Hesperetin

Name: Hesperetin
Brand: MedChemExpress
SKU: HY-N0168
Rating: 5.0 (25 reviews)

製品番号: HY-N0168 純度: 99.12%: Data Sheet SDS COA 取扱説明書
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Hesperetin is a natural flavanone that can be found in citrus, and acts as a potent and orally active broad-spectrum inhibitor against human UGT activity. Hesperetin induces apoptosis via p38 MAPK activation. Hesperetin displays a range of bioactivities including antioxidant, anti-inflammatory, and anti-cancer. Hesperetin is found to induce cell-cycle arrest at G2/M phase. Hesperetin can reduce Bcl-2 and enhance BaxM. Hesperetin induces apoptosis through inhibiting NF-κB receptor.

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CAS 番号 : 520-33-2

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 72	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 72	在庫あり	Estimated Time of Arrival: December 31
Solid
50 mg	$66	在庫あり	Estimated Time of Arrival: December 31
100 mg	$80	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 25 publication(s) in Google Scholar

Other Forms of Hesperetin:

(Rac)-Hesperetin 在庫あり
(Rac)-Hesperetin-d₃ お問い合わせ
Hesperetin-d₃ お問い合わせ

顧客検証

: Hesperetin purchased from MedChemExpress. Usage Cited in: Nutr Cancer. 2020;72(3):538-545. [Abstract]; U-251 cells are cultured with indicated concentration of Hesperetin for 24 h. Then the cells are stained with DAPI and observed under a fluorescence microscope.

: Hesperetin purchased from MedChemExpress. Usage Cited in: Nutr Cancer. 2020;72(3):538-545. [Abstract]; The expression of Bax and Bcl-2 was examined by western blotting. GAPDH was served as a loading control.

: Hesperetin purchased from MedChemExpress. Usage Cited in: Nutr Cancer. 2020;72(3):538-545. [Abstract]; Hesperetin induced G2/M arrest in GBM cell. Cells are treated with vehicle or various doses of hesperetin for 24 h. Whole cell lysates are immunoblotted with indicated antibodies. The protein levels of p21, CDK1, and cyclin B1 are measured by western blotting.

: Hesperetin purchased from MedChemExpress. Usage Cited in: Nutr Cancer. 2020;72(3):538-545. [Abstract]; p38 MAPK activation is involved in Hesperetin-induced apoptosis. The phosphorylation of p38 MAPK, JNK, and ERK in U-21 cells treated with hesperetin is analyzed by western blotting.

p38 MAPK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

NF-κB アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

生物活性
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参考文献
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製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
CCRF-CEM	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
Caco-2	IC₅₀	66.67 μM Compound: 28	Cytotoxicity in human Caco2 cells after 72 hrs by MTT assay Cytotoxicity in human Caco2 cells after 72 hrs by MTT assay	[PMID: 28793973]
HCT-116	GI₅₀	36.1 μM Compound: Hesperetin	Anticlonogenic activity in human HCT116 cells after 6 days by crystal violet staining based assay Anticlonogenic activity in human HCT116 cells after 6 days by crystal violet staining based assay	[PMID: 27840137]
HeLa	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
MCF7	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
Monocyte	IC₅₀	2.1 x 10^-6 M Compound: hesperetin	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
PC-3	IC₅₀	40 μM Compound: Hesperetin	Antiproliferative activity against human PC3 cells after 48 hrs by MTT assay Antiproliferative activity against human PC3 cells after 48 hrs by MTT assay	[PMID: 31398616]
PC-3	IC₅₀	90 μM Compound: Hesperetin	Antiproliferative activity against human PC3 cells after 24 hrs by MTT assay Antiproliferative activity against human PC3 cells after 24 hrs by MTT assay	[PMID: 31398616]
RAW264.7	CC₅₀	> 100 μM Compound: 16	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 33667099]
RAW264.7	IC₅₀	31.2 μM Compound: 16	Inhibition of LPS induced NO production in mouse RAW264.7 cells measured after 24 hrs by Griess reagent based assay Inhibition of LPS induced NO production in mouse RAW264.7 cells measured after 24 hrs by Griess reagent based assay	[PMID: 33667099]
RAW264.7	IC₅₀	48.59 μM Compound: 1; Hes	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 2 hrs followed by LPS stimulation and measured after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 2 hrs followed by LPS stimulation and measured after 24 hrs by Griess method	[PMID: 33493826]
RPMI-8226	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
THP-1	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method	[PMID: 20192247]
U-266	EC₅₀	> 10 μM Compound: 3	Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
Vero	IC₅₀	8.3 μM Compound: Hesperetin	Inhibition of SARS coronavirus 3C-like protease cis-cleavage activity transfected in african green monkey Vero cells by luciferase reporter gene assay Inhibition of SARS coronavirus 3C-like protease cis-cleavage activity transfected in african green monkey Vero cells by luciferase reporter gene assay	[PMID: 23647823]

体外実験

Hesperetin (Compound HET) (100 μM) results in strong or moderate inhibition on 10 tested rhUGT isoforms^[2].
Hesperetin (100-800 μM, 24-48 h) significantly reduces the viabilities of U-251 and U-87 cells in a time- and concentration-dependent manner^[4].
Hesperetin (200-400 μM, 24 h) significantly increases the proportion of apoptotic cells in a dose-dependent manner in U-251 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[4]

Cell Line:	U-251 cell line
Concentration:	200 and 400 μM
Incubation Time:	24 h
Result:	Resulted in significant accumulation of cell populations at the G2/M phase, with a concomitant decrease of cell populations in G1 phase and a slightly elevated population in S phase.

