Capivasertib (Synonyms: AZD5363)

Name: Capivasertib
Brand: MedChemExpress
SKU: HY-15431
Rating: 5.0 (81 reviews)

製品番号: HY-15431 純度: 99.96%: Data Sheet SDS COA 取扱説明書
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Capivasertib (AZD5363) is an orally active and potent pan-AKT kinase inhibitor with IC₅₀ of 3, 7 and 7 nM for Akt1,Akt2 and Akt3, respectively.

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CAS 番号 : 1143532-39-1

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 105	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 105	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$96	在庫あり	Estimated Time of Arrival: December 31
10 mg	$144	在庫あり	Estimated Time of Arrival: December 31
25 mg	$260	在庫あり	Estimated Time of Arrival: December 31
50 mg	$360	在庫あり	Estimated Time of Arrival: December 31
100 mg	$480	在庫あり	Estimated Time of Arrival: December 31
200 mg	$725	在庫あり	Estimated Time of Arrival: December 31
500 mg		お問い合わせ
1 g		お問い合わせ

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カスタマーレビュー

Based on 81 publication(s) in Google Scholar

Other Forms of Capivasertib:

(R)-Capivasertib 在庫あり
Capivasertib-d₄ お問い合わせ
Capivasertib (Standard) お問い合わせ

顧客検証

Histological Imaging/Staining

IHC

Cell Proliferation/Viability Assay

: Capivasertib purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 3:e2411719. [Abstract]; Mice were treated with AZD5363 (100 mg/kg, once daily) by oral gavage. Representative images of pAKT1 (Ser473) (upper panel) and Ki67 (lower panel) staining of subcutaneous xenograft tumors were shown.

: Capivasertib purchased from MedChemExpress. Usage Cited in: Neuro Oncol. 2024 Jun 3;26(6):1052-1066. [Abstract]; Immunoblot of PC9 cell signaling response to 24 h treatment with indicated concentrations of Capivasertib (0, 0.1, 0.5, 1, 5, 10, 25, 50, 100 μM) showed a dose-dependent decrease in phosphorylated AKT levels.

: Capivasertib purchased from MedChemExpress. Usage Cited in: J Exp Med. 2024 Mar 4;221(3):e20232028. [Abstract]; Western blot analyses of Club cell-derived EML4-ALK L1196M tumoroids were treated with indicated doses of capivasertib (Cap) (0, 1, 10, 100, 1000, 10000 nM) for 12 h and then subjected to western blot analyses.

: Capivasertib purchased from MedChemExpress. Usage Cited in: Engineering. 28 October 2022.; Lipid accumulation in adipocytes after the inhibition of Akt activity with capivasertib (Capi) (5 μM, six days) was measured with oil red O staining.

: Capivasertib purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Aug 25;12(1):5112. [Abstract]; The effect of AKT inhibitor (AKTi, capivasertib, 500 nM in the MCF-7 cell model) and CellTiter-Blue viabilityassay performed over 6 days.

Akt アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Akt Akt1 Akt2 Akt3

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Capivasertib (AZD5363) is an orally active and potent pan-AKT kinase inhibitor with IC₅₀ of 3, 7 and 7 nM for Akt1,Akt2 and Akt3, respectively.

IC₅₀ & Target^[1]

Akt1

3 nM (IC₅₀)

Akt2

7 nM (IC₅₀)

Akt3

7 nM (IC₅₀)

P70S6K

6 nM (IC₅₀)

PKA

7 nM (IC₅₀)

ROCK2

60 nM (IC₅₀)

ROCK1

470 nM (IC₅₀)

