Cat. No.: HY-N0168A

(Rac)-Hesperetin

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(Rac)-Hesperetin is the racemate of Hesperetin (HY-N0168), an orally active multi-target inhibitor. (Rac)-Hesperetin exhibits significant anti-tumor and anti-inflammatory activities by blocking the TGF-β1-mediated Fyn/RhoA signaling axis and the TLR4-MyD88-NF-κB inflammatory pathway. (Rac)-Hesperetin inhibits the formation of actin stress fibers and the migration and invasion of cancer cells, and is suitable for triple-negative breast cancer research. In inflammation models, (Rac)-Hesperetin effectively alleviates lung injury by reducing the release of pro-inflammatory mediators and regulating the activity of oxidative stress enzymes, and is suitable for acute lung injury research. (Rac)-Hesperetin also interferes with the entry and early replication processes of channel catfish virus, inhibits viral gene expression and progeny virus production, thereby protecting cells from virus-induced cytopathic effects.

For research use only. We do not sell to patients.

Purity: 99.28%
CAS No.: 69097-99-0
Formula: C₁₆H₁₄O₆
Molecular Weight:302.28

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

(Rac)-Hesperetin Chemical Structure

CAS No.: 69097-99-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All TGF-beta/Smad Isoforms :

Smad1
Smad3

All NF-κB Isoforms :

In Vitro

Hesperetin (25, 50, 100 μM; 0, 24, 48, 72 h) significantly inhibits the migratory capacity of TGF-β1-induced human triple-negative breast cancer MDA-MB-231 cells in a dose-dependent manner^[1].
Hesperetin (500 μg/mL; 36 h) significantly inhibits the expression of ORF39 and ORF59 proteins of CCV, completely alleviates the cytopathic effect on CCO cells induced by CCV infection, and exerts a cytoprotective effect^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Human triple-negative breast cancer MDA-MB-231 cells
Concentration:

25, 50, 100 μM
Incubation Time:

24, 48, 72 h
Result:

Significantly inhibited TGF-β1-induced migration of MDA-MB-231 cells in a dose-dependent manner at 24 h, 48 h and 72 h after treatment.

In Vivo

Hesperetin (10-30 mg/kg; intragastric administration; single dose; administered 1 h after lipopolysaccharide modeling) significantly reduces the levels of IL-6 and TNF-α as well as the number of inflammatory cells in bronchoalveolar lavage fluid, alleviates lung tissue injury, decreases the lung wet/dry weight ratio, downregulates the activities of myeloperoxidase and lactate dehydrogenase, and enhances the activity of superoxide dismutase in a lipopolysaccharide-induced acute lung injury model of male C57 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57 male mice (8 weeks old, 18-22 g) with LPS-induced acute lung injury^[3]
Dosage:

Hesperetin: 10 mg/kg, 20 mg/kg , 30 mg/kg ;
Positive control: 2 mg/kg Dexamethasone (oral gavage)
Administration:

oral gavage
Result:

At 1 h after LPS intratracheal instillation, significantly suppressed the levels of interleukin-6 and tumor necrosis factor-α in bronchoalveolar lavage fluid, reduced the number of inflammatory cells in bronchoalveolar lavage fluid, ameliorated LPS-induced histopathologic changes of lung tissue and decreased lung inflammatory scores.
Reduced the lung wet/dry weight ratio, lowered the activities of myeloperoxidase and lactate dehydrogenase in lung tissue, enhanced superoxide dismutase activity, and downregulated the protein expression of Toll-like receptor 4 and myeloid differentiation factor 88 in lung tissue.
Suppressed the phosphorylation of nuclear factor-κB p65, IκB-α and TAK1, and inhibited the activation of nuclear factor-κB pathway in lung tissue, with the 30 mg/kg dosage showing the most significant therapeutic effect.

Chemical Information

CAS No. 69097-99-0
Appearance Solid
Molecular Weight 302.28
Formula C₁₆H₁₄O₆
Color White to yellow
SMILES

O=C1CC(C2=CC=C(OC)C(O)=C2)OC3=CC(O)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (413.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3082 mL

16.5410 mL

33.0819 mL

5 mM

0.6616 mL

3.3082 mL

6.6164 mL

10 mM

0.3308 mL

1.6541 mL

3.3082 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lu Q, et al. Hesperetin Inhibits TGF-β1-Induced Migration and Invasion of Triple Negative Breast Cancer MDA-MB-231 Cells via Suppressing Fyn/Paxillin/RhoA Pathway. Integr Cancer Ther. 2022;21:15347354221086900. [Content Brief]

[2]. Wang Y, et al. Hesperetin as a potent antiviral agent against channel catfish virus by interfering with viral internalization and early replication[J]. Aquaculture Reports, 2025, 45: 103228.

[3]. Wang N, et al. Hesperetin ameliorates lipopolysaccharide-induced acute lung injury in mice through regulating the TLR4-MyD88-NF-κB signaling pathway. Arch Pharm Res. 2019;42(12):1063-1070. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3082 mL	16.5410 mL	33.0819 mL	82.7048 mL
	5 mM	0.6616 mL	3.3082 mL	6.6164 mL	16.5410 mL
	10 mM	0.3308 mL	1.6541 mL	3.3082 mL	8.2705 mL
	15 mM	0.2205 mL	1.1027 mL	2.2055 mL	5.5137 mL
	20 mM	0.1654 mL	0.8270 mL	1.6541 mL	4.1352 mL
	25 mM	0.1323 mL	0.6616 mL	1.3233 mL	3.3082 mL
	30 mM	0.1103 mL	0.5514 mL	1.1027 mL	2.7568 mL
	40 mM	0.0827 mL	0.4135 mL	0.8270 mL	2.0676 mL
	50 mM	0.0662 mL	0.3308 mL	0.6616 mL	1.6541 mL
	60 mM	0.0551 mL	0.2757 mL	0.5514 mL	1.3784 mL
	80 mM	0.0414 mL	0.2068 mL	0.4135 mL	1.0338 mL
	100 mM	0.0331 mL	0.1654 mL	0.3308 mL	0.8270 mL

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(Rac)-Hesperetin

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All TGF-beta/Smad Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(Rac)-Hesperetin Related Classifications

HY-N0168A Related Classifications