1. Immunology/Inflammation
    Metabolic Enzyme/Protease
    Anti-infection
  2. Toll-like Receptor (TLR)
    MMP
    HSV
    Antibiotic
  3. FSL-1 TFA

FSL-1 TFA 

Cat. No.: HY-P2036A Purity: 99.58%
Handling Instructions

FSL-1 TFA, a bacterial-derived toll-like receptor 2/6 (TLR2/6) agonist, enhances resistance to experimental HSV-2 infection. FSL-1 TFA induces MMP-9 production through TLR2 and NF-κB/AP-1 signaling pathways in monocytic THP-1 cells.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

FSL-1 TFA Chemical Structure

FSL-1 TFA Chemical Structure

Size Price Stock Quantity
100 μg USD 160 In-stock
Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

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Description

FSL-1 TFA, a bacterial-derived toll-like receptor 2/6 (TLR2/6) agonist, enhances resistance to experimental HSV-2 infection[1]. FSL-1 TFA induces MMP-9 production through TLR2 and NF-κB/AP-1 signaling pathways in monocytic THP-1 cells[2].

IC50 & Target[1][2]

TLR2

 

TLR6

 

MMP-9

 

In Vitro

FSL-1 significantly reduces HSV-2 replication in human vaginal epithelial cells (EC)[1].
FSL-1 induces significant resistance to experimental genital HSV-2 infection through elaboration of a specific cytokine response profile[1].
FSL-1 (50 ng/mL, 24 hours) induces MMP-9 expression at both mRNA and protein levels in human monocytic THP-1 cells[2].
FSL-1 activates the MAP kinase/NF-κB signaling pathway[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: V11I, V12I or V19I immortalized human vaginal EC
Concentration: 6 μg or 0.1 μg
Incubation Time: Added at 24, 6 or just prior to HSV-2 inoculation (104pfu/well)
Result: The 6 μg does produced significant reductions when delivered at 24 or 6 h prior to HSV-2 inoculation. The 0.1 μg dose produced reduced HSV-2 replication at 24 or 6 h prior to viral challenge.
In Vivo

FSL-1 application significantly protectes against genital HSV-2 challenge in mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Swiss-Webster mice (weighing 20-25 g)[1]
Dosage: 2 or 6 μg
Administration: Delivered vaginally using a positive displacement pipet, prior to or following viral challenge as specified for each experiment.
Result: The 2 μg does delivered 6 h prior to HSV-2 challenge increased the ID50 (260 pfu) and LD50 (660 pfu) by 10-fold compared to DPBS vehicle control.
The single 6 μg dose produced significantly improved outcomes compared to DPBS vehicle application.
Molecular Weight

1780.18

Formula

C₈₂H₁₄₁F₃N₁₄O₂₀S

Sequence

S-(2, 3-Bispalmitoyloxypropyl)-Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe

Sequence Shortening

S-(2, 3-Bispalmitoyloxypropyl)-CGDPKHPKSF

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Protect from light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

FSL-1FSL1FSL 1Toll-like Receptor (TLR)MMPHSVAntibioticMatrix metalloproteinasesHerpes simplex virusInhibitorinhibitorinhibit

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FSL-1 TFA
Cat. No.:
HY-P2036A
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