Autophagy

Cellular Effect

Cell Line	Type	Value	Description	References
769-P	IC₅₀	10.75 3 Compound: AZD5363	Antiproliferative activity against human 769-P cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human 769-P cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
769-P	IC₅₀	10.75 3 Compound: AZD5363	Antiproliferative activity against human 769-P cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human 769-P cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
769-P	IC₅₀	10.75 3 Compound: AZD5363	Antiproliferative activity against human 769-P cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human 769-P cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
786-0	IC₅₀	23.28 3 Compound: AZD5363	Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
786-0	IC₅₀	23.28 3 Compound: AZD5363	Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
786-0	IC₅₀	23.28 3 Compound: AZD5363	Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
A549	IC₅₀	23.67 3 Compound: AZD5363	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
A549	IC₅₀	23.67 3 Compound: AZD5363	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
AGS	IC₅₀	9.6 3 Compound: AZD5363	Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
AGS	IC₅₀	9.6 3 Compound: AZD5363	Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
A549	IC₅₀	23.67 3 Compound: AZD5363	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
Bel-7402	IC₅₀	49.6 3 Compound: AZD5363	Antiproliferative activity against human Bel-7402 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human Bel-7402 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
Bel-7402	IC₅₀	49.6 3 Compound: AZD5363	Antiproliferative activity against human Bel-7402 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human Bel-7402 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
AGS	IC₅₀	9.6 3 Compound: AZD5363	Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
CHO	IC₅₀	>100 3 Compound: 64, AZD5363	Inhibition of human ERG expressed in CHO cells by ionworks assay Inhibition of human ERG expressed in CHO cells by ionworks assay	[PMID: 23394218]
CHO	IC₅₀	> 100000 1 Compound: 64, AZD5363	Inhibition of human ERG expressed in CHO cells by ionworks assay Inhibition of human ERG expressed in CHO cells by ionworks assay	[PMID: 23394218]
AN3-CA	EC₅₀	869 1 Compound: Capivasertib	Antiproliferative activity against human AN3-CA cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human AN3-CA cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
HaCaT	IC₅₀	4.2 3 Compound: AZD5363	Antiproliferative activity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay Antiproliferative activity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay	[PMID: 34375113]
HaCaT	IC₅₀	4.2 3 Compound: AZD5363	Antiproliferative activity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay Antiproliferative activity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay	[PMID: 34375113]
AN3-CA	EC₅₀	869 1 Compound: Capivasertib	Antiproliferative activity against human AN3-CA cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay Antiproliferative activity against human AN3-CA cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 31584233]
HCT-116	IC₅₀	5.2 3 Compound: AZD5363	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 27089211]
HCT-116	IC₅₀	5.2 3 Compound: AZD5363	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 31298542]
BT-474	EC₅₀	1605 1 Compound: Capivasertib	Antiproliferative activity against human BT-474 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human BT-474 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
HCT-116	IC₅₀	5.2 3 Compound: AZD5363	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 27089211]
Bel-7402	IC₅₀	49.6 3 Compound: AZD5363	Antiproliferative activity against human Bel-7402 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human Bel-7402 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
HCT-116	IC₅₀	5.2 3 Compound: AZD5363	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 31298542]
HepG2	IC₅₀	6.7 3 Compound: AZD5363	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
HepG2	IC₅₀	6.7 3 Compound: AZD5363	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
CHO	IC₅₀	> 100000 1 Compound: 64, AZD5363	Inhibition of human ERG expressed in CHO cells by ionworks assay Inhibition of human ERG expressed in CHO cells by ionworks assay	[PMID: 23394218]
HT-29	IC₅₀	36.92 3 Compound: AZD5363	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
HT-29	IC₅₀	36.92 3 Compound: AZD5363	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
KHOS/NP	IC₅₀	40.42 3 Compound: AZD5363	Antiproliferative activity against human KHOS cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human KHOS cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
