Cat. No.: HY-N0371

Pachymic acid

(Synonyms: 3-O-Acetyltumulosic acid)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Pachymic acid is a lanostrane-type triterpenoid from P. cocos. Pachymic acid inhibits Akt and ERK signaling pathways.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 29070-92-6
Formula: C₃₃H₅₂O₅
Molecular Weight:528.76

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Pachymic acid Chemical Structure

CAS No.: 29070-92-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Pachymic acid is a lanostrane-type triterpenoid from P. cocos. Pachymic acid inhibits Akt and ERK signaling pathways.

IC₅₀ & Target^[1]

ERK

All Akt Isoforms :

All ERK Isoforms :

In Vitro

Pachymic acid (PA) is able to inhibit gallbladder cancer tumorigenesis involving affection of Akt and ERK signaling pathways. Pachymic acid (PA) treatment significantly inhibits Rho A, Akt and ERK pathway in gallbladder carcinoma cells. Pachymic acid (PA) treatment can dose-dependently downregulate PCNA, ICAM-1, RhoA, p-Akt and pERK. Cell growth is inhibited by 10 μg/mL Pachymic acid (PA) 12 h after treatment, and a concentration of 30 μg/mL further reduced cell growth. The growth of cells is suppressed in a time- and dose-dependent manner. After 48 h treatment, about 25%, 40% and 70% of the cell growth are inhibited by Pachymic acid (PA) at concentration of 10 μg/mL, 20 μg/mL and 30 μg/mL, respectively. Pachymic acid (PA) also inhibits the growth of gallbladder carcinoma cells in a time-dependent and dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

To evaluate the anti-tumor activity of Pachymic acid (PA) in vivo, human lung cancer NCI-H23 tumor xenograft models are used. Pachymic acid (PA) significantly suppresses tumor growth at doses of 30 and 60 mg/kg for 21 days compared with the control group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 29070-92-6
Appearance Solid
Molecular Weight 528.76
Formula C₃₃H₅₂O₅
Color White to off-white
SMILES

O[C@@H]([C@]1([H])[C@H](C(O)=O)CCC(C(C)C)=C)C[C@@]([C@]1(C)CC2)(C)C3=C2[C@]4(C)[C@](CC3)([H])C(C)(C)[C@@H](OC(C)=O)CC4
Synonyms

3-O-Acetyltumulosic acid
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

All Histological Imaging/StainingCell Proliferation/Viability AssayWBRT-PCR

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Journal Impact Factor

Chem Biol Interact

Pachymic acid suppresses proliferation and invasion in colon cancer by inhibiting glycolysis via PPARγ/ENO1 pathway. [Abstract]

2026 Jan 25:424:111881. PMID: 41390134
Mol Med Rep

Effects and molecular mechanism of pachymic acid on ferroptosis in renal ischemia reperfusion injury. [Abstract]

2021 Jan;23(1):63. PMID: 33215224

Pachymic acid purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2021 Jan;23(1):63. [Abstract]

Hematoxylin and eosin images of the kidneys from mice in the Sham, IRI, IRI+Pachymic acid (PA_L) (5 mg/kg), IRI+PA_M (10 mg/kg) and IRI+PA_H (20 mg/kg) group.

Pachymic acid purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2021 Jan;23(1):63. [Abstract]

Pachymic acid (PA) (5, 10, 20 mg/kg, i.p.) increases the expression levels the ferroptosis related proteins, GPX4, xCT and HO-1 by Western blotting.

Pachymic acid purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2021 Jan;23(1):63. [Abstract]

Reverse transcription-quantitative PCR analysis was performed to detect the mRNA expression levels of the ferroptosis related proteins treated with Pachymic acid (PA) (5, 10, 20 mg/kg, i.p.).
Biol Pharm Bull

Pachymic acid inhibits growth and metastatic potential in liver cancer HepG2 and Huh7 cells. [Abstract]

2023 Jan 1;46(1):35-41. PMID: 36273899
J Int Med Res

Pachymic acid alleviates lipopolysaccharide-induced cystitis glandularis by inhibiting ferroptosis. [Abstract]

2025 Nov;53(11):3000605251396755. PMID: 41299196

Pachymic acid purchased from MedChemExpress. Usage Cited in: J Int Med Res. 2025 Nov;53(11):3000605251396755. [Abstract]

After treating the cells with different concentrations of Pachymic acid (PA) (0, 20, 40, 60, and 80 μg/mL) for 24 h, cell viability was measured using the CCK8 assay.

Pachymic acid purchased from MedChemExpress. Usage Cited in: J Int Med Res. 2025 Nov;53(11):3000605251396755. [Abstract]

Histopathological sections of bladder tissues from each group were observed via H&E staining treated with Pachymic acid (PA) (40 mg/kg, i.p.).
Biosci Biotechnol Biochem

Pachymic acid alleviates experimental pancreatic fibrosis through repressing NLRP3 inflammasome activation. [Abstract]

2022 Oct 20;86(11):1497-1505. PMID: 36095138

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (9.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 4.17 mg/mL (7.89 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8912 mL

9.4561 mL

18.9122 mL

5 mM

0.3782 mL

1.8912 mL

3.7824 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 1 mg/mL (1.89 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 1 mg/mL (1.89 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (1.89 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

The antiproliferative effect of Pachymic acid (PA) on GBC-SD cells is evaluated using a cell counting kit-8 (CCK-8). Briefly, after indicated treatment, 10 µL of CCK-8 solution is added into each well, and following one hincubation, absorbance is measured at 450 nm using a microplate reader^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Mice^[2]
Female athymic nude mice of 4-5 weeks of age are used. Exponentially growing NCI-H23 cells (5×10⁶ in 100 µL PBS) are injected subcutaneously in the right flank of each mouse. Tumor xenografts are allowed to grow to an average size of 100-200 mm³ and are randomly assigned to four different treatment groups (six mice per group): (a) vehicle control (0.1% DMSO in physiological saline); (b) Pachymic acid (PA) 10 mg/kg; (c) PA 30 mg/kg; (Dd) PA 60 mg/kg. The mice are administered PA via intraperitoneal (ip) injections for 3 weeks (5 days/week). Tumor size is measured on two axes with the aid of Vernier calipers and tumor volume (mm³) is calculated.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen Y, et al. Pachymic acid inhibits tumorigenesis in gallbladder carcinoma cells. Int J Clin Exp Med. 2015 Oct 15;8(10):17781-8. [Content Brief]

[2]. Ma J, et al. Pachymic acid induces apoptosis via activating ROS-dependent JNK and ER stress pathways in lung cancer cells. Cancer Cell Int. 2015 Aug 5;15:78. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	1.8912 mL	9.4561 mL	18.9122 mL	47.2804 mL
Ethanol / DMSO	5 mM	0.3782 mL	1.8912 mL	3.7824 mL	9.4561 mL

Keywords

Pachymic acid

5 Cited Publications

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Pachymic acid

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Publications Citing Use of MedChemExpress (MCE) Pachymic acid

All Akt Isoforms

All ERK Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Pachymic acid Related Classifications

HY-N0371 Related Classifications