Cell Viability Assay^[4]

Cell Line:	U-251 and U-87 cells
Concentration:	100, 200, 400, 600, and 800 μM
Incubation Time:	24 and 48 h
Result:	Significantly reduced the viabilities of U-251 and U-87 cells in a time- and concentration-dependent manner with IC₅₀s of 468.33 μM and 441.87 μM.

体内実験

Hesperetin (Compound Hp) (40 mg/kg, p.o., 21 d) reversed the activities of AChE to near normal in Cd-exposed rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Cd-exposed male albino Wistar rats (180-210 g)^[3]
Dosage:	40 mg/kg
Administration:	Oral gavage (p.o.)
Result:	Significantly reversed the activities of AChE to near normal. Decreased the levels of LPO products in brain. Significantly increased the levels of antioxidants in brain. Significantly increased the activities of ATPases enzymes in brain. Decreased the levels of Bax, cytochrome c, caspase 3 and 9 and increased the levels of Bcl2 markers. Significantly restores the activities of mitochondrial enzyme complex I, II, III and IV. Restored the levels of NO and OH^-. Restored the enzyme activities in cererbrum. Reduced the tail length, tail moment, and tail intensity.

臨床実験

分子量

302.28

分子式

C₁₆H₁₄O₆

CAS 番号

520-33-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1C[C@@H](C2=CC=C(OC)C(O)=C2)OC3=CC(O)=CC(O)=C13

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 125 mg/mL (413.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3082 mL	16.5410 mL	33.0819 mL
5 mM	0.6616 mL	3.3082 mL	6.6164 mL
10 mM	0.3308 mL	1.6541 mL	3.3082 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC/saline water
Solubility: 20 mg/mL (66.16 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.12%

Select Batch:

データシート (279 KB) SDS (393 KB)

COA (248 KB) LCMS (87 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Arya A, et al. Bioflavonoid hesperetin overcome bicalutamide induced toxicity by co-delivery in novel SNEDDS formulations: Optimization, in vivo evaluation and uptake mechanism. Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:954-964 [Content Brief]

[2]. Liu D, et al. Inhibitory Effect of Hesperetin and Naringenin on Human UDP-Glucuronosyltransferase Enzymes: Implications for Herb-Drug Interactions. Biol Pharm Bull. 2016;39(12):2052-2059. [Content Brief]

[3]. Shagirtha K, et al. Neuroprotective efficacy of hesperetin against cadmium induced oxidative stress in the brain of rats. Toxicol Ind Health. 2016 Nov 1. pii: 0748233716665301 [Content Brief]

[4]. Li Q, et al. Hesperetin Induces Apoptosis in Human Glioblastoma Cells via p38 MAPK Activation. Nutr Cancer. 2019 Jul 11:1-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3082 mL	16.5410 mL	33.0819 mL	82.7048 mL
	5 mM	0.6616 mL	3.3082 mL	6.6164 mL	16.5410 mL
	10 mM	0.3308 mL	1.6541 mL	3.3082 mL	8.2705 mL
	15 mM	0.2205 mL	1.1027 mL	2.2055 mL	5.5137 mL
	20 mM	0.1654 mL	0.8270 mL	1.6541 mL	4.1352 mL
	25 mM	0.1323 mL	0.6616 mL	1.3233 mL	3.3082 mL
	30 mM	0.1103 mL	0.5514 mL	1.1027 mL	2.7568 mL
	40 mM	0.0827 mL	0.4135 mL	0.8270 mL	2.0676 mL
	50 mM	0.0662 mL	0.3308 mL	0.6616 mL	1.6541 mL
	60 mM	0.0551 mL	0.2757 mL	0.5514 mL	1.3784 mL
	80 mM	0.0414 mL	0.2068 mL	0.4135 mL	1.0338 mL
	100 mM	0.0331 mL	0.1654 mL	0.3308 mL	0.8270 mL

Hesperetin Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hesperetin520-33-2p38 MAPKAutophagyApoptosisReactive Oxygen Species (ROS)NF-κBBcl-2 FamilyNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Hesperetin

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生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hesperetin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

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Hesperetin

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Hesperetin 関連抗体

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•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

p38 MAPK アイソフォーム固有の製品をすべて表示:

NF-κB アイソフォーム固有の製品をすべて表示:

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Hesperetin 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hesperetin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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