KHOS/NP	IC₅₀	40.42 3 Compound: AZD5363	Antiproliferative activity against human KHOS cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human KHOS cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
HCT-116	IC₅₀	5.2 3 Compound: AZD5363	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 27089211]
LNCaP	IC₅₀	0.178 3 Compound: AZD5363	Antiproliferative activity against human LNCAP cells Antiproliferative activity against human LNCAP cells	[PMID: 31298542]
LNCaP	IC₅₀	0.46 3 Compound: AZD5363	Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
HCT-116	IC₅₀	5.2 3 Compound: AZD5363	Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT116 cells after 72 hrs by SRB assay	[PMID: 31298542]
LNCaP	IC₅₀	336.8 1 Compound: AZD5363	Inhibition of Akt in human PTEN-null LNCAP cells assessed as suppression in PRAS40 phosphorylation after 1 hr by ELISA analysis Inhibition of Akt in human PTEN-null LNCAP cells assessed as suppression in PRAS40 phosphorylation after 1 hr by ELISA analysis	[PMID: 27089211]
LNCaP	IC₅₀	0.46 3 Compound: AZD5363	Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
MCF7	IC₅₀	4.2 3 Compound: AZD5363	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
LNCaP	IC₅₀	0.06 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of GSK3beta Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of GSK3beta	[PMID: 23394218]
HT-29	IC₅₀	36.92 3 Compound: AZD5363	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
LNCaP	IC₅₀	0.22 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of PRAS40 Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of PRAS40	[PMID: 23394218]
MDA-MB-231	IC₅₀	60.52 3 Compound: AZD5363	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
MCF7	IC₅₀	4.2 3 Compound: AZD5363	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
MDA-MB-468	GI₅₀	3.8 3 Compound: AZD5363	Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth by measured after 5 days by IncuCyte zoom live cell analysis Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth by measured after 5 days by IncuCyte zoom live cell analysis	[PMID: 35119851]
MDA-MB-231	IC₅₀	60.52 3 Compound: AZD5363	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
MDA-MB-468	IC₅₀	89 1 Compound: 64, AZD5363	Inhibition of Akt in human MDA-MB-468 cells assessed as inhibition of GSK3beta phosphorylation after 2 hrs by laser scanning cytometry Inhibition of Akt in human MDA-MB-468 cells assessed as inhibition of GSK3beta phosphorylation after 2 hrs by laser scanning cytometry	[PMID: 23394218]
MDA-MB-468	GI₅₀	3.8 3 Compound: AZD5363	Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth by measured after 5 days by IncuCyte zoom live cell analysis Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth by measured after 5 days by IncuCyte zoom live cell analysis	[PMID: 35119851]
NCI-H1975	IC₅₀	2.7 3 Compound: AZD5363	Antiproliferative activity against human NCI-H1975 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H1975 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
NCI-H1975	IC₅₀	8.23 3 Compound: AZD5363	Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
MDA-MB-468	IC₅₀	0.38 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of GSK3beta Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of GSK3beta	[PMID: 23394218]
MDA-MB-468	IC₅₀	0.39 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of PRAS40 Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of PRAS40	[PMID: 23394218]
OS-RC-2	IC₅₀	53.43 3 Compound: AZD5363	Antiproliferative activity against human OS-RC-2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human OS-RC-2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
MDA-MB-468	IC₅₀	89 1 Compound: 64, AZD5363	Inhibition of Akt in human MDA-MB-468 cells assessed as inhibition of GSK3beta phosphorylation after 2 hrs by laser scanning cytometry Inhibition of Akt in human MDA-MB-468 cells assessed as inhibition of GSK3beta phosphorylation after 2 hrs by laser scanning cytometry	[PMID: 23394218]
OVCAR-8	IC₅₀	4.48 3 Compound: AZD5363	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
NCI-H1975	IC₅₀	8.23 3 Compound: AZD5363	Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OVCAR-8	IC₅₀	7.27 3 Compound: AZD5363	Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay	[PMID: 27089211]
PC-3	GI₅₀	6.5 3 Compound: AZD5363	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 5 days by IncuCyte zoom live cell analysis Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 5 days by IncuCyte zoom live cell analysis	[PMID: 35119851]
NCI-H1975	IC₅₀	2.7 3 Compound: AZD5363	Antiproliferative activity against human NCI-H1975 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H1975 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
