity in renal cells: role of cGMP and heme oxygenase-1. Free Radic Biol Med. 2002 Jan 1;32(1):56-63. [Content Brief]

[4]. Junzo Kamei, et al. Possible involvement of the spinal nitric oxide/cGMP pathway in vincristine-induced painful neuropathy in mice. Pain. 2005 Sep;117(1-2):112-20. [Content Brief]

[5]. Elza D van Deel, et al. Vasomotor control in mice overexpressing human endothelial nitric oxide synthase. Am J Physiol Heart Circ Physiol. 2007 Aug;293(2):H1144-53. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.2418 mL	11.2088 mL	22.4175 mL	56.0438 mL
	5 mM	0.4484 mL	2.2418 mL	4.4835 mL	11.2088 mL
	10 mM	0.2242 mL	1.1209 mL	2.2418 mL	5.6044 mL
	15 mM	0.1495 mL	0.7473 mL	1.4945 mL	3.7363 mL
	20 mM	0.1121 mL	0.5604 mL	1.1209 mL	2.8022 mL
	25 mM	0.0897 mL	0.4484 mL	0.8967 mL	2.2418 mL
	30 mM	0.0747 mL	0.3736 mL	0.7473 mL	1.8681 mL
	40 mM	0.0560 mL	0.2802 mL	0.5604 mL	1.4011 mL
	50 mM	0.0448 mL	0.2242 mL	0.4484 mL	1.1209 mL
	60 mM	0.0374 mL	0.1868 mL	0.3736 mL	0.9341 mL
	80 mM	0.0280 mL	0.1401 mL	0.2802 mL	0.7005 mL
	100 mM	0.0224 mL	0.1121 mL	0.2242 mL	0.5604 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.