UNC6852

Name: UNC6852
Brand: MedChemExpress
SKU: HY-130708
Rating: 5.0 (4 reviews)

Art. -Nr.: HY-130708 Reinheit: 98.49%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

UNC6852 is a selective polycomb repressive complex 2 (PRC2) degrader based on PROTAC and contains an EED (embryonic ectoderm development) ligand and a von Hippel-Lindau ligand, with an IC₅₀ of 247 nM for EED.
(Pink: PRC2 ligand (HY-130979); Blue: VHL ligand (HY-125845); Black: linker (HY-N0067)).

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CAS. Nr. : 2688842-08-0

Größe	Verfügbarkeit	Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	Auf Lager	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Auf Lager	Estimated Time of Arrival: December 31
Solid
1 mg	Auf Lager	Estimated Time of Arrival: December 31
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
50 mg	Angebot einholen
100 mg	Angebot einholen

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This product is a controlled substance and not for sale in your territory.

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Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE UNC6852

In Vivo Efficacy Study

Bio/Physico-chemical Assay

RT-PCR

: UNC6852 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Sep 18:2025.09.16.676613. [Abstract]; NLuc reporter activity measurements following 72-hour treatment with the indicated SAM-competitive EZH inhibitors or other PRC2 inhibitors, including EED inhibitors and EZH2 or EED PROTACs (UNC6852) (5-10 μM; 72 h).

: UNC6852 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2021 Jul 27;11(1):15238. [Abstract]; Comparison of time to occlusion of the caudal vein after laser injury between wild-type control 5 dpf larvae and UNC6852-injected larvae by one-way ANOVA. The x-axis represents four different concentrations of the UNC6852 (0.075 mM, 0.15 mM, 0.3 mM and 0.6 mM) (2 h) used for larval injections. The dot plots represent wild-type larvae (closed circles) and the knockdown larvae (closed triangles).

: UNC6852 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2021 Jul 27;11(1):15238. [Abstract]; Quantitative real-time PCR showing the fold change of tfpia gene expression in liver and spleen for UNC6852 (6 h)-injected adult zebrafish with wild-type control by Student’ s t test. The concentration of UNC6852 used for adult injections (0.6 mM) is indicated beneath the bar graph.

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D ASH1L/KMT2H SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 NSD3/WHSC1L1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

Alle PROTACs Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

Biologische Aktivität
Reinheit & Dokumentation
Verweise
Kundenbewertung

Beschreibung

UNC6852 is a selective polycomb repressive complex 2 (PRC2) degrader based on PROTAC and contains an EED (embryonic ectoderm development) ligand and a von Hippel-Lindau ligand, with an IC₅₀ of 247 nM for EED^[1]. (Pink: PRC2 ligand (HY-130979); Blue: VHL ligand (HY-125845); Black: linker (HY-N0067)).

IC₅₀ & Target

IC50: 247 nM (EED)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
DB	EC₅₀	3.4 μM Compound: 71; UNC6852	Antiproliferative activity against human DB cells assessed as viable cells with replenishment of medium with compound for every three days upto 9 days by trypan blue staining based automated bio-rad TC20 cell counter method Antiproliferative activity against human DB cells assessed as viable cells with replenishment of medium with compound for every three days upto 9 days by trypan blue staining based automated bio-rad TC20 cell counter method	[PMID: 35093670]
DB	EC₅₀	3.4 μM Compound: 71; UNC6852	Antiproliferative activity against human DB cells measured after 9 days by tryphan blue staining Antiproliferative activity against human DB cells measured after 9 days by tryphan blue staining	[PMID: 35093670]
Pfeiffer	EC₅₀	0.41 μM Compound: 71; UNC6852	Antiproliferative activity against human Pfeiffer cells assessed as viable cells with replenishment of medium with compound for every three days upto 6 days by trypan blue staining based automated bio-rad TC20 cell counter method Antiproliferative activity against human Pfeiffer cells assessed as viable cells with replenishment of medium with compound for every three days upto 6 days by trypan blue staining based automated bio-rad TC20 cell counter method	[PMID: 35093670]
Pfeiffer	IC₅₀	0.41 μM Compound: 71; UNC6852	Antiproliferative activity against human Pfeiffer cells measured after 3 days by tryphan blue staining Antiproliferative activity against human Pfeiffer cells measured after 3 days by tryphan blue staining	[PMID: 35093670]