LNCaP	IC₅₀	336.8 1 Compound: AZD5363	Inhibition of Akt in human PTEN-null LNCAP cells assessed as suppression in PRAS40 phosphorylation after 1 hr by ELISA analysis Inhibition of Akt in human PTEN-null LNCAP cells assessed as suppression in PRAS40 phosphorylation after 1 hr by ELISA analysis	[PMID: 27089211]
PC-3	IC₅₀	82.57 3 Compound: AZD5363	Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OS-RC-2	IC₅₀	53.43 3 Compound: AZD5363	Antiproliferative activity against human OS-RC-2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human OS-RC-2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
SK-OV-3	IC₅₀	6.32 3 Compound: AZD5363	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
SMMC-7721	IC₅₀	84.12 3 Compound: AZD5363	Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OVCAR-8	IC₅₀	7.27 3 Compound: AZD5363	Antiproliferative activity against human OVCAR8 cells Antiproliferative activity against human OVCAR8 cells	[PMID: 31298542]
OVCAR-8	IC₅₀	7.27 3 Compound: AZD5363	Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay	[PMID: 27089211]
U-251	IC₅₀	14.22 3 Compound: AZD5363	Antiproliferative activity against human U251 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human U251 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OVCAR-8	IC₅₀	4.48 3 Compound: AZD5363	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
U-87MG ATCC	IC₅₀	50.6 3 Compound: AZD5363	Antiproliferative activity against human U-87MG cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human U-87MG cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
PC-3	GI₅₀	6.5 3 Compound: AZD5363	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 5 days by IncuCyte zoom live cell analysis Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 5 days by IncuCyte zoom live cell analysis	[PMID: 35119851]
PC-3	IC₅₀	82.57 3 Compound: AZD5363	Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
RT-4	IC₅₀	5 3 Compound: 64, AZD5363	Inhibition of P70S6K in TSC1 deficient human RT4 cells assessed as S6 phosphorylation Inhibition of P70S6K in TSC1 deficient human RT4 cells assessed as S6 phosphorylation	[PMID: 23394218]
HaCaT	IC₅₀	4.2 3 Compound: AZD5363	Antiproliferative activity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay Antiproliferative activity against human HaCaT cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay	[PMID: 34375113]
RT-4	IC₅₀	1 3 Compound: 64, AZD5363	Inhibition of PKA in TSC1 deficient human RT4 cells assessed as S6 phosphorylation Inhibition of PKA in TSC1 deficient human RT4 cells assessed as S6 phosphorylation	[PMID: 23394218]
HepG2	IC₅₀	6.7 3 Compound: AZD5363	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
SK-OV-3	IC₅₀	6.32 3 Compound: AZD5363	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
SMMC-7721	IC₅₀	84.12 3 Compound: AZD5363	Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
U-251	IC₅₀	14.22 3 Compound: AZD5363	Antiproliferative activity against human U251 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human U251 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
U-87MG ATCC	IC₅₀	50.6 3 Compound: AZD5363	Antiproliferative activity against human U-87MG cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human U-87MG cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
Vero	CC₅₀	82 3 Compound: Capivasertib	Cytotoxicity in African green monkey Vero cells by MTT assay Cytotoxicity in African green monkey Vero cells by MTT assay	[PMID: 36857133]
KHOS/NP	IC₅₀	40.42 3 Compound: AZD5363	Antiproliferative activity against human KHOS cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human KHOS cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
KU-19-19	EC₅₀	20866 1 Compound: Capivasertib	Antiproliferative activity against human KU-19-19 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human KU-19-19 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
LNCaP	IC₅₀	0.06 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of GSK3beta Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of GSK3beta	[PMID: 23394218]
LNCaP	IC₅₀	0.178 3 Compound: AZD5363	Antiproliferative activity against human LNCAP cells Antiproliferative activity against human LNCAP cells	[PMID: 31298542]
LNCaP	IC₅₀	0.22 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of PRAS40 Inhibition of Akt in PTEN-deficient human LNCaP cells assessed as phosphorylation of PRAS40	[PMID: 23394218]
LNCaP	IC₅₀	0.46 3 Compound: AZD5363	Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
LNCaP	IC₅₀	336.8 1 Compound: AZD5363	Inhibition of Akt in human PTEN-null LNCAP cells assessed as suppression in PRAS40 phosphorylation after 1 hr by ELISA analysis Inhibition of Akt in human PTEN-null LNCAP cells assessed as suppression in PRAS40 phosphorylation after 1 hr by ELISA analysis	[PMID: 27089211]
MCF7	EC₅₀	555 1 Compound: Capivasertib	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
MCF7	IC₅₀	4.2 3 Compound: AZD5363	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
