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  3. Wnt3a Surrogate Protein, Human (HEK293, C-hFc)

Wnt3a Surrogate Protein, Human (HEK293, C-hFc)

Cat. No.: HY-P70453C
COA Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
20 μg $240 In-stock
50 μg $420 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products

Publications Citing Use of MCE Wnt3a Surrogate Protein, Human (HEK293, C-hFc)

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 3.2-5.2 ng/mL.

  • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells with an ED50 of 5.2 ng/mL.
  • The ED50 of human Wnt Surrogate from MCE's each Lot is lower than of Competitor P.
Species

Human

Source

HEK293

Tag

C-hFc

Accession

/

Gene ID

/

Synonyms
Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
AA Sequence

/

Molecular Weight

60-75 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Wnt3a Surrogate Protein, Human (HEK293, C-hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Wnt3a Surrogate Protein, Human (HEK293, C-hFc)
Cat. No.:
HY-P70453C
Quantity:
MCE Japan Authorized Agent: