Rutin trihydrate (Synonyms: Rutoside trihydrate; Quercetin 3-O-rutinoside trihydrate)

Name: Rutin trihydrate
Brand: MedChemExpress
SKU: HY-W013075
Rating: 5.0 (30 reviews)

Cat. No.: HY-W013075 Purity: 96.15%: Data Sheet
COA

SDS
Handling Instructions
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Rutin (Rutoside) trihydrate is a multifunctional natural flavonoid glycoside. Rutin trihydrate has been demonstrating excellent antioxidant, anti-inflammatory, anti-diabetic, and anti-carcinogenic properties. Cardioprotective and neuroprotective activities .

For research use only. We do not sell to patients.

CAS No. : 250249-75-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Based on 30 publication(s) in Google Scholar

Other Forms of Rutin trihydrate:

Rutin In-stock
Rutin hydrate In-stock

30 Publications Citing Use of MCE Rutin trihydrate

Histological Imaging/Staining

ELISA

Cell Proliferation/Viability Assay

: Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Rutin (80 mg/kg, q.d., 3 days) reduces pathological damage. Scale bar = 50 μm, n = 6 per group.

: Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. (A-H) Western blot analysis of the protein expression level of NF-κB p65, NLRP3, Caspase-1, GSDMD in MCAO/R rats brain tissues and grayscale scanning analysis. Scale bar = 20 μm. n = 3 per group.

: Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. ELISA analysis of IL-1β, IL-18, IL-6, TNF-α levels in serum. Scale bar = 20 μm. n = 3 per group.

: Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. Representative immunofluorescence staining pictures of NLRP3 in brain tissues. Scale bar = 20 μm. n = 3 per group.

: Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (40 μM, 24 h) on OGD/R-induced primary astrocyte during reperfusion. Representative immunofluorescence staining pictures of NLRP3, GSDMD in OGD/R-induced primary astrocyte. Scale bar = 20 μm, n = 3 per group.

: Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]; Effect of rutin (5, 10, 20, and 40 μM, 24 h) on OGD/R-induced primary astrocyte. Cell viability was determined with CCK-8 kit.

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Description

In Vitro

Rutin (Rutoside) trihydrate (0.5- 160 μM/ml; PC12 cells) decreases the acrylamide (ACR)-induced (5.46 mM) cytotoxicity significantly. ACR (5.46 mM) decreases cell viability in PC12 cells and pretreatment with rutin (0.5, 1, 1.5, 2.5, 5, 10, 20 and 40 μM/ml) increases the cell viability^[2].
Due to the ability of Rutin and/or its metabolites to cross the blood brain barrier, it has also been shown to modify the cognitive and various behavioral symptoms of neurodegenerative diseases^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pretreatment of Rutin (100 and 200 mg/kg/day; i.p.) for long-term caused behavioural and neurochemical changes in aged WAG male rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

664.56

Formula

C₂₇H₃₆O₁₉

CAS No.

250249-75-3

Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO[C@H]3[C@@H]([C@@H]([C@H]([C@H](C)O3)O)O)O)O2)O)O)O)=C(C4=CC=C(O)C(O)=C4)OC5=CC(O)=CC(O)=C15.O.O.O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (75.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5048 mL	7.5238 mL	15.0476 mL
5 mM	0.3010 mL	1.5048 mL	3.0095 mL
10 mM	0.1505 mL	0.7524 mL	1.5048 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (286 KB) SDS (393 KB)

COA (265 KB)

Handling Instructions (2659 KB)

References

[1]. Mel MMRD, et al. Formulation of microencapsulated rutin and evaluation of bioactivity and stability upon in vitro digestive and dialysis conditions. Int J Biol Macromol. 2020;159:316-323. [Content Brief]

[2]. Doostkam A, et al. Effect of Rutin on Diabetic Auditory Neuropathy in an Experimental Rat Model. Clin Exp Otorhinolaryngol. 2021;14(3):259-267. [Content Brief]

[3]. Habtemariam S. Rutin as a Natural Therapy for Alzheimer's Disease: Insights into its Mechanisms of Action. Curr Med Chem. 2016;23(9):860-873. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5048 mL	7.5238 mL	15.0476 mL	37.6189 mL
	5 mM	0.3010 mL	1.5048 mL	3.0095 mL	7.5238 mL
	10 mM	0.1505 mL	0.7524 mL	1.5048 mL	3.7619 mL
	15 mM	0.1003 mL	0.5016 mL	1.0032 mL	2.5079 mL
	20 mM	0.0752 mL	0.3762 mL	0.7524 mL	1.8809 mL
	25 mM	0.0602 mL	0.3010 mL	0.6019 mL	1.5048 mL
	30 mM	0.0502 mL	0.2508 mL	0.5016 mL	1.2540 mL
	40 mM	0.0376 mL	0.1881 mL	0.3762 mL	0.9405 mL
	50 mM	0.0301 mL	0.1505 mL	0.3010 mL	0.7524 mL
	60 mM	0.0251 mL	0.1254 mL	0.2508 mL	0.6270 mL

Rutin trihydrate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rutin250249-75-3RutosideQuercetin 3-O-rutinosideApoptosisEndogenous MetaboliteAlzheimer'sdiseasebloodbrainbarrierneurodegenerativeInhibitorinhibitorinhibit

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Rutin trihydrate (Synonyms: Rutoside trihydrate; Quercetin 3-O-rutinoside trihydrate)

Customer Review

Other Forms of Rutin trihydrate:

Rutin trihydrate Related Antibodies

30 Publications Citing Use of MCE Rutin trihydrate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Rutin trihydrate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Rutin trihydrate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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