1. PI3K/Akt/mTOR Anti-infection Apoptosis Autophagy
  2. mTOR Influenza Virus Apoptosis Autophagy
  3. Aloe emodin

Aloe emodin  (Synonyms: Rhabarberone; 3-Hydroxymethylchrysazine)

Cat. No.: HY-N0189 Pureté: 98.32%
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Aloe emodin (Rhabarberone) is a natural hydroxyanthraquinone with antitumor activities. aloe-emodin can bind with mTORC2 and inhibit its kinase activity. Aloe emodin exerts antiproliferation effects and induces cellular apoptosis. Aloe emodin also exhibits antiviral activity that against influenza A virus.

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CAS No. : 481-72-1

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10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO En stock
Solid
100 mg En stock
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Based on 3 publication(s) in Google Scholar

Other Forms of Aloe emodin:

Top Publications Citing Use of Products
In Vivo Efficacy Study
Histological Imaging/Staining
ELISA

    Aloe emodin purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jul 22;10(15):e34932.  [Abstract]

    Changes in body weights of mice were monitored following treatment with different doses of AE (Aloe emodin) in a mouse model of colitis induced by DSS.

    Aloe emodin purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jul 22;10(15):e34932.  [Abstract]

    Aloe emodin (5,10,50 mg/mL). H&E staining of colon tissue sections. Histological evaluation of H&E-stained colon tissue sections to assess tissue damage and inflammation.

    Aloe emodin purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jul 22;10(15):e34932.  [Abstract]

    Aloe emodin (5, 10, 50 mg/kg). Serum levels of inflammatory cytokines IL-17 and TNF-α were detected by ELISA.

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    Description

    Aloe emodin (Rhabarberone) is a natural hydroxyanthraquinone with antitumor activities. aloe-emodin can bind with mTORC2 and inhibit its kinase activity. Aloe emodin exerts antiproliferation effects and induces cellular apoptosis[1]. Aloe emodin also exhibits antiviral activity that against influenza A virus[2].

    IC50 & Target[1]

    mTORC2

     

    Cellular Effect
    Cell Line Type Value Description References
    A2780 IC50
    26 3
    Compound: 2
    Cytotoxicity against human A2780 cells
    Cytotoxicity against human A2780 cells
    [PMID: 24326280]
    B16-F10 IC50
    3.12 3
    Compound: 1
    Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    A2780 IC50
    26 3
    Compound: 2
    Cytotoxicity against human A2780 cells
    Cytotoxicity against human A2780 cells
    [PMID: 24326280]
    B16-F10 IC50
    3.12 3
    Compound: 1
    Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HCT-116 IC50
    8.7 3
    Compound: 4
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    [PMID: 17949858]
    B16-F10 IC50
    3.12 3
    Compound: 1
    Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HCT-116 IC50
    8.7 3
    Compound: 4
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    [PMID: 17949858]
    HEK293 IC50
    1 3
    Compound: 1
    Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HEK293 IC50
    1 3
    Compound: 1
    Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HepG2 IC50
    10 3
    Compound: 4
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    [PMID: 17949858]
    HCT-116 IC50
    8.7 3
    Compound: 4
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    [PMID: 17949858]
    HepG2 IC50
    6.13 3
    Compound: 1
    Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HepG2 IC50
    6.13 3
    Compound: 1
    Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    Huh-7 CC50
    > 50 3
    Compound: 25
    Cytotoxicity against human Huh7.5.1 cells by MTT assay
    Cytotoxicity against human Huh7.5.1 cells by MTT assay
    [PMID: 22445328]
    HEK293 IC50
    1 3
    Compound: 1
    Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HepG2 IC50
    10 3
    Compound: 4
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    [PMID: 17949858]
    Huh-7 EC50
    20.8 3
    Compound: 25
    Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
    Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
    [PMID: 22445328]
    HepG2 IC50
    10 3
    Compound: 4
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    [PMID: 17949858]
    HepG2 2.2.15 IC50
    300 6
    Compound: 54
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV DNA production
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV DNA production
    [PMID: 24549242]
    MCF7 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
    Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    HepG2 IC50
    6.13 3
    Compound: 1
    Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    Huh-7 CC50
    >50 3
    Compound: 25
    Cytotoxicity against human Huh7.5.1 cells by MTT assay
    Cytotoxicity against human Huh7.5.1 cells by MTT assay
    [PMID: 22445328]
    HepG2 2.2.15 IC50
    300 6
    Compound: 54
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV DNA production
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV DNA production
    [PMID: 24549242]
    Huh-7 EC50
    20.8 3
    Compound: 25
    Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
    Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
    [PMID: 22445328]
    MCF7 IC50
    4.14 3
    Compound: 1
    Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HepG2 2.2.15 IC50
    300 6
    Compound: 54
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV e antigen secretion
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV e antigen secretion
    [PMID: 24549242]
    MCF7 IC50
    4.14 3
    Compound: 1
    Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    MDA-MB-231 IC50
    12.24 3
    Compound: 1
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    HepG2 2.2.15 IC50
    300 6
    Compound: 54
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV surface antigen secretion
    Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV surface antigen secretion
    [PMID: 24549242]
    MCF7 IC50
    >20 3
    Compound: AE
    Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
    Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    MOLT-4 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
    Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    OVCAR-3 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
    Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    Huh-7 CC50
    > 50 3
    Compound: 25
    Cytotoxicity against human Huh7.5.1 cells by MTT assay
    Cytotoxicity against human Huh7.5.1 cells by MTT assay
    [PMID: 22445328]
    MDA-MB-231 IC50
    12.24 3
    Compound: 1
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    SK-OV-3 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
    Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    MOLT-4 IC50
    >20 3
    Compound: AE
    Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
    Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    Huh-7 EC50
    20.8 3
    Compound: 25
    Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
    Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
    [PMID: 22445328]
    OVCAR-3 IC50
    >20 3
    Compound: AE
    Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
    Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    SK-OV-3 IC50
    >20 3
    Compound: AE
    Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
    Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    MCF7 IC50
    4.14 3
    Compound: 1
    Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    MCF7 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
    Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    MDA-MB-231 IC50
    12.24 3
    Compound: 1
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 34124677]
    MOLT-4 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
    Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    OVCAR-3 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
    Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    SK-OV-3 IC50
    > 20 3
    Compound: AE
    Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
    Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
    [PMID: 24900344]
    In Vitro

