1. PI3K/Akt/mTOR
    Stem Cell/Wnt
    MAPK/ERK Pathway
  2. Akt
    ERK

Pachymic acid (Synonyms: 3-O-Acetyltumulosic acid)

Cat. No.: HY-N0371
Handling Instructions

Pachymic acid is a lanostrane-type triterpenoid from P. cocos. Pachymic acid inhibits Akt and ERK signaling pathways.

For research use only. We do not sell to patients.

Pachymic acid Chemical Structure

Pachymic acid Chemical Structure

CAS No. : 29070-92-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 251 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
5 mg USD 216 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
10 mg USD 336 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
50 mg USD 1056 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • References

Description

Pachymic acid is a lanostrane-type triterpenoid from P. cocos. Pachymic acid inhibits Akt and ERK signaling pathways.

IC50 & Target[1]

Akt

 

ERK

 

In Vitro

Pachymic acid (PA) is able to inhibit gallbladder cancer tumorigenesis involving affection of Akt and ERK signaling pathways. Pachymic acid (PA) treatment significantly inhibits Rho A, Akt and ERK pathway in gallbladder carcinoma cells. Pachymic acid (PA) treatment can dose-dependently downregulate PCNA, ICAM-1, RhoA, p-Akt and pERK. Cell growth is inhibited by 10 µg/mL Pachymic acid (PA) 12 h after treatment, and a concentration of 30 µg/mL further reduced cell growth. The growth of cells is suppressed in a time- and dose-dependent manner. After 48 h treatment, about 25%, 40% and 70% of the cell growth are inhibited by Pachymic acid (PA) at concentration of 10 µg/mL, 20 µg/mL and 30 µg/mL, respectively. Pachymic acid (PA) also inhibits the growth of gallbladder carcinoma cells in a time-dependent and dose-dependent manner[1].

In Vivo

To evaluate the anti-tumor activity of Pachymic acid (PA) in vivo, human lung cancer NCI-H23 tumor xenograft models are used. Pachymic acid (PA) significantly suppresses tumor growth at doses of 30 and 60 mg/kg for 21 days compared with the control group[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.8912 mL 9.4561 mL 18.9122 mL
5 mM 0.3782 mL 1.8912 mL 3.7824 mL
10 mM 0.1891 mL 0.9456 mL 1.8912 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Pachymic acid (PA) is reconstituted in DMSO at a stock concentration of 20 mM and subsequently diluted tothe working concentrations for experimental procedures. Control treatments contained an equivalent concentration of DMSO (0.1%) for all experiments[1].

The antiproliferative effect of Pachymic acid (PA) on GBC-SD cells is evaluated using a cell counting kit-8 (CCK-8). Briefly, after indicated treatment, 10 µL of CCK-8 solution is added into each well, and following one hincubation, absorbance is measured at 450 nm using a microplate reader[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Pachymic acid (PA) is prepared in 0.1% DMSO in physiological saline (Mice)[2].

Mice[2]
Female athymic nude mice of 4-5 weeks of age are used. Exponentially growing NCI-H23 cells (5×106 in 100 µL PBS) are injected subcutaneously in the right flank of each mouse. Tumor xenografts are allowed to grow to an average size of 100-200 mm3 and are randomly assigned to four different treatment groups (six mice per group): (a) vehicle control (0.1% DMSO in physiological saline); (b) Pachymic acid (PA) 10 mg/kg; (c) PA 30 mg/kg; (Dd) PA 60 mg/kg. The mice are administered PA via intraperitoneal (ip) injections for 3 weeks (5 days/week). Tumor size is measured on two axes with the aid of Vernier calipers and tumor volume (mm3) is calculated.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

528.76

Formula

C₃₃H₅₂O₅

CAS No.

29070-92-6

SMILES

#593a8r9+R/ZMjoV+F/kOjoVjmFYnFCAjmFW+gowKa8wKUC9+go9KROY+gSs+gf9 nFCA+mFWnFCAMaRW+gf9+iZYKROY+iOs+iQV+F/kCEo9KTr9+mFW+FOiKjoVjmFY +go9Kjo9KTr9+R/ZMjow+go9Ka8wKUC9CA==

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Pachymic acid
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HY-N0371
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