N-エチルマレイミド (Synonyms: N-Ethylmaleimide; NEM)

Name: N-Ethylmaleimide
Brand: MedChemExpress
SKU: HY-D0843
Rating: 5.0 (37 reviews)

製品番号: HY-D0843 純度: 99.59%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

N-Ethylmaleimide (NEM) derives from maleic acid, it can alkylates free sulfhydryl. N-Ethylmaleimide is an irreversible cysteine protease inhibitor. N-ethylmaleimide specific inhibits phosphate transport in mitochondria. N-Ethylmaleimide inhibits prolyl endopeptidase with an IC₅₀ value of 6.3 μM. N-Ethylmaleimide can be used to modify cysteine residues in proteins and peptides.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 128-53-0

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 35	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 35	在庫あり	Estimated Time of Arrival: December 31
Solid or liquid
5 mg	$32	在庫あり	Estimated Time of Arrival: December 31
10 mg	$50	在庫あり	Estimated Time of Arrival: December 31
25 mg	$75	在庫あり	Estimated Time of Arrival: December 31
50 mg	$125	在庫あり	Estimated Time of Arrival: December 31
100 mg	$188	在庫あり	Estimated Time of Arrival: December 31
200 mg	$264	在庫あり	Estimated Time of Arrival: December 31
500 mg	$450	在庫あり	Estimated Time of Arrival: December 31
1 g	$630	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 37 publication(s) in Google Scholar

Other Forms of N-Ethylmaleimide:

N-Ethylmaleimide-d₅ 在庫あり
N-Ethylmaleimide (Standard) お問い合わせ

顧客検証

Cell Proliferation/Viability Assay

Flow Cytometry

In Vivo Efficacy Study

IHC

: N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]; MHCC97H SR and Hep3B SR cells were treated with sorafenib for 24 hours, with or without NEM (10 μM) or FSG67 (30 μM), and cell death was assessed by the CCK-8 assay.

: N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]; MHCC97H SR and Hep3B SR cells were treated with sorafenib for 24 hours, with or without NEM (10 μM) or FSG67 (30 μM), and apoptosis was assessed by flow cytometry.

: N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]; The sensitivity of MHCC97H SR cells to sorafenib, NEM (1 mg/kg, every 2 days), and FSG67 was evaluated in vivo. The figure shows a solid view of the tumor.

: N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]; The sensitivity of MHCC97H SR cells to sorafenib, NEM (1 mg/kg, every 2 days), and FSG67 was evaluated in vivo. The figure shows the tumor growth curve.

: N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]; The sensitivity of MHCC97H SR cells to sorafenib, NEM (1 mg/kg, every 2 days), and FSG67 was evaluated in vivo. The figure shows the results of p65 and Oil Red O staining immunohistochemical (IHC) analysis.

Cathepsin アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

Deubiquitinase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15 USP28 USP25

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

IC50: 6.3 μM (prolyl endopeptidase)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	EC₅₀	43.7 3 Compound: NEM	Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins	[PMID: 22061823]
COS-7	EC₅₀	43.7 3 Compound: NEM	Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins	[PMID: 22061823]
KB 3-1	IC₅₀	0.03 2 Compound: N-ethyl maleimide	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
KB 3-1	IC₅₀	0.03 2 Compound: N-ethyl maleimide	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
COS-7	EC₅₀	43.7 3 Compound: NEM	Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins	[PMID: 22061823]
KB-V1	IC₅₀	0.016 2 Compound: N-ethyl maleimide	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]
KB-V1	IC₅₀	0.016 2 Compound: N-ethyl maleimide	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]
MCF7	GI₅₀	9.9 6 Compound: 45	Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
KB 3-1	IC₅₀	0.03 2 Compound: N-ethyl maleimide	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
MCF7	GI₅₀	9.9 6 Compound: 45	Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
NCI-H460	GI₅₀	6.7 6 Compound: 45	Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
NCI-H460	GI₅₀	6.7 6 Compound: 45	Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
KB-V1	IC₅₀	0.016 2 Compound: N-ethyl maleimide	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]
MCF7	GI₅₀	9.9 6 Compound: 45	Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
SF-268	GI₅₀	6.5 6 Compound: 45	Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
SF-268	GI₅₀	6.5 6 Compound: 45	Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
NCI-H460	GI₅₀	6.7 6 Compound: 45	Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
SF-268	GI₅₀	6.5 6 Compound: 45	Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]

体外実験

N-Ethylmaleimide (20 μM;30 min) inhibits Akt Ser-473, Akt Thr-308 , p70S6K, ribosomal protein S6, 4E-BP1, eIF4E, BAD and FKHR-L1 phosphorylation^[2].
N-Ethylmaleimide (20 μM;30 min) affects conversion of pro-caspase-3 in vascular smooth muscle cells^[2].
N-Ethylmaleimide (20 μM;6 h) promotes vascular smooth muscle cells apoptosis^[2].
N-Ethylmaleimide (20 μM;30 min) affects PP2A activity and ROS production in vascular smooth muscle cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	Vascular smooth muscle cells
Concentration:	20 μM
Incubation Time:	2 hours
Result:	Effectively inhibited platelet-derived growth factor-BB (PDGF-BB)-stimulated Akt Ser-473 , Akt Thr-308, p70S6K, ribosomal protein S6, 4E-BP1, BAD and FKHR-L1 phosphorylation with a concentration of 20 μM.