MDA-MB-231	IC₅₀	3 1 Compound: AZD5363	Inhibition of AKT phosphorylation in human MDA-MB-231 incubated for 18 hrs by Western blot analysis Inhibition of AKT phosphorylation in human MDA-MB-231 incubated for 18 hrs by Western blot analysis	[PMID: 38592948]
MDA-MB-231	IC₅₀	60.52 3 Compound: AZD5363	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
MDA-MB-468	GI₅₀	3.8 3 Compound: AZD5363	Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth by measured after 5 days by IncuCyte zoom live cell analysis Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth by measured after 5 days by IncuCyte zoom live cell analysis	[PMID: 35119851]
MDA-MB-468	IC₅₀	0.38 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of GSK3beta Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of GSK3beta	[PMID: 23394218]
MDA-MB-468	IC₅₀	0.39 3 Compound: 64, AZD5363	Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of PRAS40 Inhibition of Akt in PTEN-deficient human MDA-MB-468 cells assessed as phosphorylation of PRAS40	[PMID: 23394218]
MDA-MB-468	IC₅₀	89 1 Compound: 64, AZD5363	Inhibition of Akt in human MDA-MB-468 cells assessed as inhibition of GSK3beta phosphorylation after 2 hrs by laser scanning cytometry Inhibition of Akt in human MDA-MB-468 cells assessed as inhibition of GSK3beta phosphorylation after 2 hrs by laser scanning cytometry	[PMID: 23394218]
NCI-H1975	IC₅₀	2.7 3 Compound: AZD5363	Antiproliferative activity against human NCI-H1975 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H1975 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34917257]
NCI-H1975	IC₅₀	8.23 3 Compound: AZD5363	Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OS-RC-2	IC₅₀	53.43 3 Compound: AZD5363	Antiproliferative activity against human OS-RC-2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human OS-RC-2 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OVCAR-8	IC₅₀	4.48 3 Compound: AZD5363	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
OVCAR-8	IC₅₀	7.27 3 Compound: AZD5363	Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay	[PMID: 27089211]
OVCAR-8	IC₅₀	7.27 3 Compound: AZD5363	Antiproliferative activity against human OVCAR8 cells Antiproliferative activity against human OVCAR8 cells	[PMID: 31298542]
PC-3	GI₅₀	6.5 3 Compound: AZD5363	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 5 days by IncuCyte zoom live cell analysis Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 5 days by IncuCyte zoom live cell analysis	[PMID: 35119851]
PC-3	IC₅₀	82.57 3 Compound: AZD5363	Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
RT-4	IC₅₀	1 3 Compound: 64, AZD5363	Inhibition of PKA in TSC1 deficient human RT4 cells assessed as S6 phosphorylation Inhibition of PKA in TSC1 deficient human RT4 cells assessed as S6 phosphorylation	[PMID: 23394218]
RT-4	IC₅₀	5 3 Compound: 64, AZD5363	Inhibition of P70S6K in TSC1 deficient human RT4 cells assessed as S6 phosphorylation Inhibition of P70S6K in TSC1 deficient human RT4 cells assessed as S6 phosphorylation	[PMID: 23394218]
SK-OV-3	IC₅₀	6.32 3 Compound: AZD5363	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
SMMC-7721	IC₅₀	84.12 3 Compound: AZD5363	Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
T47D	EC₅₀	475 1 Compound: Capivasertib	Antiproliferative activity against human T47D cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human T47D cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
T47D	EC₅₀	475 1 Compound: Capivasertib	Antiproliferative activity against human T47D cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay Antiproliferative activity against human T47D cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 31584233]
U-251	IC₅₀	14.22 3 Compound: AZD5363	Antiproliferative activity against human U251 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human U251 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
U-87MG ATCC	IC₅₀	50.6 3 Compound: AZD5363	Antiproliferative activity against human U-87MG cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method Antiproliferative activity against human U-87MG cells assessed as inhibition of cell growth incubated for 72 hrs by SRB method	[PMID: 34375113]
Vero	CC₅₀	82 3 Compound: Capivasertib	Cytotoxicity in African green monkey Vero cells by MTT assay Cytotoxicity in African green monkey Vero cells by MTT assay	[PMID: 36857133]
ZR-75-1	EC₅₀	191 1 Compound: Capivasertib	Antiproliferative activity against human ZR-75-1 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human ZR-75-1 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
ZR-75-1	EC₅₀	191 1 Compound: Capivasertib	Antiproliferative activity against human ZR-75-1 cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay Antiproliferative activity against human ZR-75-1 cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 31584233]

