1. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis Anti-infection Cell Cycle/DNA Damage
  2. Reactive Oxygen Species (ROS) Endogenous Metabolite Apoptosis Ferroptosis Influenza Virus Disulfidptosis
  3. Acetylcysteine

N-Acetylcysteine  (Synonyms: NAC L; NAC; N-アセチル-L-システイン; アセチルシステイン; LNAC)

製品番号: HY-B0215 純度: 99.86%
COA 取扱説明書 Technical Support

Acetylcysteine (N-Acetylcysteine) is a mucolytic agent that can cross the blood-brain barrier, which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor. Acetylcysteine is a cysteine precursor, prevents hemin-induced ferroptosis by neutralizing toxic lipids generated by arachidonate-dependent activity of 5-lipoxygenases. Acetylcysteine induces cell apoptosis. Acetylcysteine also has anti-influenza virus activities. In addition, Acetylcysteine ​​is the most stable form of cysteine ​​during drug delivery and can be used in disulfidptosis studies.

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CAS 番号 : 616-91-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
500 mg $30 在庫あり
5 g $40 在庫あり
10 g $50 在庫あり
25 g $80 在庫あり
50 g $100 在庫あり
100 g $130 在庫あり
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カスタマーレビュー

Based on 661 publication(s) in Google Scholar

Other Forms of Acetylcysteine:

Top Publications Citing Use of Products

顧客検証

Cell Proliferation/Viability Assay
WB

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 7:e09208.  [Abstract]

    Trolox (100 µM), a hydrophilic analogue of VE with potent antioxidant effect, was used as a control of VE. After a short incubation time of 6 h, cathepsin inhibitors (CA‐074, Pep‐A, and E‐64), Ca2+ chelator, and cellular ROS scavengers (NAC, VC, and Trolox) displayed a delayed effect of ipLNP cytotoxicity.

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Sci Bull. 2024 Dec 15;69(23):3700-3716.

    Summary of cell viability measured for MCD-induced wild-type hepatocytes, hepatocytes treated with 2 mmol/L fatty acid (FA) for 24 h with the indicated concentrations of PAG together with 2 mmol/L N-acetylcysteine (NAC) or 5 μmol/L Fer-1.

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2024 Dec:197:107046.  [Abstract]

    BMDMs pretreated with degarelix (1 μM), aminoguanidine hydrochloride (AGHS, 100 μM), BafA1 (100 nM), NAC (5 mM), Z-VAD (20 μM), or 3-MA (5 mM) were infected with H37Rv (MOI = 2). At different time points after infection, bacterial survival was calculated by dividing the CFU at 24 h by the CFU at 3 h.

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2023 May 14.

    The viability of MC38 cells treated with PAD@MS for 48 h in the presence or absence of 10 µM Ferr-1 (Ferrostatin-1), 20 µM Nec-1 (Necrostatin-1), 10 µM Z-VAD-FMK, and 1 mM N-Aetyl-L-cysteine (NAC).

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2018 Apr 27:4:47.  [Abstract]

    Wild-type, Prx1KO, and Prx1OE cells are each divided into three groups, which are control groups, groups treated with 100 ng/mL LPS, and groups treated with 100 ng/mL LPS and NAC. Western blot analysis is then performed with antibodies against Bax, Bcl-2, Cyto-c, caspase 3, and GAPDH, and band intensity is measured with densitometry.

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Fundam Clin Pharmacol. 2017 Feb;31(1):64-74.  [Abstract]

    Advanced oxidation production product (AOPP)-triggered apoptosis-related signals are triggered by ROS production. Human chondrocytes are pretreated with N-Acetylcysteine (NAC; 5 mM) for 60 min, and then, chondrocyte are co-incubated with AOPP and NAC for 24 h. (a) Pretreatment of ROS scavenger NAC markedly alleviates AOPP-triggered mitochondria-mediated apoptosis proteins. (b) Pretreatment of chondrocyte with NAC abrogates the intracellular Ca2+ mobilization. (c) NAC pretreatment atte

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Sci Rep. 2016 Oct 27;6:35995.  [Abstract]

    Pretreatment of NAC, a free radical scavenger, largely decreases the expression of caspase-3 and almost abolishes the H2O2 induced upregulation of PRX1. Western blot analysis shows the pretreatment of osteoblast cells with E2 partially abrogates the increased PRX1 and caspase-3 expressions induced by H2O2.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

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    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    Acetylcysteine (N-Acetylcysteine) is a mucolytic agent that can cross the blood-brain barrier, which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor. Acetylcysteine is a cysteine precursor, prevents hemin-induced ferroptosis by neutralizing toxic lipids generated by arachidonate-dependent activity of 5-lipoxygenases. Acetylcysteine induces cell apoptosis. Acetylcysteine also has anti-influenza virus activities. In addition, Acetylcysteine ​​is the most stable form of cysteine ​​during drug delivery and can be used in disulfidptosis studies[1][2][3][4][5][6][7][8].

