Phenoxodiol (Synonyms: Idronoxil; Dehydroequol; Haginin E)

Name: Phenoxodiol
Brand: MedChemExpress
SKU: HY-13721
Rating: 5.0 (3 reviews)

製品番号: HY-13721 純度: 99.89%: Data Sheet SDS COA 取扱説明書
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Technical Support

Phenoxodiol (Idronoxil), a synthetic analog of Genestein, activates the mitochondrial caspase system, inhibits XIAP (an apoptosis inhibitor), and sensitizes the cancer cells to Fas-mediated apoptosis. Phenoxodiol also inhibits DNA topoisomerase II by stabilizing the cleavable complex. Phenoxodiol induces cell cycle arrest in the G1/S phase of the cell cycle and upregulates p21^WAF1 via a p53 independent manner.

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Phenoxodiol 構造式

CAS 番号 : 81267-65-4

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 198	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 198	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$180	在庫あり	Estimated Time of Arrival: December 31
10 mg	$290	在庫あり	Estimated Time of Arrival: December 31
25 mg	$580	在庫あり	Estimated Time of Arrival: December 31
50 mg	$930	在庫あり	Estimated Time of Arrival: December 31
100 mg	$1500	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of Phenoxodiol:

Phenoxodiol (Standard) お問い合わせ

顧客検証

Caspase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Topoisomerase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Topoisomerase Topo I Topo II DNA gyrase

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

Caspase, DNA topoisomerase II^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HMEC-1	GI₅₀	4.1 3 Compound: 1, Isoflavene	Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
HMEC-1	GI₅₀	4.1 3 Compound: 1, Isoflavene	Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
HMEC-1	GI₅₀	4.1 3 Compound: 1, Isoflavene	Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human HMEC1 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MDA-MB-231	GI₅₀	31.3 3 Compound: 1	Growth inhibition of human MDA-MB-231 cells after 72 hrs by Alamar blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MDA-MB-231	GI₅₀	62.9 3 Compound: 1, Isoflavene	Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MDA-MB-231	IC₅₀	33 3 Compound: 1	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by Alamar blue/spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by Alamar blue/spectrophotometric analysis	[PMID: 25189689]
MDA-MB-231	GI₅₀	31.3 3 Compound: 1	Growth inhibition of human MDA-MB-231 cells after 72 hrs by Alamar blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MDA-MB-231	GI₅₀	62.9 3 Compound: 1, Isoflavene	Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MDA-MB-231	IC₅₀	33 3 Compound: 1	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by Alamar blue/spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by Alamar blue/spectrophotometric analysis	[PMID: 25189689]
MRC5	GI₅₀	109 3 Compound: 1, Isoflavene	Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MRC5	IC₅₀	108.5 3 Compound: 1	Cytotoxicity against human MRC5 cells after 72 hrs by Alamar blue assay Cytotoxicity against human MRC5 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MDA-MB-231	GI₅₀	31.3 3 Compound: 1	Growth inhibition of human MDA-MB-231 cells after 72 hrs by Alamar blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MRC5	GI₅₀	109 3 Compound: 1, Isoflavene	Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MRC5	IC₅₀	108.5 3 Compound: 1	Cytotoxicity against human MRC5 cells after 72 hrs by Alamar blue assay Cytotoxicity against human MRC5 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MDA-MB-231	GI₅₀	62.9 3 Compound: 1, Isoflavene	Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
U-87MG ATCC	GI₅₀	>100 3 Compound: 1	Growth inhibition of human U87 cells after 72 hrs by Alamar blue assay Growth inhibition of human U87 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MDA-MB-231	IC₅₀	33 3 Compound: 1	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by Alamar blue/spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by Alamar blue/spectrophotometric analysis	[PMID: 25189689]
U-87MG ATCC	GI₅₀	> 100 3 Compound: 1	Growth inhibition of human U87 cells after 72 hrs by Alamar blue assay Growth inhibition of human U87 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
MRC5	GI₅₀	109 3 Compound: 1, Isoflavene	Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis Antiproliferative activity against human MRC5 cells by measuring metabolic activity of cells after 72 hrs by spectrophotometric analysis	[PMID: 23462711]
MRC5	IC₅₀	108.5 3 Compound: 1	Cytotoxicity against human MRC5 cells after 72 hrs by Alamar blue assay Cytotoxicity against human MRC5 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]
U-87MG ATCC	GI₅₀	> 100 3 Compound: 1	Growth inhibition of human U87 cells after 72 hrs by Alamar blue assay Growth inhibition of human U87 cells after 72 hrs by Alamar blue assay	[PMID: 28408225]

体外実験

Phenoxodiol (Idronoxil) (0-10 μg/mL; 24 h) decreases cell viability of primary ovarian cancer cells^[1].
Phenoxodiol (0-10 μg/mL; 24 h) induces apoptosis and restores sensitivity to Fas-mediated apoptosis in ovarian cancer cells^[1].
Phenoxodiol (0-10 μg/mL; 24 h) induces caspase-8 activation and FLIP downregulation through the Akt-pathway. Phenoxodiol-induced apoptosis involves activation of the mitochondrial pathway and is caspase dependent. Phenoxodiol treatment results in downregulation and cleavage of XIAP^[1].
Phenoxodiol (10 and 30 μM; 24 and 48 h) induces cell cycle arrest in the G1/S phase of the cell cycle in prostate cancer cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	R182s, R127, Hey, CP70, A2780, R187, R188, R207 and OSE cells
Concentration:	0, 0.01, 0.1, 1 and 10 μg/mL
Incubation Time:	24 h
Result:	A significant decrease in cell viabilityin all the ovarian cancer cell cultures was observed at a concentration of 10 μg/mL (41.6 μM) and did not affect ovarian surface epithelial (OSE) cells’ viability. In CP70 cells, the IC₅₀ was 1.35 μM.