    Aloe emodin (0-15 μM; 24-96 h) inhibits PC3 cell proliferation in a dose-dependent manner[1].
    Aloe emodin (0-15 μM; 24 h) inhibits the activation of mTORC2 downstream substrates, Akt and PKCα. Aloe emodin could bind with mTORC2 in cells and inhibit its kinase activity[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: PC3 cells
    Concentration: 0 μM, 2.5 μM, 5 μM, 10 μM or 15 μM
    Incubation Time: 24 h, 48 h, 72 h or 96 h
    Result: Suppressed proliferation and anchorage-independent growth of PC3 cells.

    Western Blot Analysis[1]

    Cell Line: PC3 cells
    Concentration: 0 μM, 2.5 μM, 5 μM, 10 μM or 15 μM
    Incubation Time: 24 h
    Result: Inhibited the activation of the downstream substrates of mTORC2, Akt and PKCα.
    In Vivo

    Aloe emodin (10-50 mg/kg) suppresses tumor growth by inhibiting mTOR complex2 activity[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Athymic nude mice (BALB/c nude mouse, 6 weeks old)[1]
    Dosage: 10 mg/kg, 50 mg/kg (20% PEG400 in autoclaved PBS)
    Administration: Intraperitoneal injection, five times per week; for 28 days
    Result: Exhibited tumor suppression effects in vivo in an athymic nude mouse model.
    Masse moléculaire

    270.24

    Formule

    C15H10O5

    CAS No.
    Appearance

    Solid

    Color

    Brown to orange

    SMILES

    O=C1C2=C(C=CC=C2O)C(C3=CC(CO)=CC(O)=C13)=O

    Structure Classification
    Initial Source
    Livraison

    Room temperature in continental US; may vary elsewhere.

    Stockage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvant et solubilité
    In Vitro: 

    DMSO : ≥ 20.83 mg/mL (77.08 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7004 mL 18.5021 mL 37.0041 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.08 mg/mL (7.70 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (7.70 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation

    Purity: 98.32%

    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.7004 mL 18.5021 mL 37.0041 mL 92.5104 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
    15 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
    20 mM 0.1850 mL 0.9251 mL 1.8502 mL 4.6255 mL
    25 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7004 mL
    30 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
    40 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
    50 mM 0.0740 mL 0.3700 mL 0.7401 mL 1.8502 mL
    60 mM 0.0617 mL 0.3084 mL 0.6167 mL 1.5418 mL
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Nom du produit:
    Aloe emodin
    Cat. No.:
    HY-N0189
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