In Vitro

UNC6852 mediates PRC2 degradation^[1].
UNC6852 displays no cellular toxicity at concentrations up to 30 μM for HeLa Cells^[1].
UNC6852 (10 μM; 1-72 hours) results in a decrease in the levels of both EED and EZH2^[1].
UNC6852 facilitates PRC2 degradation via VHL recruitment^[1].
UNC6852 selectively degrades EED and EZH2^[1].
UNC6852 reduces H3K27me3 levels and DLBCL cell proliferation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	DLBCL Cells
Concentration:	3 μM
Incubation Time:	0 -10 days
Result:	Exhibited anti-proliferative effects.

Western Blot Analysis^[1]

Cell Line:	HeLa Cells
Concentration:	10 μM
Incubation Time:	1 hours, 4 hours, 8 hours, 10 hours, 16 hours, 20 hours, 24 hours, 48 hours, 72 hours
Result:	Resulted in a decrease in the levels of both EED and EZH2.

Molekulargewicht

832.97

Formel

C₄₃H₄₈N₁₀O₆S

CAS. Nr.

2688842-08-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC=C(C=C1)C2=CN=C(N3C2=NN=C3)NCC4=CC=CO4)NCCCC(N[C@H](C(N5[C@@H](C[C@H](C5)O)C(NCC6=CC=C(C=C6)C7=C(N=CS7)C)=O)=O)C(C)(C)C)=O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (120.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2005 mL	6.0026 mL	12.0052 mL
5 mM	0.2401 mL	1.2005 mL	2.4010 mL
10 mM	0.1201 mL	0.6003 mL	1.2005 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (6.00 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (6.00 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (6.00 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: 98.49%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (249 KB) HNMR (211 KB) RP-HPLC (259 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Potjewyd F, et al. Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader. Cell Chem Biol. 2020 Jan 16;27(1):47-56.e15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2005 mL	6.0026 mL	12.0052 mL	30.0131 mL
	5 mM	0.2401 mL	1.2005 mL	2.4010 mL	6.0026 mL
	10 mM	0.1201 mL	0.6003 mL	1.2005 mL	3.0013 mL
	15 mM	0.0800 mL	0.4002 mL	0.8003 mL	2.0009 mL
	20 mM	0.0600 mL	0.3001 mL	0.6003 mL	1.5007 mL
	25 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2005 mL
	30 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0004 mL
	40 mM	0.0300 mL	0.1501 mL	0.3001 mL	0.7503 mL
	50 mM	0.0240 mL	0.1201 mL	0.2401 mL	0.6003 mL
	60 mM	0.0200 mL	0.1000 mL	0.2001 mL	0.5002 mL
	80 mM	0.0150 mL	0.0750 mL	0.1501 mL	0.3752 mL
	100 mM	0.0120 mL	0.0600 mL	0.1201 mL	0.3001 mL

UNC6852 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

UNC68522688842-08-0UNC 6852UNC-6852Histone MethyltransferasePROTACsPRC2EEDPROTACInhibitorinhibitorinhibit

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UNC6852

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UNC6852 Related Antibodies

4 Publications Citing Use of MCE UNC6852

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen:

Alle PROTACs Isoform-spezifische Produkte anzeigen:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

UNC6852 Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

UNC6852 Related Classifications

Keywords:

Ihre kürzlich angesehenen Produkte:

Kundenbewertung

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SAFETY DATA SHEET (SDS)

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