2. 疾患モデリングル製品 Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein
  3. Nervous System Disease Models Endogenous Metabolite 5-HT Receptor
  4. Depression Models
  5. 5-Hydroxytryptophan

5-Hydroxytryptophan  (Synonyms: 5-HTP; DL-5-Hydroxytryptophan)

製品番号: HY-N0122 純度: 99.89%
COA 取扱説明書 Technical Support

5-Hydroxytryptophan, a tryptophan metabolite, is a direct 5-hydroxytryptamine (5-HT) precursor and an L-aromatic amino acid decarboxylase substrate. .

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CAS 番号 : 56-69-9

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 38 在庫あり
Solution
10 mM * 1 mL in DMSO USD 38 在庫あり
Solid
5 mg $35 在庫あり
10 mg $55 在庫あり
25 mg $100 在庫あり
50 mg $165 在庫あり
100 mg $237 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of 5-Hydroxytryptophan:

5-Hydroxytryptophan 関連抗体

Top Publications Citing Use of Products

    5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Relative IDO1 transcription levels detected by quantitative reverse transcription PCR (qRT-PCR) analysis and IDO1 levels detected by western blot in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-Hydroxytryptophan (5-HTP; 500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.

    5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Relative IDO1 transcription levels detected by qRT-PCR analysis and IDO1 levels detected by western blot in BT549 cells treated with 5-Hydroxytryptophan (5-HTP; 0, 0.25, 0.5, 1, 2 μM) at different concentrations for 24 hours (Left) and with 5-HTP (500 µM) for different durations (0, 6, 12, 24, 48 h) (Right).

    5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    BT549 cells treated with phosphate buffer saline (PBS) or 5-Hydroxytryptophan (5-HTP; 500 µM) for 24 hours.

    5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Representative immunofluorescence images of AHR and the cytoplasmic and nuclear protein fractions of the AHR protein in BT549 cells treated with phosphate buffer saline (PBS) or 5-HTP (500 µM) for 24 hours.

    5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Chromatin immunoprecipitation followed by quantitative PCR analysis of BT549 cells treated with 5-Hydroxytryptophan (5-HTP; 1 mM) for 48 hours.

    5-Hydroxytryptophan purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Analysis of luciferase activity in human embryonic kidney (HEK) 293 T cells. HEK-293 T cells concomitantly expressing an IDO1 promoter–luciferase reporter and AHR were treated with 5-Hydroxytryptophan (5-HTP; 500 µM) or PBS for 24 hours.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    5-HT Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    5-HT Receptor 5-HT1 Receptor 5-HT2 Receptor 5-HT3 Receptor 5-HT4 Receptor 5-HT5 Receptor 5-HT6 Receptor 5-HT7 Receptor

    • 生物活性

    • 純度とドキュメンテーション

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    製品説明

    5-Hydroxytryptophan, a tryptophan metabolite, is a direct 5-hydroxytryptamine (5-HT) precursor and an L-aromatic amino acid decarboxylase substrate. [1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    体内実験

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    5-Hydroxytryptophan (50-100 mg/kg, ip, single dose) increases the synthesis and release of 5-HT in the brain, thus activates 5-HT2 receptor and promotes the secretion of prolactin and ACTH in rats models[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Sprague-Dawley rats[1]
    Dosage: 50-100 mg/kg
    Administration: ip, single dose
    Result: Increased the secretion of prolactin and ACTH.
    臨床実験
    分子量

    220.23

    分子式

    C11H12N2O3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(CC1=CNC2=CC=C(O)C=C12)C(O)=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 83.33 mg/mL (378.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 12.5 mg/mL (56.76 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5407 mL 22.7037 mL 45.4075 mL
    5 mM 0.9081 mL 4.5407 mL 9.0815 mL
    10 mM 0.4541 mL 2.2704 mL 4.5407 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 5.88 mg/mL (26.70 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    • Protocol 2

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 20 mg/mL (90.81 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.92%

    COA (250 KB) RP-HPLC (262 KB) LCMS (87 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.5407 mL 22.7037 mL 45.4075 mL 113.5187 mL
    5 mM 0.9081 mL 4.5407 mL 9.0815 mL 22.7037 mL
    10 mM 0.4541 mL 2.2704 mL 4.5407 mL 11.3519 mL
    15 mM 0.3027 mL 1.5136 mL 3.0272 mL 7.5679 mL
    20 mM 0.2270 mL 1.1352 mL 2.2704 mL 5.6759 mL
    25 mM 0.1816 mL 0.9081 mL 1.8163 mL 4.5407 mL
    30 mM 0.1514 mL 0.7568 mL 1.5136 mL 3.7840 mL
    40 mM 0.1135 mL 0.5676 mL 1.1352 mL 2.8380 mL
    50 mM 0.0908 mL 0.4541 mL 0.9081 mL 2.2704 mL
    DMSO 60 mM 0.0757 mL 0.3784 mL 0.7568 mL 1.8920 mL
    80 mM 0.0568 mL 0.2838 mL 0.5676 mL 1.4190 mL
    100 mM 0.0454 mL 0.2270 mL 0.4541 mL 1.1352 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    5-Hydroxytryptophan Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5-Hydroxytryptophan56-69-95-HTP DL-5-HydroxytryptophanEndogenous Metabolite5-HT ReceptorSerotonin Receptor5-hydroxytryptamine ReceptorInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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