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    Hepatocyte EC50
    105.6 3
    Compound: N-acetyl-L-cysteine
    Hepatoprotective activity against H2O2-induced cell damage in rat hepatocyte assessed as reduction in cell growth
    Hepatoprotective activity against H2O2-induced cell damage in rat hepatocyte assessed as reduction in cell growth
    [PMID: 29353722]
    HT-22 EC50
    0.7 2
    Compound: N-Acetylcysteine
    Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
    Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
    [PMID: 32991171]
    HUVEC IC50
    10 2
    Compound: N-acetyl cysteine
    Antiinflammatory activity in HUVEC assessed as inhibition of TNFalpha-induced ICAM1 expression preincubated for 0.5 to 6 hrs followed by TNFalpha challenge measured after 4 hrs by immunostaining assay
    Antiinflammatory activity in HUVEC assessed as inhibition of TNFalpha-induced ICAM1 expression preincubated for 0.5 to 6 hrs followed by TNFalpha challenge measured after 4 hrs by immunostaining assay
    10.1039/C0MD00262C
    KB 3-1 IC50
    29.9 2
    Compound: NAC
    Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
    Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
    [PMID: 21657271]
    KB-V1 IC50
    28.8 2
    Compound: NAC
    Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
    Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
    [PMID: 21657271]
    SH-SY5Y EC50
    1.68 2
    Compound: NAC
    Neuroprotection against H2O2-induced cell death in human SH-SY5Y cells preincubated for 6 hrs followed by H2O2 addition and measured after 24 hrs by MTT assay
    Neuroprotection against H2O2-induced cell death in human SH-SY5Y cells preincubated for 6 hrs followed by H2O2 addition and measured after 24 hrs by MTT assay
    [PMID: 30470490]
    HT-22 EC50
    0.7 2
    Compound: N-Acetylcysteine
    Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
    Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
    [PMID: 32991171]
    HUVEC IC50
    10 2
    Compound: N-acetyl cysteine
    Antiinflammatory activity in HUVEC assessed as inhibition of TNFalpha-induced ICAM1 expression preincubated for 0.5 to 6 hrs followed by TNFalpha challenge measured after 4 hrs by immunostaining assay
    Antiinflammatory activity in HUVEC assessed as inhibition of TNFalpha-induced ICAM1 expression preincubated for 0.5 to 6 hrs followed by TNFalpha challenge measured after 4 hrs by immunostaining assay
    10.1039/C0MD00262C
    Hepatocyte EC50
    105.6 3
    Compound: N-acetyl-L-cysteine
    Hepatoprotective activity against H2O2-induced cell damage in rat hepatocyte assessed as reduction in cell growth
    Hepatoprotective activity against H2O2-induced cell damage in rat hepatocyte assessed as reduction in cell growth
    [PMID: 29353722]
    KB 3-1 IC50
    29.9 2
    Compound: NAC
    Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
    Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
    [PMID: 21657271]
    KB-V1 IC50
    28.8 2
    Compound: NAC
    Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
    Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
    [PMID: 21657271]
    SH-SY5Y EC50
    1.68 2
    Compound: NAC
    Neuroprotection against H2O2-induced cell death in human SH-SY5Y cells preincubated for 6 hrs followed by H2O2 addition and measured after 24 hrs by MTT assay
    Neuroprotection against H2O2-induced cell death in human SH-SY5Y cells preincubated for 6 hrs followed by H2O2 addition and measured after 24 hrs by MTT assay
    [PMID: 30470490]
    体外実験

    Acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. Acetylcysteine also prevents death of PC12 cells and sympathetic neurons[2].
    Acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells[3].
    Acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. Acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. Acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. Acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that Acetylcysteine interferes with redox-sensitive steps in the NGF mechanism[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    体内実験

    Acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group Acetylcysteine 300 mg/kg. Lung glutathione peroxidase is significantly increases in group Acetylcysteine 300 mg/kg(P=0.04), while the other oxidation biomarkers show no significant differences[6].
    Acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    臨床実験
    分子量

    163.20

    分子式

    C5H9NO3S

    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    SC[C@@H](C(O)=O)NC(C)=O

    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (612.75 mM; Need ultrasonic)

    DMSO : 100 mg/mL (612.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.1275 mL 30.6373 mL 61.2745 mL
    5 mM 1.2255 mL 6.1275 mL 12.2549 mL
    10 mM 0.6127 mL 3.0637 mL 6.1275 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます:C1V1 = C2V2

    濃度 (開始)

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    ×
    体積 (開始)

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (15.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (15.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 120 mg/mL (735.29 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.86%

    参考文献
    細胞実験
    [2]

    For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving "primed" PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [6]

    Rats: Rats are randomLy allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given Acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group Acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group Acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group Acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 6.1275 mL 30.6373 mL 61.2745 mL 153.1863 mL
    5 mM 1.2255 mL 6.1275 mL 12.2549 mL 30.6373 mL
    10 mM 0.6127 mL 3.0637 mL 6.1275 mL 15.3186 mL
    15 mM 0.4085 mL 2.0425 mL 4.0850 mL 10.2124 mL
    20 mM 0.3064 mL 1.5319 mL 3.0637 mL 7.6593 mL
    25 mM 0.2451 mL 1.2255 mL 2.4510 mL 6.1275 mL
    30 mM 0.2042 mL 1.0212 mL 2.0425 mL 5.1062 mL
    40 mM 0.1532 mL 0.7659 mL 1.5319 mL 3.8297 mL
    50 mM 0.1225 mL 0.6127 mL 1.2255 mL 3.0637 mL
    60 mM 0.1021 mL 0.5106 mL 1.0212 mL 2.5531 mL
    80 mM 0.0766 mL 0.3830 mL 0.7659 mL 1.9148 mL
    100 mM 0.0613 mL 0.3064 mL 0.6127 mL 1.5319 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます:C1V1 = C2V2

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    × = ×
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    製品名:
    Acetylcysteine
    製品番号:
    HY-B0215
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