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  5. Hygromycin B

Hygromycin B  (Synonyms: Hygrovetine)

Art. -Nr.: HY-B0490 Reinheit: 99.93%
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Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr. : 31282-04-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 37 publication(s) in Google Scholar

Other Forms of Hygromycin B:

Hygromycin B Related Antibodies

Top Publications Citing Use of Products

37 Publications Citing Use of MCE Hygromycin B

Cell Proliferation/Viability Assay
WB
IF

    Hygromycin B purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 Jun 23;60(12):1751-1767.e9.  [Abstract]

    Cell viability assay of LN229, SNB-19, MES50, and MES52 cells treated with different concentrations of HB (Hygromycin B) (5-20 μM) for 24h. Data were shown as mean ± SD (n = 3).

    Hygromycin B purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 Jun 23;60(12):1751-1767.e9.  [Abstract]

    Effect of HB (Hygromycin B) treatment (10 μM) for 24h on the levels of p-STAT3, STAT3, CD44, and SPP1 in MES50 cells transfected with Vector or CHI3L1.

    Hygromycin B purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 Jun 23;60(12):1751-1767.e9.  [Abstract]

    IF analysis of the subcellular localization of p-STAT3, GAPDH, CD44, and SPP1 in MES50 cells after pretreatment with CHI3L1WT along, HB (Hygromycin B) along, or CHI3L1WT+HB. DAPI was used to visualize the nuclei (blue). Scale bars, 40 μm.

    Alle Antibiotic Isoform-spezifische Produkte anzeigen:

    Alle Isoformen anzeigen
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    Alle Parasite Isoform-spezifische Produkte anzeigen:

    Alle Isoformen anzeigen
    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

    • Biologische Aktivität

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    Beschreibung

    Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

    IC50 & Target

    Aminoglycoside

     

    In Vitro

    Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1].
    Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Hygromycin B Related Antibodies
    In Vivo

    Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3].
    Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molekulargewicht

    527.52

    Formel

    C20H37N3O13
    CAS. Nr.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)([H])[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)([H])[C@@H]([C@H]4NC)O)([H])[C@@]([C@H]3O)([H])O2
    Structure Classification
    Initial Source

    Escherichia coli
    Versand

    Room temperature in continental US; may vary elsewhere.
    Speicherung
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Lösungsmittel & Löslichkeit
    In Vitro: 

    H2O : 125 mg/mL (236.96 mM; Need ultrasonic)

    DMSO : 66.67 mg/mL (126.38 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8957 mL 9.4783 mL 18.9566 mL
    5 mM 0.3791 mL 1.8957 mL 3.7913 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molaritätsrechner

    • Verdünnungsrechner

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (9.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (9.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (189.57 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Reinheit & Dokumentation

    Purity: 99.93%

    SDS (480 KB)

    COA (256 KB) HNMR (303 KB) RP-HPLC (223 KB)

    Handling Instructions (2659 KB)
    Verweise
    Tierverwaltung
    [4]

    Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4].

    MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
    Verweise

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.8957 mL 9.4783 mL 18.9566 mL 47.3916 mL
    5 mM 0.3791 mL 1.8957 mL 3.7913 mL 9.4783 mL
    10 mM 0.1896 mL 0.9478 mL 1.8957 mL 4.7392 mL
    15 mM 0.1264 mL 0.6319 mL 1.2638 mL 3.1594 mL
    20 mM 0.0948 mL 0.4739 mL 0.9478 mL 2.3696 mL
    25 mM 0.0758 mL 0.3791 mL 0.7583 mL 1.8957 mL
    30 mM 0.0632 mL 0.3159 mL 0.6319 mL 1.5797 mL
    40 mM 0.0474 mL 0.2370 mL 0.4739 mL 1.1848 mL
    50 mM 0.0379 mL 0.1896 mL 0.3791 mL 0.9478 mL
    60 mM 0.0316 mL 0.1580 mL 0.3159 mL 0.7899 mL
    80 mM 0.0237 mL 0.1185 mL 0.2370 mL 0.5924 mL
    100 mM 0.0190 mL 0.0948 mL 0.1896 mL 0.4739 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Hygromycin B Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hygromycin B31282-04-9HygrovetineBacterialFungalAntibioticParasiteInhibitorinhibitorinhibit

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