2. Apoptosis Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Apoptosis Endogenous Metabolite Reactive Oxygen Species (ROS) Caspase
  4. Citicoline

Citicoline  (Synonyms: Cytidine diphosphate-choline; CDP-Choline; Cytidine 5'-diphosphocholine)

Cat. No.: HY-B0739 Purity: 99.57%
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Citicoline is an endogenous intermediate in the synthesis of phosphatidylcholine which is a component of cell membranes. Citicoline inhibits reactive oxygen species (ROS) and apoptosis. Citicoline can be used for neurological disease and hearing loss study.

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CAS No. : 987-78-0

사이즈 가격 재고 수량
100 mg 해외재고보유
500 mg 해외재고보유
1 g   견적 받기  
5 g   견적 받기  

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 4 publication(s) in Google Scholar

Other Forms of Citicoline:

Citicoline Related Antibodies

Top Publications Citing Use of Products

    Citicoline purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 Apr;26(4):542-554.  [Abstract]

    In vivo EEG and ex vivo MEA recordings of KA epileptic mice treated with CDP-choline (Citicoline: 100 mg/kg) or vehicle control.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    View All Caspase Isoform Specific Products:

    View All Isoforms
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • Biological Activity

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    Citicoline is an endogenous intermediate in the synthesis of phosphatidylcholine which is a component of cell membranes. Citicoline inhibits reactive oxygen species (ROS) and apoptosis. Citicoline can be used for neurological disease and hearing loss study[1][2][3].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Caspase-8

     

    Caspase-9

     

    Caspase 3

     

    In Vitro

    Citicoline (1-100 μM, 24 h or 24 h before glutamate treatment) increases rats retinal cells viability after glutamate-induced excitotoxicity, and has synergistic effects with Tramiprosate (Homotaurine) (HY-14602)[1].
    Citicoline (100 μM, 24 h before glutamate treatment and 30 min before HG treatment) decreases proapoptotic effects associated with neuroretinal degeneration such as glutamate-induced excitotoxicity and high glucose (HG)-induced neurotoxicity, and has synergistic effects with Tramiprosate in rats retinal cells[1].
    Citicoline (100 μM, 36-48 h) reduces apoptosis in mice cochlear hair cells (HCs) and HEI-OC-1 cells after Neomycin (HY-150520) exposure, decreases the mRNA level of pro-apoptotic factor and increases anti-apoptotic factor, suppresses proapoptotic protein expression[2][3].
    Citicoline (100 μM, 36-48 h) attenuates oxidative stress in mice cochlear HCs and HEI-OC-1 cells after Neomycin injury[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Citicoline Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Rat retinal cells
    Concentration: 1, 10, 100 μM
    Incubation Time: 24 h or 24 h before glutamate treatment (100 μM)
    Result: Did not affect viability of primary retinal cultures.
    Significantly increased cell viability after glutamate-induced excitotoxicity, and had synergistic effects with homotaurine

    Apoptosis Analysis[1]

    Cell Line: Rat retinal cells
    Concentration: 100 μM
    Incubation Time: 24 h before glutamate treatment and 30 min before HG treatment
    Result: Reduced apoptosis induced by administration of glutamate and HG, and had synergistic effects with homotaurine.

    Apoptosis Analysis[3]

    Cell Line: HEI-OC-1, mice cochlear tissue
    Concentration: 10 μM
    Incubation Time: 36 or 48 h (pretreatment for 12 h and then treated with 10 mM neomycin together for 12 and 24 h in cochlear tissue and HEI-OC-1, respectively, then allowed to recover in culture medium for another 12
    Result: Showed significantly lower numbers of cleaved caspase 3-positive cells and TUNEL-positive cells than the neomycin-only group.
    Significantly reduced the percentage of apoptosis.
    The protein levels of cleaved caspase 3 were decreased compared with the Neomycin-only group.
    Decreased the pro-apoptotic factor Bax, Casp3, Casp8, and Casp9, and increased anti-apoptotic factor Bcl-2 by qPT-PCR analysis.
    In Vivo

    Citicoline (500-1000 mg/kg; i.p.; 1 h before test) produces an anticonvulsant effect in pentylenetetrazole induced convulsion in male C57Bl/6 mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57Bl/6 mice (23-27 g) (The solution of convulsant pentylenetetrazole (1%) was infused intravenously at a flow rate of 0.01 ml/sec.)[2]
    Dosage: 500, 1000 mg/kg
    Administration: Intraperitoneal injection (i.p.); 1 h before test
    Result: Increased the threshold of clonic seizures and tonic phase of seizures with lethal outcome in a dose of 500 mg/kg.
    The anticonvulsant effect was most pronounced in a dose of 1000 mg/kg.
    분자량

    488.32

    화학식

    C14H26N4O11P2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(C=CN1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OCC[N+](C)(C)C)([O-])=O)(O)=O)O2)=NC1=O
    Structure Classification
    Initial Source

    Widespread
    선적

    Room temperature in continental US; may vary elsewhere.
    보관
    Powder -20°C 3 years
    4°C 2 years

    *The compound is unstable in solutions, freshly prepared is recommended.

    용액&용해도
    In Vitro: 

    H2O : 50 mg/mL (102.39 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0478 mL 10.2392 mL 20.4784 mL
    5 mM 0.4096 mL 2.0478 mL 4.0957 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • 몰농도 계산기

    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (102.39 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    순도&문서
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.0478 mL 10.2392 mL 20.4784 mL 51.1959 mL
    5 mM 0.4096 mL 2.0478 mL 4.0957 mL 10.2392 mL
    10 mM 0.2048 mL 1.0239 mL 2.0478 mL 5.1196 mL
    15 mM 0.1365 mL 0.6826 mL 1.3652 mL 3.4131 mL
    20 mM 0.1024 mL 0.5120 mL 1.0239 mL 2.5598 mL
    25 mM 0.0819 mL 0.4096 mL 0.8191 mL 2.0478 mL
    30 mM 0.0683 mL 0.3413 mL 0.6826 mL 1.7065 mL
    40 mM 0.0512 mL 0.2560 mL 0.5120 mL 1.2799 mL
    50 mM 0.0410 mL 0.2048 mL 0.4096 mL 1.0239 mL
    60 mM 0.0341 mL 0.1707 mL 0.3413 mL 0.8533 mL
    80 mM 0.0256 mL 0.1280 mL 0.2560 mL 0.6399 mL
    100 mM 0.0205 mL 0.1024 mL 0.2048 mL 0.5120 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Citicoline Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Citicoline987-78-0Cytidine diphosphate-choline CDP-Choline Cytidine 5'-diphosphocholineApoptosisEndogenous MetaboliteReactive Oxygen Species (ROS)Caspasereactive oxygen speciesneurologicalhearing lossInhibitorinhibitorinhibit

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    상품명:
    Citicoline
    Cat. No.:
    HY-B0739
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