Rutin (Synonyms: Rutoside; Quercetin 3-O-rutinoside)

Name: Rutin
Brand: MedChemExpress
SKU: HY-N0148
Rating: 5.0 (30 reviews)

Cat. No.: HY-N0148 Purity: 98.09%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Rutin (Rutoside) is a flavonoid found in many plants and shows a wide range of biological activities including anti-inflammatory, antidiabetic, antioxidant, neuroprotective, nephroprotective, hepatoprotective and reducing Aβ oligomer activities. Rutin is also a CBR1 inhibitor, which can cross the blood brain barrier. Rutin attenuates vancomycin-induced renal tubular cell apoptosis via suppression of apoptosis, mitochondrial dysfunction, and oxidative stress.

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CAS No. : 153-18-4

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
500 mg	해외재고보유	Estimated Time of Arrival: December 31
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 30 publication(s) in Google Scholar

Other Forms of Rutin:

Rutin hydrate In-stock
Rutin trihydrate In-stock
Rutin (Standard) In-stock

30 Publications Citing Use of MCE Rutin

Histological Imaging/Staining

ELISA

Cell Proliferation/Viability Assay

: Rutin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Rutin (80 mg/kg, q.d., 3 days) reduces pathological damage. Scale bar = 50 μm, n = 6 per group.

: Rutin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. (A-H) Western blot analysis of the protein expression level of NF-κB p65, NLRP3, Caspase-1, GSDMD in MCAO/R rats brain tissues and grayscale scanning analysis. Scale bar = 20 μm. n = 3 per group.

: Rutin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. ELISA analysis of IL-1β, IL-18, IL-6, TNF-α levels in serum. Scale bar = 20 μm. n = 3 per group.

: Rutin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. Representative immunofluorescence staining pictures of NLRP3 in brain tissues. Scale bar = 20 μm. n = 3 per group.

: Rutin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (40 μM, 24 h) on OGD/R-induced primary astrocyte during reperfusion. Representative immunofluorescence staining pictures of NLRP3, GSDMD in OGD/R-induced primary astrocyte. Scale bar = 20 μm, n = 3 per group.

: Rutin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (5, 10, 20, and 40 μM, 24 h) on OGD/R-induced primary astrocyte. Cell viability was determined with CCK-8 kit.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

CBR1^[5]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by SRB assay Cytotoxicity against human A549 cells after 48 hrs by SRB assay	[PMID: 28165740]
BALB/3T3	IC₅₀	> 164 μM Compound: 6	Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay	[PMID: 10096863]
BMDC	IC₅₀	> 50 μM Compound: 14	Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 14	Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 14	Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BT-549	IC₅₀	> 10 μM Compound: 12	Cytotoxicity against human BT549 cells after 48 hrs by SRB assay Cytotoxicity against human BT549 cells after 48 hrs by SRB assay	[PMID: 28165740]
BV-2	IC₅₀	24.29 μM Compound: 12	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay	[PMID: 28165740]
HEK293	EC₅₀	1.2 μM Compound: Rutin	Agonist activity at human NMU2R transfected in HEK293 cells after 7 hrs by luciferase reporter gene assay Agonist activity at human NMU2R transfected in HEK293 cells after 7 hrs by luciferase reporter gene assay	[PMID: 25262941]
K562	IC₅₀	897 μM Compound: Rutin, Quericitin-3-rutinoside, Sophorin	Induction of apoptosis in human K562 cells assessed as total apoptotic cells after 3 days by Annexin-V staining-based assay Induction of apoptosis in human K562 cells assessed as total apoptotic cells after 3 days by Annexin-V staining-based assay	[PMID: 25496805]
KB	ED₅₀	26 μg/mL Compound: NSC-115918	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
L929	EC₅₀	250 μM Compound: rutin	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining	[PMID: 9287415]
L929	EC₅₀	> 250 μM Compound: rutin	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay	[PMID: 9287415]
L929	IC₅₀	> 100 μM Compound: Rutin	Cytotoxicity against mouse L929 cells after 5 days by MTS assay Cytotoxicity against mouse L929 cells after 5 days by MTS assay	[PMID: 22743241]
MDCK	CC₅₀	> 328 μM Compound: Rutin	Cytotoxicity against MDCK cells Cytotoxicity against MDCK cells	[PMID: 25096296]
N9	IC₅₀	> 100 μM Compound: 12	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]
RAW264.7	IC₅₀	15.07 μM Compound: Rutin	Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of PMA-induced macrophages respiratory burst measured up to 30 min by chemiluminescence method Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of PMA-induced macrophages respiratory burst measured up to 30 min by chemiluminescence method	10.1007/s00044-013-0504-9
RAW264.7	IC₅₀	27.5 μM Compound: 18	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by griess reaction analysis Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by griess reaction analysis	[PMID: 24679441]
RAW264.7	IC₅₀	44.5 μM Compound: 18	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA	[PMID: 24679441]
RD	EC₅₀	109.63 μM Compound: Rutin	Antiviral activity against EV71 infected in human RD cells assessed as reduction in viral plaque formation after 1 hr by crystal violet staining method Antiviral activity against EV71 infected in human RD cells assessed as reduction in viral plaque formation after 1 hr by crystal violet staining method	[PMID: 27776325]
Raji	IC₅₀	578 molar ratio Compound: 15	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA	[PMID: 17190444]
SK-MEL-2	IC₅₀	> 10 μM Compound: 12	Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay	[PMID: 28165740]
SK-OV-3	IC₅₀	> 10 μM Compound: 12	Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay	[PMID: 28165740]