Western Blot Analysis^[2]

Cell Line:	Vascular smooth muscle cells
Concentration:	20 μM
Incubation Time:	2 hours
Result:	Increased of 1.8-fold in the conversion of pro-caspase-3 into active form, and showed better effect with 20 ng/ml PDGF-BB adding.

Apoptosis Analysis^[2]

Cell Line:	Vascular smooth muscle cells
Concentration:	20 μM
Incubation Time:	6 hours
Result:	Induced vascular smooth muscle cells apoptosis by 3-fold, and exhibited 5-fold apoptosis with 20 ng/ml PDGF-BB adding.

Cell Viability Assay^[2]

Cell Line:	Vascular smooth muscle cells
Concentration:	20 μM
Incubation Time:	30 min
Result:	Increased PP2A activity 1.7-flod and increased ROS production 2-fold in vascular smooth muscle cells.

体内実験

N-Ethylmaleimide (10 mg/kg; i.h.) promotes the prevalence situation of mice with acute gastric ulcers^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats with acute gastric ulcers induced by absolute ethanol injection^[3]
Dosage:	10 mg/kg
Administration:	Subcutaneous injection; 10 mg/kg once
Result:	Increased the lesion area of acute gastric ulcers and attenuated the gastroprotective effect of PAG in rats.

臨床実験

分子量

125.13

分子式

C₆H₇NO₂

CAS 番号

128-53-0

Appearance

<43°C Solid,>46°C Liquid

Color

White to light yellow

SMILES

O=C(C=C1)N(CC)C1=O

Initial Source

Microorganisms

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (799.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (399.58 mM; Need ultrasonic)

Ethanol : 12.5 mg/mL (99.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.9917 mL	39.9584 mL	79.9169 mL
5 mM	1.5983 mL	7.9917 mL	15.9834 mL
10 mM	0.7992 mL	3.9958 mL	7.9917 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (16.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (16.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (16.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (799.17 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.89%

Select Batch:

データシート (286 KB) SDS (642 KB)

COA (258 KB) LCMS (94 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Moriyama A, et al. Porcine muscle prolyl endopeptidase and its endogenous substrates. J Biochem. 1988 Jul;104(1):112-7. [Content Brief]

[2]. Yellaturu CR, et al. N-Ethylmaleimide inhibits platelet-derived growth factor BB-stimulated Akt phosphorylation via activation of protein phosphatase 2A. J Biol Chem. 2002 Oct 18;277(42):40148-55. [Content Brief]

[3]. Matsuda H, et al. Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulfhydryls, and prostaglandins in gastroprotection by momordin Ic, an oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesions in rats. Life Sci. 1999;65(2):PL27-32. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / H₂O / DMSO	1 mM	7.9917 mL	39.9584 mL	79.9169 mL	199.7922 mL
	5 mM	1.5983 mL	7.9917 mL	15.9834 mL	39.9584 mL
	10 mM	0.7992 mL	3.9958 mL	7.9917 mL	19.9792 mL
	15 mM	0.5328 mL	2.6639 mL	5.3278 mL	13.3195 mL
	20 mM	0.3996 mL	1.9979 mL	3.9958 mL	9.9896 mL
	25 mM	0.3197 mL	1.5983 mL	3.1967 mL	7.9917 mL
	30 mM	0.2664 mL	1.3319 mL	2.6639 mL	6.6597 mL
	40 mM	0.1998 mL	0.9990 mL	1.9979 mL	4.9948 mL
	50 mM	0.1598 mL	0.7992 mL	1.5983 mL	3.9958 mL
	60 mM	0.1332 mL	0.6660 mL	1.3319 mL	3.3299 mL
	80 mM	0.0999 mL	0.4995 mL	0.9990 mL	2.4974 mL
H₂O / DMSO	100 mM	0.0799 mL	0.3996 mL	0.7992 mL	1.9979 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N-Ethylmaleimide Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Ethylmaleimide128-53-0NEMCathepsinDeubiquitinaseApoptosisDUBsVSMCcysteine residuemodifysulfhydrylphosphate transportInhibitorinhibitorinhibit

日本の代理先をお選びくださいナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
製品名			カスタマ需要量 *
お名前 *			タイトル
メールアドレス *			電話番号 *
デパートメント			組纖名 *
市区町村			都道府県
国或いは地域 *
必ず会社名を記載ください。個人への返信は行いません。
備考

製品名			Lot #
お名前 *			メールアドレス *
電話番号 *			部門 *
組纖名 *			国或いは地域 *
備考

N-エチルマレイミド (Synonyms: N-Ethylmaleimide; NEM)

カスタマーレビュー

Other Forms of N-Ethylmaleimide:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Cathepsin アイソフォーム固有の製品をすべて表示:

Deubiquitinase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

N-Ethylmaleimide Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

N-エチルマレイミド (Synonyms: N-Ethylmaleimide; NEM)

カスタマーレビュー

Other Forms of N-Ethylmaleimide:

N-エチルマレイミド 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Cathepsin アイソフォーム固有の製品をすべて表示:

Deubiquitinase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

N-エチルマレイミド 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

N-Ethylmaleimide Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report

N-エチルマレイミド関連抗体

N-エチルマレイミド関連抗体