2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage NF-κB
  3. Endogenous Metabolite Nucleoside Antimetabolite/Analog NF-κB
  4. Uridine

ウリジン  (Synonyms: Uridine; β-Uridine)

製品番号: HY-B1449 純度: 99.97%
COA 取扱説明書 Technical Support

Uridine (β-Uridine) is a nucleoside compound composed of uracil and a ribose ring, which are connected by a β-N1-glycosidic bond.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 58-96-8

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 72 在庫あり
Solution
10 mM * 1 mL in DMSO USD 72 在庫あり
Solid
100 mg $66 在庫あり
500 mg $106 在庫あり
1 g $150 在庫あり
5 g $225 在庫あり
10 g $270 在庫あり
25 g $351 在庫あり
50 g $422 在庫あり
100 g $507 在庫あり
> 100 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 19 publication(s) in Google Scholar

Other Forms of Uridine:

ウリジン 関連抗体

Top Publications Citing Use of Products

    Uridine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 May 10:S2090-1232(25)00309-1.  [Abstract]

    Inhibitory effect of Antimycin A on BHV-1 infection was counteracted by excess uridine (10-100 μM) supplementation: MDBK cells were treated with different concentrations of Antimycin A in the presence of excess uridine at the time of BHV-1 infection (MOI = 0.5). Viral infection was quantified at 24 hpi using a Gaussia Luciferase Flash Assay.

    Uridine purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Nov 25;99(11):e0098525.  [Abstract]

    Exogenous uridine (50 μM) supplementation restores ASFV-P30 protein levels suppressed by brequinar. For all experiments, PAMs were infected with ASFV at MOI = 1, and cell lysates were collected at 24 hpi for Western blotting.

    Uridine purchased from MedChemExpress. Usage Cited in: Cell Investig. 2025 Sep 30.

    Western blot analysis of EMT-related proteins (E-cadherin, N-cadherin, Vimentin, ZEB1, Slug, and Zo-1) in H1299 cells treated with uridine (ERI, 0.25-1 mM) for 48 h.

    Uridine purchased from MedChemExpress. Usage Cited in: Cell Investig. 2025 Sep 30.

    Uridine (0.25-1 mM; 48 h) promotes LUAD cell migration. Wound healing assay was used to detect the migration ability of A549 cells treated with uridine at different concentration for 48 h.Scale bars: 100 μm.

    Uridine purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Mar 7;24(6):5093.

    Uridine obviously weakens the expression of ACSL4, which is markedly raised in LPS group in murine lung tissues.

    Uridine purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Aug:116:108982.  [Abstract]

    The viral VP1 protein and β-actin were detected by western blot. β-actin was used as an internal control. 25, 50, 200, 200 μM uridine (48 h) were added to FMDV infected IBRS-2 cells, when treated with 75 μmol/L brequinar for 48 h.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    NF-κB アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Uridine (β-Uridine) is a nucleoside compound composed of uracil and a ribose ring, which are connected by a β-N1-glycosidic bond[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CCRF-CEM IC50
    >200 μM
    Compound: araU
    Compound was evaluated for its antiproliferative activity on human T-lymphocyte CEM cells
    Compound was evaluated for its antiproliferative activity on human T-lymphocyte CEM cells
    		10.1016/S0960-894X(97)00044-9
    H9 EC50
    10 μg/mL
    Compound: uridine
    Antiviral activity against HIV1 3B infected in human H9 cells after 4 days by p24 antigen ELISA assay
    Antiviral activity against HIV1 3B infected in human H9 cells after 4 days by p24 antigen ELISA assay
    		[PMID: 7775984]
    L1210 IC50
    >200 μM
    Compound: araU
    Tested for its antiproliferative activity on murine leukemia L1210 cells
    Tested for its antiproliferative activity on murine leukemia L1210 cells
    		10.1016/S0960-894X(97)00044-9
    MOLT-4 IC50
    >200 μM
    Compound: araU
    Compound was evaluated for its antiproliferative activity on human T-lymphocyte Molt4/C8 cells
    Compound was evaluated for its antiproliferative activity on human T-lymphocyte Molt4/C8 cells
    		10.1016/S0960-894X(97)00044-9
    RAW264.7 IC50
    >10 μM
    Compound: 21
    Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay
    		[PMID: 28499733]
    体外実験

