2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease NF-κB Neuronal Signaling Membrane Transporter/Ion Channel Immunology/Inflammation Stem Cell/Wnt MAPK/ERK Pathway
  3. PPAR NF-κB Calcium Channel Reactive Oxygen Species (ROS) ERK
  4. Ophiopogonin D

Ophiopogonin D can be isolated from the tubers of Ophiopogon japonicus, is a rare naturally occurring C29 steroidal glycoside. Ophiopogonin D is a CYP2J3 inducer that significantly inhibits Ang II induced NF-κB nuclear translocation, IκBα down-regulation, intracellular Ca2+ overload and activation of pro-inflammatory cytokines by increasing the expression of CYP2J2/EETs and PPARα in human umbilical vein endothelial cells (HUVECs). Ophiopogonin D can inhibit isteoclastic differentiation in RAW264.7 cells. Ophiopogonin D has protective effect as an antioxidant in H2O2-induced endothelial injury. Ophiopogonin D blocks ERK signaling cascades. Ophiopogonin D alleviates high-fat diet-induced metabolic syndrome and changes the structure of gut microbiota in mice. Ophiopogonin D has been used against inflammatory, metabolic and cardiovascular diseases.

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Ophiopogonin D

Ophiopogonin D 화학구조

CAS No. : 945619-74-9

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of Ophiopogonin D:

Ophiopogonin D Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ophiopogonin D

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PPARα PPARβ/δ PPARγ PPAR PPARδ

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Calcium Channel Isoform Specific Products:

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

View All ERK Isoform Specific Products:

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Ophiopogonin D can be isolated from the tubers of Ophiopogon japonicus, is a rare naturally occurring C29 steroidal glycoside. Ophiopogonin D is a CYP2J3 inducer that significantly inhibits Ang II induced NF-κB nuclear translocation, IκBα down-regulation, intracellular Ca2+ overload and activation of pro-inflammatory cytokines by increasing the expression of CYP2J2/EETs and PPARα in human umbilical vein endothelial cells (HUVECs). Ophiopogonin D can inhibit isteoclastic differentiation in RAW264.7 cells. Ophiopogonin D has protective effect as an antioxidant in H2O2-induced endothelial injury. Ophiopogonin D blocks ERK signaling cascades. Ophiopogonin D alleviates high-fat diet-induced metabolic syndrome and changes the structure of gut microbiota in mice. Ophiopogonin D has been used against inflammatory, metabolic and cardiovascular diseases[1][2][3][4][5].

IC50 & Target

PPARα

 

NF-κB

 

In Vitro

Ophiopogonin D (Compound OP-D) (0.1-200 μM, 24-48 h) only provokes cell viability at high concentrations above 40 μM in HUVECs[2].
Ophiopogonin D (5-20 μM, 24 h) significantly increases the expression of CYP2J2 and PPARα protein and mRNA expression dose-dependently[2].
Ophiopogonin D (1-100 μM, 24 h prior to H2O2) inhibits H2O2-induced cytotoxicity in MC3T3-E1 and RAW264.7 cells[3].
Ophiopogonin D (1-100 μM) inhibits osteoclastic differentiation in RAW264.7 cells[3].
Ophiopogonin D (0.6-60 μM, 2 h) dose-dependently prevents H2O2-induced oxidative stress in HUVECs[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ophiopogonin D Related Antibodies

ELISA Assay[2]

Cell Line: HUVECs
Concentration: 5, 10, 20 μM
Incubation Time: 24 h
Result: Significantly increased 11,12-DHET levels.

Western Blot Analysis[2]

Cell Line: HUVECs
Concentration: 5, 10, 20 μM
Incubation Time: 24 h
Result: Suppressed Ang II-induced inflammatory responses via CYP2J2-PPARα pathway.
In Vivo

Ophiopogonin D (Compound OP-D) (5-25 mg/kg, i.p., daily for 12 w) partially ameliorates the increased SMI and Tb.Sp that attributes to the ovariectomy in OVX mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c female OVX mice (19-21 g)[3]
Dosage: 5, 25 mg/kg
Administration: Intraperitoneal injection (i.p.), daily for 12 w
Result: Inhibited serum osteoclastic markers in serum, such as CTX-1 and TRAP.
Partially inhibited osteoclastogenesis.
분자량

855.02

화학식

C44H70O16
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6([C@@H](C[C@H](O)C5)O[C@@](O[C@H](C)[C@H](O)[C@@H]7O[C@@](OC[C@@H](O)[C@@H]8O)([H])[C@@H]8O)([H])[C@@H]7O[C@@](O[C@@H](C)[C@H](O)[C@H]9O)([H])[C@@H]9O)C)([H])[C@]6([H])CC2
Structure Classification
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도
In Vitro: 

DMSO : 100 mg/mL (116.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1696 mL 5.8478 mL 11.6956 mL
5 mM 0.2339 mL 1.1696 mL 2.3391 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서

Purity: 99.80%

SDS (393 KB)

COA (257 KB) HNMR (322 KB) RP-HPLC (210 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1696 mL 5.8478 mL 11.6956 mL 29.2391 mL
5 mM 0.2339 mL 1.1696 mL 2.3391 mL 5.8478 mL
10 mM 0.1170 mL 0.5848 mL 1.1696 mL 2.9239 mL
15 mM 0.0780 mL 0.3899 mL 0.7797 mL 1.9493 mL
20 mM 0.0585 mL 0.2924 mL 0.5848 mL 1.4620 mL
25 mM 0.0468 mL 0.2339 mL 0.4678 mL 1.1696 mL
30 mM 0.0390 mL 0.1949 mL 0.3899 mL 0.9746 mL
40 mM 0.0292 mL 0.1462 mL 0.2924 mL 0.7310 mL
50 mM 0.0234 mL 0.1170 mL 0.2339 mL 0.5848 mL
60 mM 0.0195 mL 0.0975 mL 0.1949 mL 0.4873 mL
80 mM 0.0146 mL 0.0731 mL 0.1462 mL 0.3655 mL
100 mM 0.0117 mL 0.0585 mL 0.1170 mL 0.2924 mL
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Ophiopogonin D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ophiopogonin D945619-74-9PPARNF-κBCalcium ChannelReactive Oxygen Species (ROS)ERKPeroxisome proliferator-activated receptorsNuclear factor-κBNuclear factor-kappaBCa2+ channelsCa channelsExtracellular signal regulated kinasesInhibitorinhibitorinhibit

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상품명:
Ophiopogonin D
Cat. No.:
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