2. Others Anti-infection Neuronal Signaling Membrane Transporter/Ion Channel Apoptosis
  3. nAChR Environmental Pollutants Bacterial Caspase Apoptosis
  4. Benzethonium chloride

塩化ベンゼトニウム  (Synonyms: Benzethonium (chloride))

製品番号: HY-B0942 純度: 98.47%
COA 取扱説明書 Technical Support

Benzethonium chloride inhibits nicotinic acetylcholine receptors in human recombinant α7 and α4β2 neurons in Xenopus laevis oocytes, which has antibacterial, anticancer, antisepsis and disinfection activity. Benzethonium chloride induced Apoptosis and activated caspases in cancer cell lines. Benzethonium chloride ablates the tumor-forming ability of FaDu cells, delays the growth of xenograft tumors in vivo.

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Benzethonium chloride

塩化ベンゼトニウム 構造式

CAS 番号 : 121-54-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
100 mg $30 在庫あり
500 mg $60 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Benzethonium chloride:

塩化ベンゼトニウム 関連抗体

Top Publications Citing Use of Products

Caspase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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  • 純度とドキュメンテーション

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製品説明

Benzethonium chloride inhibits nicotinic acetylcholine receptors in human recombinant α7 and α4β2 neurons in Xenopus laevis oocytes, which has antibacterial, anticancer, antisepsis and disinfection activity. Benzethonium chloride induced Apoptosis and activated caspases in cancer cell lines. Benzethonium chloride ablates the tumor-forming ability of FaDu cells, delays the growth of xenograft tumors in vivo[1][2][3].

Cellular Effect
Cell Line Type Value Description References
A549 IC50
3.2 μM
Compound: BztCl
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
[PMID: 23792315]
CCRF-CEM IC50
3.8 μM
Compound: BztCl
Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay
Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay
[PMID: 23792315]
HeLa IC50
5.7 μM
Compound: BztCl
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
[PMID: 23792315]
HUVEC IC50
47 μM
Compound: BztCl
Cytotoxicity against HUVEC after 72 hrs by MTT assay
Cytotoxicity against HUVEC after 72 hrs by MTT assay
[PMID: 23792315]
MCF7 IC50
5 μM
Compound: BztCl
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
[PMID: 23792315]
MDA-MB-231 IC50
4.8 μM
Compound: BztCl
Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay
[PMID: 23792315]
Vero EC50
0.01 μg/mL
Compound: hyamine
Cytotoxicity against african green monkey Vero cells
Cytotoxicity against african green monkey Vero cells
[PMID: 21546254]
体外実験

Benzethonium chloride (0.1 g/L, 10 s) results in only a 50% reduction in bacterial number against S. mutans MT8148 and Streptococcus sobrinus[1].
Benzethonium chloride (0.1-100 μM, 48 h) reduces cell viability against FaDu and C666-1 (both human cancer) cell lines with EC50 values of 3.8 and 5.3 μM, respectively[2].
Benzethonium chloride (9 μM, 24-48 h) induces apoptosis and caspase activation in FaDu cells[2].
Benzethonium chloride (0.1 and 5 mg/L, 24-72 h) shows great acute toxicity to C. elegans, induces an inhibition on hatching and increases mortality in zebrafish embryos[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

塩化ベンゼトニウム 関連抗体

Apoptosis Analysis[2]

Cell Line: FaDu cells
Concentration: 9 μM
Incubation Time: 24-48 h
Result: Revealed nuclear condensation and blebbing and Caspase-2, caspase-8, and caspase-9 activations, indicative of apoptosis, after 48 hours of treatment.
体内実験

Benzethonium chloride (5 mg/kg, i.p., daily for 5 days) results in elimination of tumor formation and growth delay in established xenograft tumors[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Established xenograft tumors of mice[2]
Dosage: 5 mg/kg
Administration: i.p., daily for 5 days
Result: Effectively eliminates the tumorforming potential of FaDu cells and survived for 3.5 days more than PBS-treated mice, which was a statistically significant delay.
分子量

448.08

分子式

C27H42ClNO2
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

CC(C)(C)CC(C1=CC=C(OCCOCC[N+](C)(C)CC2=CC=CC=C2)C=C1)(C)C.[Cl-]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

DMSO : 33.33 mg/mL (74.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 20 mg/mL (44.63 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2317 mL 11.1587 mL 22.3174 mL
5 mM 0.4463 mL 2.2317 mL 4.4635 mL
10 mM 0.2232 mL 1.1159 mL 2.2317 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 120 mg/mL (267.81 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.2317 mL 11.1587 mL 22.3174 mL 55.7936 mL
5 mM 0.4463 mL 2.2317 mL 4.4635 mL 11.1587 mL
10 mM 0.2232 mL 1.1159 mL 2.2317 mL 5.5794 mL
15 mM 0.1488 mL 0.7439 mL 1.4878 mL 3.7196 mL
20 mM 0.1116 mL 0.5579 mL 1.1159 mL 2.7897 mL
25 mM 0.0893 mL 0.4463 mL 0.8927 mL 2.2317 mL
30 mM 0.0744 mL 0.3720 mL 0.7439 mL 1.8598 mL
40 mM 0.0558 mL 0.2790 mL 0.5579 mL 1.3948 mL
DMSO 50 mM 0.0446 mL 0.2232 mL 0.4463 mL 1.1159 mL
60 mM 0.0372 mL 0.1860 mL 0.3720 mL 0.9299 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Benzethonium chloride Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benzethonium121-54-0nAChREnvironmental PollutantsBacterialCaspaseApoptosisNicotinic acetylcholine receptorsEnvironmental Contaminantsnicotinic acetylcholine receptorsα7α4β2Xenopus laevis oocytesantibacterialanticancerantisepsisdisinfectioncaspasestumorInhibitorinhibitorinhibit

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