Wnt3a Protein, Human (His)

Wnt3a protein is a ligand of Frizzled receptors and can activate TCF/LEF transcription factors in the canonical Wnt pathway. It is essential for embryonic mesoderm development, caudal somite formation, neural tube morphogenesis, and intestinal crypt stem cell self-renewal. Wnt3a Protein, Human (His) is the recombinant human-derived Wnt3a protein, expressed by E. coli , with N-6*His labeled tag. The total length of Wnt3a Protein, Human (His) is 333 a.a., with molecular weight of ~40 kDa.

The Wnt3a protein serves as a ligand for members of the frizzled family of seven transmembrane receptors, likely functioning in the canonical Wnt signaling pathway, resulting in the activation of transcription factors of the TCF/LEF family. It is essential for normal embryonic mesoderm development, caudal somite formation, and morphogenesis of the developing neural tube. Wnt3a also plays a role in mediating the self-renewal of stem cells at the base of intestinal crypts in vitro. The protein forms a soluble 1:1 complex with AFM, preventing oligomerization and ensuring prolonged biological activity; this complex may represent the physiological form in body fluids. Wnt3a forms homooligomers through disulfide linkages, which lead to inactivation. It interacts with PORCN and components of the Wnt-Fzd-LRP5-LRP6 signaling complex, including LRP6, APCDD1, and WLS. Additionally, Wnt3a interacts with glypican GPC3 and PKD1 via its extracellular domain. These interactions underscore the complexity of Wnt3a's involvement in various developmental and signaling processes.

Human

E. coli

N-6*His

P56704 (S19-C351)

89780  [NCBI]

SYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTC

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.