1. Recombinant Proteins
  2. Organoid Culture

An organoid is a 3D multicellular in vitro tissue construct that mimics the complex structure and functionality of corresponding in vivo organ. Organoids can be generated from stem cells in adult tissue or from pluripotent stem cells. Organoid structures provide a very useful tool as a model to study tissue development and disease, personalized medicine, drug screening and cell therapy
Organoids recapitulate multiple biological parameters including the spatial organization of heterogeneous tissue-specific cells, cell-cell interactions, and cell-matrix interactions, and certain physiological functions generated by tissue-specific cells within the organoid. They are more physiologically relevant than monolayer culture models and are far more amenable to manipulation of niche components, signalling pathways and genome editing than in vivo models.
Organoids can be fast expanded long-term, cryopreserved and applied to high-throughput analyses.

Organoids are generated from tissue-specific adult stem cells or ESCs/iPSCs. Organoids are typically cultured in an extracellular matrix (ECM) surrounded by culture media supplemented with niche factors specific to the organoid type. Common niche and ECM factors: R-spondin (RSPO1), EGF, Noggin, Activin A, Collagen.

Common Reagents for Organoid Culture

Recombinant Proteins

Cat. No Product Name Proteins Function



Human Wnt3a Surrogate

Human Wnt3a

Wnt An essential niche component for maintaining the proliferation of Lgr5-positive stem cells in various organoids, such as the intestinal, gastric, pancreatic and liver organoids.



Human EGF

Mouse EGF

EGF A growth factor for epithelial tissues; binding to EGF receptors, induces hyperplasic changes. Used for the generation of intestinal, liver, thyroid, and brain organoids.



Human Noggin

Mouse Noggin

Noggin An inhibitor of bone morphogenetic proteins that modulates cellular differentiation, proliferation, and apoptosis.



Human R-spondin-1

Mouse R-spondin-1

R-spondin The ligand of Lgr5 and a niche factor that is required for the self-renewal of stem cells and activates Wnt signaling. An essential additive of the organoid culture system.







Human FGF-4

Human FGF-7

Human FGF-9

Human FGF-10

Human FGF-19

Human FGF-basic/FGF-2

FGF FGFs play crucial roles in a wide variety of cellular functions, including cell proliferation, survival, metabolism, morphogenesis, and differentiation, as well as in tissue repair and regeneration. In a 3D extracellular matrix, FGF-2, FGF-7, FGF-9, and FGF-10 promote lung organoid formation.




Human BMP-4

Human BMP-7

Human/Mouse/Rat BMP-2

BMP BMPs play crucial roles in embryogenesis and development, and also in maintenance of adult tissue homeostasis. BMP-2 and BMP-4 are widely used in in vitro generation of hepatic cells from iPSCs and ESCs.



Human VEGF-A

Mouse VEGF-A

VEGF VEGF-A is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. It is used in the generation of vascular organoids.



PDGF PDGF-BB induces vascular smooth muscle cells (VSMC) specification and cell differentiation in the vascular.


Human HGF

HGF A known hepatocyte mitogen that can be used for the liver organoid culture.


Human/Mouse/Rat Activin A

Activin A A cytokine with multiple roles in development and homeostasis.In the case of intestinal organoids, it activates TGF-β signaling in PSCs to trigger endodermal differentiation.


Human DKK-1

DKK A canonical WNT inhibitor that can induce retinal progenitors for self-organization.

Basement Membrane Matrix

Cat. No Product Name Application


Basement Membrane Matrix (Phenol Red)

In vitro angiogenesis, tumor cell migration or invasion


Basement Membrane Matrix

In vitro angiogenesis, tumor cell migration or invasion


Basement Membrane Matrix GFR (Phenol Red)

Organoid culture, in vitro angiogenesis


Basement Membrane Matrix GFR

Organoid culture, in vitro angiogenesis


Basement Membrane Matrix HC (Phenol Red)

Transplantation/induction of tumorigenic models such as PDX, CDX


Basement Membrane Matrix IPSC-qualified

Stem cell expansion and differentiation


Basement Membrane Matrix for Organoid Culture

Organoid culture

Organoid culture kit

organoid medium Cat. No Types of tissue Cat. No Types of tissue
Tumor organoid medium HY-K6101 Human breast cancer HY-K6106 Human Cholangiocarcinoma
HY-K6102 Human Lung Adenocarcinoma HY-K6107 Human Cervical Cancer
HY-K6103 Human Small Cell Lung Cancer HY-K6108 Human Esophageal Cancer
HY-K6104 Human Colorectal Cancer HY-K6109 Human Endometrial Cancer
HY-K6105 Human Gastric Cancer HY-K6110 Human Pancreatic Cancer
HY-K6111 Human Head and Neck Squamous Cell Carcinoma HY-K6120 Mouse Colonic
Normal Tissue organoid Medium HY-K6112 Human Colonic HY-K6116 Human Kidney Tubular
HY-K6113 Human Intestinal HY-K6117 Human Liver Ductal
HY-K6114 Human Gastric Epithelial HY-K6118 Mouse Liver Ductal
HY-K6115 Human Pancreatic HY-K6119 Mouse Intestinal

Experiment validation

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5.2 ng/mL. The ED50 of human Wnt Surrogate from MedChemExpress's each Lot is lower than of Competitor P. The ED50 is <0.2 ng/mL as measured by murine BALB/c 3T3 cells. Measured by its ability to inhbit BMP-4-induced alkalnephosphatase production by ATDC5 mouse chondrogenic cells in the presence of Recombinant Human BMP-4 (40 ng/mL), with an ED50 of 4 ng/mL.


Mouse liver ductal organoids
cultured by MedChemExpress (MCE) Basement
Membrane Matrix


Mouse trachea organoids
cultured by MedChemExpress (MCE) Basement
Membrane Matrix GFR
(Phenol Red)


Mouse intestinal organoid
cultured by MedChemExpress (MCE) Basement
Membrane Matrix


Tumor volume in tumor cell-loaded tumor
model (CDX) mice by MedChemExpress (MCE) Basement
Membrane Matrix HC (Phenol Red)