Effect of Rutin on Diabetic Auditory Neuropathy in an Experimental Rat Model

  • Clin Exp Otorhinolaryngol. 2021 Aug;14(3):259-267. doi: 10.21053/ceo.2019.02068.
Aida Doostkam  1 ,  Hossein Mirkhani  2 ,  Kamyar Iravani  3 ,  Saied Karbalay-Doust  4 ,  Kazem Zarei  3
Affiliations
  • 1. Department of Pharmacology, School of Medicine, Shiraz Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • 2. Department of Pharmacology, Medicinal and Natural Products Chemistry Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • 3. Department of Otolaryngology, Otolaryngology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • 4. Department of Anatomy, Histomorphometry and Stereology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
PMID: 32764214 DOI: 10.21053/ceo.2019.02068
Abstract

Objectives: Diabetic auditory neuropathy is a common complication of diabetes mellitus that has a major impact on patients' quality of life. In this study, we assessed the efficacy of rutin in treating diabetic auditory neuropathy in an experimental rat model.

Methods: Forty Sprague-Dawley rats were randomly assigned to the following groups: group 1, control; group 2, diabetic rats; and groups 3-5, rats treated with rutin (at doses of 50, 100, and 150 mg/kg, respectively). We used auditory brain stem response, stereology of the spiral ganglion, and measurements of superoxide dismutase (SOD) and malondialdehyde (MDA) to evaluate the effects of treatment.

Results: Significant improvements in auditory neuropathy were observed in the rutin-treated groups in comparison with the diabetic group (P<0.05). Auditory threshold, wave latency, wave morphology, the volume and number of neurons in the spiral ganglion, and SOD and MDA activity showed improvements following treatment.

Conclusion: Rutin shows promise as a treatment modality for diabetic auditory neuropathy, but more trials are warranted for its clinical application.

Keywords
Animal Model; Auditory Brainstem Response; Diabetic Neuropathy; Histology; Rutin.
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