    Uridine (6-24 mM, 7 days) inhibits the proliferation of leukemia cell HL-60, arrests the cell cycle at G2/M phase, and induces the differentiation of HL-60 into mature cells with characteristics of monocytes and granulocytes[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ウリジン 関連抗体

    Cell Proliferation Assay[1]

    Cell Line: HL-60
    Concentration: 6-24 mM
    Incubation Time: 7 days
    Result: Inhibited the cell proliferation.
    体内実験

    Uridine (10-20 mg/kg, oral, 6 weeks) alleviates CCl4 (HY-Y0298)-induced liver fibrosis, inhibits the expression of pro-inflammatory cytokines and the activation of the NF-κB signaling pathway in mouse liver fibrosis models[2].
    Uridine (30 mg/kg, iv, single dose) exhibits anti-ischemic, antixoxidant and anti-arrhythmic effcts in rats models, exhibits protective efficacy against acute myocardial ischemia and ischemia/reperfusion injury[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mouse liver fibrosis models[2]
    Dosage: 10-20 mg/kg
    Administration: oral, 6 weeks
    Result: Reduced levels of ALP, ALT and AST in serum, decreased the collagen deposition.
    Animal Model: Rats acute myocardial ischemia (AMI) and ischemia/reperfusion (I/R) models[3]
    Dosage: 30 mg/kg
    Administration: iv, single dose
    Result: Inhibited ischemia-induced lipid peroxidation, reduced the ischemic area, duration of ischemia-induced arrhythmias, the occurrence of premature ventricular beats (PVB) and ventricular tachycardia (VT).
    臨床実験
    分子量

    244.20

    分子式

    C9H12N2O6
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@@H](O)[C@H](N2C(NC(C=C2)=O)=O)O[C@@H]1CO
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    Store at room temperature 3 years

    *In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature 3 years)
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 100 mg/mL (409.50 mM)

    DMSO : 50 mg/mL (204.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.0950 mL 20.4750 mL 40.9500 mL
    5 mM 0.8190 mL 4.0950 mL 8.1900 mL
    10 mM 0.4095 mL 2.0475 mL 4.0950 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.24 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (10.24 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 40 mg/mL (163.80 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.97%

    COA (253 KB) RP-HPLC (252 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.0950 mL 20.4750 mL 40.9500 mL 102.3751 mL
    5 mM 0.8190 mL 4.0950 mL 8.1900 mL 20.4750 mL
    10 mM 0.4095 mL 2.0475 mL 4.0950 mL 10.2375 mL
    15 mM 0.2730 mL 1.3650 mL 2.7300 mL 6.8250 mL
    20 mM 0.2048 mL 1.0238 mL 2.0475 mL 5.1188 mL
    25 mM 0.1638 mL 0.8190 mL 1.6380 mL 4.0950 mL
    30 mM 0.1365 mL 0.6825 mL 1.3650 mL 3.4125 mL
    40 mM 0.1024 mL 0.5119 mL 1.0238 mL 2.5594 mL
    50 mM 0.0819 mL 0.4095 mL 0.8190 mL 2.0475 mL
    60 mM 0.0683 mL 0.3413 mL 0.6825 mL 1.7063 mL
    80 mM 0.0512 mL 0.2559 mL 0.5119 mL 1.2797 mL
    100 mM 0.0410 mL 0.2048 mL 0.4095 mL 1.0238 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Uridine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Uridine58-96-8β-UridineEndogenous MetaboliteNucleoside Antimetabolite/AnalogNF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Uridine
    製品番号:
    HY-B1449
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.