体外実験

Capivasertib, a novel pyrrolopyrimidine-derived compound, inhibits all AKT isoforms with a potency of 10 nM or less. Capivasertib inhibits phosphorylation of these substrates with an IC₅₀ value of 0.06 to 0.76 μM in the 3 cell lines. Capivasertib effectively inhibits phosphorylation of S6 and 4E-BP1 in these cell lines, whereas it increases phosphorylation of AKT at both ser⁴⁷³ and thr³⁰⁸. In BT474c cells, Capivasertib induces FOXO3a nuclear translocation with EC₅₀ value of 0.69 μM; a concentration of 3 μM is sufficient to almost completely localize FOXO3a to the nucleus. AZD5363Capivasertibhibitor MK-2206 is much less active (IC₅₀>30 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Oral dosing of Capivasertib (AZD5363) to nude mice causes dose- and time-dependent reduction of PRAS40, GSK3β, and S6 phosphorylation in BT474c xenografts (PRAS40 phosphorylation EC₅₀ ~0.1 μM total plasma exposure), reversible increases in blood glucose concentrations, and dose-dependent decreases in 2[¹⁸F]fluoro-2-deoxy-D-glucose (¹⁸F-FDG) uptake in U87-MG xenografts. Chronic oral dosing of Capivasertib caused dose-dependent growth inhibition of xenografts derived from various tumor types, including HER2⁺ breast cancer models. Capivasertib also significantly enhances the antitumor activity of RP-56976 and GW572016 in breast cancer xenografts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

428.92

分子式

C₂₁H₂₅ClN₆O₂

CAS 番号

1143532-39-1

Appearance

Solid

Color

Off-white to gray

SMILES

O=C(C1(N)CCN(C2=C3C(NC=C3)=NC=N2)CC1)N[C@H](C4=CC=C(Cl)C=C4)CCO

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 125 mg/mL (291.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3314 mL	11.6572 mL	23.3144 mL
5 mM	0.4663 mL	2.3314 mL	4.6629 mL
10 mM	0.2331 mL	1.1657 mL	2.3314 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (23.31 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.96%

Select Batch:

データシート (284 KB) SDS (396 KB)

COA (255 KB) HNMR (127 KB) RP-HPLC (245 KB) MS (199 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Davies BR, et al. Preclinical pharmacology of AZD5363, an inhibitor of AKT: pharmacodynamics, antitumor activity, and correlation of monotherapy activity with genetic background. Mol Cancer Ther. 2012 Apr;11(4):873-87. [Content Brief]