In Vivo

Rutin hydrate ameliorates cadmium chloride-induced spatial memory loss and neural apoptosis in rats by enhancing levels of acetylcholine, inhibiting JNK and ERK1/2 activation and activating mTOR signaling^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

610.52

화학식

C₂₇H₃₀O₁₆

CAS No.

153-18-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO[C@H]3[C@@H]([C@@H]([C@H]([C@H](C)O3)O)O)O)O2)O)O)O)=C(C4=CC=C(O)C(O)=C4)OC5=CC(O)=CC(O)=C15

Structure Classification

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 50 mg/mL (81.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6379 mL	8.1897 mL	16.3795 mL
5 mM	0.3276 mL	1.6379 mL	3.2759 mL
10 mM	0.1638 mL	0.8190 mL	1.6379 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 40 mg/mL (65.52 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 98.09%

Select Batch:

Data Sheet (284 KB) SDS (393 KB)

COA (263 KB) HNMR (206 KB) RP-HPLC (205 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Ghorbani A. Mechanisms of antidiabetic effects of flavonoid rutin. Biomed Pharmacother. 2017;96:305-312. [Content Brief]

[2]. Habtemariam S. Rutin as a Natural Therapy for Alzheimer's Disease: Insights into its Mechanisms of Action. Curr Med Chem. 2016;23(9):860-873. [Content Brief]

[3]. Xu PX, et al. Rutin improves spatial memory in Alzheimer's disease transgenic mice by reducing Aβ oligomer level and attenuating oxidative stress and neuroinflammation. Behav Brain Res. 2014;264:173-180. [Content Brief]

[4]. Abdel-Aleem GA, et al. Rutin hydrate ameliorates cadmium chloride-induced spatial memory loss and neural apoptosis in rats by enhancing levels of acetylcholine, inhibiting JNK and ERK1/2 activation and activating mTOR signalling. Arch Physiol Biochem. 2018;124(4):367-377. [Content Brief]

[5]. Carlquist M, et al. Flavonoids as inhibitors of human carbonyl reductase 1. Chem Biol Interact. 2008 Jul 30;174(2):98-108. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6379 mL	8.1897 mL	16.3795 mL	40.9487 mL
	5 mM	0.3276 mL	1.6379 mL	3.2759 mL	8.1897 mL
	10 mM	0.1638 mL	0.8190 mL	1.6379 mL	4.0949 mL
	15 mM	0.1092 mL	0.5460 mL	1.0920 mL	2.7299 mL
	20 mM	0.0819 mL	0.4095 mL	0.8190 mL	2.0474 mL
	25 mM	0.0655 mL	0.3276 mL	0.6552 mL	1.6379 mL
	30 mM	0.0546 mL	0.2730 mL	0.5460 mL	1.3650 mL
	40 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	50 mM	0.0328 mL	0.1638 mL	0.3276 mL	0.8190 mL
	60 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6825 mL
	80 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5119 mL

Rutin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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비고

Rutin (Synonyms: Rutoside; Quercetin 3-O-rutinoside)

고객리뷰

Other Forms of Rutin:

30 Publications Citing Use of MCE Rutin

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•Related Screening Libraries:

•Related Small Molecules:

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View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Rutin Related Classifications

Keywords:

최근 본 상품:

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Rutin (Synonyms: Rutoside; Quercetin 3-O-rutinoside)

고객리뷰

Other Forms of Rutin:

Rutin Related Antibodies

30 Publications Citing Use of MCE Rutin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Rutin Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report