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  4. Citropten

Citropten  (Synonyms: Citropten; Limettin; 5,7-Dimethoxycoumarin)

製品番号: HY-N7085 純度: 99.28%
COA 取扱説明書 Technical Support

Citropten (5, 7-dimethoxycoumarin) is one of the coumarin derivatives. Citropten is an effective oral anticancer agent. Citropten has anti-proliferative activity against A2058 and B16 melanoma cells. Citropten exerts anti-inflammatory effects through the NFκB and MAPK signaling pathways. Citropten acts as an antidepressant through heat shock protein-70, monoamine oxidase-A and inhibition of apoptosis .

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Citropten

Citropten 構造式

CAS 番号 : 487-06-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
50 mg $30 在庫あり
100 mg $50 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Citropten 関連抗体

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p38 MAPK アイソフォーム固有の製品をすべて表示:

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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JNK1 JNK2 JNK3 JNK

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ERK ERK1 ERK2 ERK5 ERK8

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製品説明

Citropten (5, 7-dimethoxycoumarin) is one of the coumarin derivatives. Citropten is an effective oral anticancer agent. Citropten has anti-proliferative activity against A2058 and B16 melanoma cells. Citropten exerts anti-inflammatory effects through the NFκB and MAPK signaling pathways. Citropten acts as an antidepressant through heat shock protein-70, monoamine oxidase-A and inhibition of apoptosis[1] [2] [3].

体外実験

Citropten (10-500 mM; 72 h) inhibits the proliferation in A2058 human melanoma cell line[1].
Citropten (0-40 μM; 24 h) reduces NFκB and MAPK signaling pathway in activated Jurkat and HT-29 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Citropten 関連抗体

Cell Proliferation Assay[1]

Cell Line: A2058 human melanoma cell
Concentration: 10, 50, 100, 150 and 500 mM
Incubation Time: 72 h
Result: Inhibited cell proliferation in a concentration‐dependent manner.

Cell Proliferation Assay[2]

Cell Line: Activated Jurkat and HT-29 cells
Concentration: 0, 10, 20 and 40 μM
Incubation Time: 24 h
Result: Inhibited the phosphorylation levels of IκBα, ERK, p38 and JNK.
体内実験

Citropten (10 mg/kg; Oral administration; Every day; 7 days) has anti-inflammatory effects and modulates T cell and intestinal epithelial cell activity in mouse models of colitis induced by DSS (HY-116282)[2].
Citropten (10 mg/kg; Intraperitoneal injection; Single injection) exerts an antidepressant effect by increasing the level of heat shock protein-70 and inhibiting the level of monoamine oxidase-A in rat models of chronic mild stress depression[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS (HY-116282) treated C57BL/6J female mice aged 6-8 weeks old[2]
Dosage: 10 mg/kg
Administration: Oral administration (p.o.); every day; 7 days
Result: Ameliorated the severity of inflammation on colonic tissues.
Reduced cytokine (TNFα, IL-1β and IL-8) production on colonic tissues.
Attenuated the activity of effector T cells.
Animal Model: Chronic mild stress treated male adult Sprague-Dawley rats (around 190 g)[3]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection (i.p.), Single injection
Result: Prevented the chronic mild stress induced damage to locomotor activity.
Inhibited chronic mild stress induced increase in level of monoamine oxidase-A.
Inhibited the chronic mild stress induced increase in level ofcaspase-3.
Increased the level of heat shock protein-70 in the rat hippocampus.
分子量

206.20

分子式

C11H10O4
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

O=C1OC2=C(C(OC)=CC(OC)=C2)C=C1
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (121.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8497 mL 24.2483 mL 48.4966 mL
5 mM 0.9699 mL 4.8497 mL 9.6993 mL
10 mM 0.4850 mL 2.4248 mL 4.8497 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (10.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (10.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.41%

COA (249 KB) HNMR (237 KB) LCMS (267 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.8497 mL 24.2483 mL 48.4966 mL 121.2415 mL
5 mM 0.9699 mL 4.8497 mL 9.6993 mL 24.2483 mL
10 mM 0.4850 mL 2.4248 mL 4.8497 mL 12.1242 mL
15 mM 0.3233 mL 1.6166 mL 3.2331 mL 8.0828 mL
20 mM 0.2425 mL 1.2124 mL 2.4248 mL 6.0621 mL
25 mM 0.1940 mL 0.9699 mL 1.9399 mL 4.8497 mL
30 mM 0.1617 mL 0.8083 mL 1.6166 mL 4.0414 mL
40 mM 0.1212 mL 0.6062 mL 1.2124 mL 3.0310 mL
50 mM 0.0970 mL 0.4850 mL 0.9699 mL 2.4248 mL
60 mM 0.0808 mL 0.4041 mL 0.8083 mL 2.0207 mL
80 mM 0.0606 mL 0.3031 mL 0.6062 mL 1.5155 mL
100 mM 0.0485 mL 0.2425 mL 0.4850 mL 1.2124 mL
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Citropten Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Citropten487-06-95,7-Dimethoxycoumarin Limettinp38 MAPKEnvironmental PollutantsJNKERKEnvironmental Contaminantsc-Jun N-terminal kinaseExtracellular signal regulated kinasesA2058 and B16 melanoma cellsColitis modelDepressive disorderJurkat cellHT-29cellInhibitorinhibitorinhibit

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