細胞実験 ^[1]	Cell proliferation assay is determined by 2 methods, MTS and Sytox Green. Briefly, cells are seeded in 96-well plates (at a density to allow for logarithmic growth during the 72-hour assay) and incubated overnight at 37°C, 5% CO₂. Cells are then exposed to concentrations of Capivasertib ranging from 30 to 0.003 μM for 72 hours. For the MTS endpoint, cell proliferation is measured by the CellTiter AQueous Non-Radioactive Cell Proliferation Assay reagent. Absorbance is measured with a Tecan Ultra instrument. For the Sytox Green endpoint, Sytox Green nucleic acid dye diluted in TBS-EDTA buffer is added to cells (final concentration of 0.13 μM) and the number of dead cells detected using an Acumen Explorer. Cells are then permeabilized by the addition of saponin (0.03% final concentration, diluted in TBS-EDTA buffer), incubated overnight and a total cell count measured. Predose measurements are made for both MTS and Sytox Green endpoints, and concentration needed to reduce the growth of treated cells to half that of untreated cells (GI₅₀) values are determined using absorbance readings (MTS) or live cell counts^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[1]	Mice^[1] Specific, pathogen-free, female nude mice (nu/nu: Alpk) and male SCID mice (SCID/CB17; 786-0 xenograft studies) are used. When mean tumor sizes reach approximately 0.2 cm3, the mice are randomized into control and treatment groups. The treatment groups received varying dose schedules of Capivasertib (AZD5363) solubilized in a 10% DMSO 25% w/v Kleptose HPB (Roquette) buffer by oral gavage, RP-56976 solubilized in 2.6% ethanol in injectable water by intravenous injection once on day 1 at 15 or 5 mg/kg once weekly. When administered in combination, RP-56976 is administered 1 hour before the oral dose of Capivasertib (AZD5363). The control group received the DMSO/Kleptose buffer alone, twice daily by oral gavage. Tumor volumes (measured by caliper), animal body weight, and tumor condition are recorded twice weekly for the duration of the study. Mice are sacrificed by CO2 euthanasia. The tumor volume is calculated (taking length to be the longest diameter across the tumor and width to be the corresponding perpendicular diameter) using the formula: (length×width)×√(length×width)×(π/6). Growth inhibition from the start of treatment is assessed by comparison of the differences in tumor volume between control and treated groups. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Davies BR, et al. Preclinical pharmacology of AZD5363, an inhibitor of AKT: pharmacodynamics, antitumor activity, and correlation of monotherapy activity with genetic background. Mol Cancer Ther. 2012 Apr;11(4):873-87. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3314 mL	11.6572 mL	23.3144 mL	58.2859 mL
	5 mM	0.4663 mL	2.3314 mL	4.6629 mL	11.6572 mL
	10 mM	0.2331 mL	1.1657 mL	2.3314 mL	5.8286 mL
	15 mM	0.1554 mL	0.7771 mL	1.5543 mL	3.8857 mL
	20 mM	0.1166 mL	0.5829 mL	1.1657 mL	2.9143 mL
	25 mM	0.0933 mL	0.4663 mL	0.9326 mL	2.3314 mL
	30 mM	0.0777 mL	0.3886 mL	0.7771 mL	1.9429 mL
	40 mM	0.0583 mL	0.2914 mL	0.5829 mL	1.4571 mL
	50 mM	0.0466 mL	0.2331 mL	0.4663 mL	1.1657 mL
	60 mM	0.0389 mL	0.1943 mL	0.3886 mL	0.9714 mL
	80 mM	0.0291 mL	0.1457 mL	0.2914 mL	0.7286 mL
	100 mM	0.0233 mL	0.1166 mL	0.2331 mL	0.5829 mL

Capivasertib Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Capivasertib1143532-39-1AZD5363AZD 5363AZD-5363AktAutophagyPKBProtein kinase BInhibitorinhibitorinhibit

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Capivasertib (Synonyms: AZD5363)

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Capivasertib (Synonyms: AZD5363)

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Other Forms of Capivasertib:

Capivasertib 関連抗体

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おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

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生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Capivasertib 関連抗体

Molarity Calculator

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Complete Stock Solution Preparation Table

Capivasertib Related Classifications

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