Apoptosis Analysis^[1]

Cell Line:	CP70 and OSE cells
Concentration:	10 μg/mL
Incubation Time:	24 h
Result:	Induced apoptosis and resulted in a twofold increase in caspase-3 activity. No change in caspase-3 activity was found in normal OSE cells.

Western Blot Analysis^[1]

Cell Line:	Ovarian cancer cells
Concentration:	10 μg/mL
Incubation Time:	24 h
Result:	Induced caspase-8 activation, characterized bycleavage of procaspase-8 into its p43/41 and p28 forms and in downregulation of the p43 form of FLIPC in all the primarycultures as well as in the CP70 and Hey cell lines. Decreased the levels of Akt expression. Resulted in XIAP downregulation and cleavage to its 30 kDa inactive form.

Cell Cycle Analysis^[2]

Cell Line:	LNCaP, DU145 and PC3 cells
Concentration:	10 and 30 μM
Incubation Time:	24 and 48 h
Result:	Induced significantly decreased G2 phase cell populations versus DMSO vehicle control, over 24 hours for both 10 μM and 30 μM treatments. The S phase cell population was found to increase versus DMSO vehicle control.

RT-PCR^[2]

Cell Line:	LNCaP, DU145 and PC3 cells
Concentration:	10 and 30 μM
Incubation Time:	24 and 48 h
Result:	PC3 cells were found to significantly increase the expression of c-Myc at 30 μM after 48 h. Decreased the expression of Cyclin-D1 after 24 hours of treatment with 30 μM in DU145 and PC3 cells. Decreased the expression of Ki-67 after 24 hours of treatment with 10 and 30 μM in LNCaP and PC3 cells. Increased the expression of p21 in LNCaP and PC3 cells.

臨床実験

分子量

240.25

分子式

C₁₅H₁₂O₃

CAS 番号

81267-65-4

Appearance

Solid

Color

Off-white to brown

SMILES

OC1=CC=C2C=C(C3=CC=C(O)C=C3)COC2=C1

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : ≥ 100 mg/mL (416.23 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1623 mL	20.8117 mL	41.6233 mL
5 mM	0.8325 mL	4.1623 mL	8.3247 mL
10 mM	0.4162 mL	2.0812 mL	4.1623 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.89%

Select Batch:

データシート (277 KB) SDS (396 KB)

COA (246 KB) HNMR (231 KB) LCMS (91 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Kamsteeg M, et al. Phenoxodiol--an isoflavone analog--induces apoptosis in chemoresistant ovarian cancer cells. Oncogene. 2003 May 1;22(17):2611-20. [Content Brief]

[2]. Mahoney S, et al. The effects of phenoxodiol on the cell cycle of prostate cancer cell lines. Cancer Cell Int. 2014 Nov 8;14(1):110. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1623 mL	20.8117 mL	41.6233 mL	104.0583 mL
	5 mM	0.8325 mL	4.1623 mL	8.3247 mL	20.8117 mL
	10 mM	0.4162 mL	2.0812 mL	4.1623 mL	10.4058 mL
	15 mM	0.2775 mL	1.3874 mL	2.7749 mL	6.9372 mL
	20 mM	0.2081 mL	1.0406 mL	2.0812 mL	5.2029 mL
	25 mM	0.1665 mL	0.8325 mL	1.6649 mL	4.1623 mL
	30 mM	0.1387 mL	0.6937 mL	1.3874 mL	3.4686 mL
	40 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6015 mL
	50 mM	0.0832 mL	0.4162 mL	0.8325 mL	2.0812 mL
	60 mM	0.0694 mL	0.3469 mL	0.6937 mL	1.7343 mL
	80 mM	0.0520 mL	0.2601 mL	0.5203 mL	1.3007 mL
	100 mM	0.0416 mL	0.2081 mL	0.4162 mL	1.0406 mL

Phenoxodiol Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenoxodiol81267-65-4Idronoxil Dehydroequol Haginin ECaspaseApoptosisTopoisomeraseovarian cancerCP70prostate cancerXIAPInhibitorinhibitorinhibit

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備考

Phenoxodiol (Synonyms: Idronoxil; Dehydroequol; Haginin E)

カスタマーレビュー

Other Forms of Phenoxodiol:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Caspase アイソフォーム固有の製品をすべて表示:

Topoisomerase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Phenoxodiol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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Phenoxodiol (Synonyms: Idronoxil; Dehydroequol; Haginin E)

カスタマーレビュー

Other Forms of Phenoxodiol:

Phenoxodiol 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Caspase アイソフォーム固有の製品をすべて表示:

Topoisomerase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Phenoxodiol 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Phenoxodiol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report