5-Hydroxytryptophan (Synonyms: 5-HTP; DL-5-Hydroxytryptophan)

Name: 5-Hydroxytryptophan
Brand: MedChemExpress
SKU: HY-N0122
Rating: 5.0 (2 reviews)

Cat. No.: HY-N0122 Purity: 99.89%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

5-Hydroxytryptophan, a tryptophan metabolite, is a direct 5-hydroxytryptamine (5-HT) precursor and an L-aromatic amino acid decarboxylase substrate. .

For research use only. We do not sell to patients.

CAS No. : 56-69-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of 5-Hydroxytryptophan:

5-Hydroxytryptophan (Standard) Get quote

2 Publications Citing Use of MCE 5-Hydroxytryptophan

RT-PCR

Bio/Physico-chemical Assay

: 5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]; Relative IDO1 transcription levels detected by quantitative reverse transcription PCR (qRT-PCR) analysis and IDO1 levels detected by western blot in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-Hydroxytryptophan (5-HTP; 500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.

: 5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]; Relative IDO1 transcription levels detected by qRT-PCR analysis and IDO1 levels detected by western blot in BT549 cells treated with 5-Hydroxytryptophan (5-HTP; 0, 0.25, 0.5, 1, 2 μM) at different concentrations for 24 hours (Left) and with 5-HTP (500 µM) for different durations (0, 6, 12, 24, 48 h) (Right).

: 5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]; BT549 cells treated with phosphate buffer saline (PBS) or 5-Hydroxytryptophan (5-HTP; 500 µM) for 24 hours.

: 5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]; Representative immunofluorescence images of AHR and the cytoplasmic and nuclear protein fractions of the AHR protein in BT549 cells treated with phosphate buffer saline (PBS) or 5-HTP (500 µM) for 24 hours.

: 5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]; Chromatin immunoprecipitation followed by quantitative PCR analysis of BT549 cells treated with 5-Hydroxytryptophan (5-HTP; 1 mM) for 48 hours.

: 5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]; Analysis of luciferase activity in human embryonic kidney (HEK) 293 T cells. HEK-293 T cells concomitantly expressing an IDO1 promoter–luciferase reporter and AHR were treated with 5-Hydroxytryptophan (5-HTP; 500 µM) or PBS for 24 hours.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

View All 5-HT Receptor Isoform Specific Products:

View All Isoforms

5-HT Receptor 5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Hydroxytryptophan, a tryptophan metabolite, is a direct 5-hydroxytryptamine (5-HT) precursor and an L-aromatic amino acid decarboxylase substrate. ^[1]^[2]^[3].

IC₅₀ & Target

Human Endogenous Metabolite

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

5-Hydroxytryptophan (50-100 mg/kg, ip, single dose) increases the synthesis and release of 5-HT in the brain, thus activates 5-HT2 receptor and promotes the secretion of prolactin and ACTH in rats models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley rats^[1]
Dosage:	50-100 mg/kg
Administration:	ip, single dose
Result:	Increased the secretion of prolactin and ACTH.

Clinical Trial

Molecular Weight

220.23

Formula

C₁₁H₁₂N₂O₃

CAS No.

56-69-9

Appearance

Solid

Color

White to off-white

SMILES

NC(CC1=CNC2=CC=C(O)C=C12)C(O)=O

Structure Classification

Amino acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (378.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 12.5 mg/mL (56.76 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5407 mL	22.7037 mL	45.4075 mL
5 mM	0.9081 mL	4.5407 mL	9.0815 mL
10 mM	0.4541 mL	2.2704 mL	4.5407 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.08 mg/mL (9.44 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.08 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 5.88 mg/mL (26.70 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 20 mg/mL (90.81 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (276 KB) SDS (479 KB)

COA (250 KB) RP-HPLC (262 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Gartside SE, et al. Mediation of ACTH and prolactin responses to 5-HTP by 5-HT2 receptors. Eur J Pharmacol. 1990 Apr 10;179(1-2):103-9. [Content Brief]

[2]. Gijsman HJ, et al. Placebo-controlled comparison of three dose-regimens of 5-hydroxytryptophan challenge test in healthy volunteers. J Clin Psychopharmacol. 2002 Apr;22(2):183-9. [Content Brief]

[3]. Schruers K, et al. Acute L-5-hydroxytryptophan administration inhibits carbon dioxide-induced panic in panic disorder patients. Psychiatry Res. 2002 Dec 30;113(3):237-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.5407 mL	22.7037 mL	45.4075 mL	113.5187 mL
	5 mM	0.9081 mL	4.5407 mL	9.0815 mL	22.7037 mL
	10 mM	0.4541 mL	2.2704 mL	4.5407 mL	11.3519 mL
	15 mM	0.3027 mL	1.5136 mL	3.0272 mL	7.5679 mL
	20 mM	0.2270 mL	1.1352 mL	2.2704 mL	5.6759 mL
	25 mM	0.1816 mL	0.9081 mL	1.8163 mL	4.5407 mL
	30 mM	0.1514 mL	0.7568 mL	1.5136 mL	3.7840 mL
	40 mM	0.1135 mL	0.5676 mL	1.1352 mL	2.8380 mL
	50 mM	0.0908 mL	0.4541 mL	0.9081 mL	2.2704 mL
DMSO	60 mM	0.0757 mL	0.3784 mL	0.7568 mL	1.8920 mL
	80 mM	0.0568 mL	0.2838 mL	0.5676 mL	1.4190 mL
	100 mM	0.0454 mL	0.2270 mL	0.4541 mL	1.1352 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

5-Hydroxytryptophan Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Hydroxytryptophan56-69-95-HTP DL-5-HydroxytryptophanEndogenous Metabolite5-HT ReceptorSerotonin Receptor5-hydroxytryptamine ReceptorInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

5-Hydroxytryptophan (Synonyms: 5-HTP; DL-5-Hydroxytryptophan)

Customer Review

Other Forms of 5-Hydroxytryptophan:

5-Hydroxytryptophan Related Antibodies

2 Publications Citing Use of MCE 5-Hydroxytryptophan

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All 5-HT Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

5-Hydroxytryptophan Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report