2. Others Neuronal Signaling Membrane Transporter/Ion Channel Metabolic Enzyme/Protease
  3. nAChR Environmental Pollutants Endogenous Metabolite
  4. Choline chloride

塩化コリン  (Synonyms: Choline (chloride))

製品番号: HY-B1337 純度: 99.94%
COA 取扱説明書 Technical Support

Choline chloride is an essential nutrient that activates alpha7 nicotinic receptors and has analgesic and anti-inflammatory activity. Glycerophosphoinositol choline can affect diseases such as liver disease, atherosclerosis and neurological disorders.

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CAS 番号 : 67-48-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in DMSO USD 66 在庫あり
Solid
100 mg $60 在庫あり
500 mg $70 在庫あり
1 g $80 在庫あり
5 g $120 在庫あり
10 g $150 在庫あり
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of Choline chloride:

塩化コリン 関連抗体

Top Publications Citing Use of Products

    Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573.  [Abstract]

    Choline (30 μM, 24 h) demonstrated no cytotoxic effects but partially mitigated the inhibition of cell viability induced by EHDPP in HTR-8/SVneo and JEG-3 cells.

    Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573.  [Abstract]

    Choline (30 μM, 24 h) reversed EHDPP-induced cell migration inhibition in HTR-8/SVneo cells.

    Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573.  [Abstract]

    Choline (30 μM, 24 h) reversed the EHDPP-induced decrease of mRNA levels of TNF-α, IL-6, and IL-1β in HTR-8/SVneo cells.

    Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573.  [Abstract]

    Choline supplementation (25 mM choline-containing water for 31.5 days) significantly increased uterine size, fetal weight, placental weight, and placental diameter that were reduced by EHDPP exposure.

    Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573.  [Abstract]

    Choline supplementation (25 mM choline-containing water for 31.5 days) alleviated the decrease in mRNA expression of the proliferation marker Ki67 and the vascular differentiation marker CD31 induced by EHDPP.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Choline chloride is an essential nutrient that activates alpha7 nicotinic receptors and has analgesic and anti-inflammatory activity. Glycerophosphoinositol choline can affect diseases such as liver disease, atherosclerosis and neurological disorders[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    17 μM
    Compound: 6209
    Substrate uptake and inhibition of the High Affinity Choline Transporter 1 (CHT1, SLC5A7) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC5A7 cells
    Substrate uptake and inhibition of the High Affinity Choline Transporter 1 (CHT1, SLC5A7) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC5A7 cells
    		10.5281/zenodo.7361355
    HEK293 EC50
    17 μM
    Compound: 6209
    Substrate uptake and inhibition of the High Affinity Choline Transporter 1 (CHT1, SLC5A7) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC5A7 cells (PubChem AID: 1794811)
    Substrate uptake and inhibition of the High Affinity Choline Transporter 1 (CHT1, SLC5A7) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC5A7 cells (PubChem AID: 1794811)
    		10.5281/zenodo.7361355
    体外実験

    Choline chloride (0 or 70 μM, 4 days) can effectively mitigate apoptosis and maintain cell viability[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    塩化コリン 関連抗体

    Cell Viability Assay[1]

    Cell Line: Rat pheochromocytoma cells PC12
    Concentration: 0 or 70 μM
    Incubation Time: 4 days
    Result: Showed the cell viability of 94% at 70 μM while cell viability of 83% at 0 μM.
    Reduced the number of cells with DNA breaks (characteristic of apoptosis) by 8.5% at 70 μM compared to the no treatment group.
    体内実験

    Choline chloride (s.c., 0.2 and 100 mg/kg/h, 24 or 48 hours) can reduce postoperative injurious reflexes and effectively decreases tumor necrosis factor (TNF) release from macrophages in female C57/Bl6 mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Postoperative pain model of female C57/Bl6 mice[2]
    Dosage: 0.2 and 100 mg/kg/h
    Administration: Subcutaneous injection, 24 or 48 hours
    Result: Reduced heat hypersensitivity after surgery with maximal efficacy after 48 h treatment and the ED50 value of choline dose was 1.7 mg/kg/h.
    Reduced hypersensitivity to punctate mechanical stimuli 48 hours after infusion in a dose-dependent manner and with the ED50 value of 4.7 mg/kg/h but not 24 hours.
    臨床実験
    分子量

    139.62

    分子式

    C5H14ClNO
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OCC[N+](C)(C)C.[Cl-]
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    Store at room temperature, keep dry and cool

    *In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature, keep dry and cool)
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 140 mg/mL (1002.72 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 100 mg/mL (716.23 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.1623 mL 35.8115 mL 71.6230 mL
    5 mM 1.4325 mL 7.1623 mL 14.3246 mL
    10 mM 0.7162 mL 3.5811 mL 7.1623 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 130 mg/mL (931.10 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.94%

    COA (245 KB) HNMR (116 KB) RP-HPLC (242 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 7.1623 mL 35.8115 mL 71.6230 mL 179.0574 mL
    5 mM 1.4325 mL 7.1623 mL 14.3246 mL 35.8115 mL
    10 mM 0.7162 mL 3.5811 mL 7.1623 mL 17.9057 mL
    15 mM 0.4775 mL 2.3874 mL 4.7749 mL 11.9372 mL
    20 mM 0.3581 mL 1.7906 mL 3.5811 mL 8.9529 mL
    25 mM 0.2865 mL 1.4325 mL 2.8649 mL 7.1623 mL
    30 mM 0.2387 mL 1.1937 mL 2.3874 mL 5.9686 mL
    40 mM 0.1791 mL 0.8953 mL 1.7906 mL 4.4764 mL
    50 mM 0.1432 mL 0.7162 mL 1.4325 mL 3.5811 mL
    60 mM 0.1194 mL 0.5969 mL 1.1937 mL 2.9843 mL
    80 mM 0.0895 mL 0.4476 mL 0.8953 mL 2.2382 mL
    100 mM 0.0716 mL 0.3581 mL 0.7162 mL 1.7906 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Choline chloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Choline67-48-1nAChREnvironmental PollutantsEndogenous MetaboliteNicotinic acetylcholine receptorsEnvironmental Contaminantscholinealpha7 nicotinic receptoranalgesicanti-inflammatoryInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-B1337
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