Cat. No.: HY-N0103

Sophocarpine

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

Sophocarpine is a PTEN activator and an inhibitor of PI3K/Akt, MEK/ERK, and NF-κB signaling pathways. Sophocarpine upregulates PTEN expression and inhibits PI3K/Akt phosphorylation, arrests tumor cell cycle and induces apoptosis. Sophocarpine inhibits MEK/ERK phosphorylation and VEGF secretion, reducing tumor cell migration. Sophocarpine can also inhibit NF-κB activation and p38 and JNK phosphorylation, reduce the expression of inflammatory factors such as iNOS and COX-2, and activate the Nrf2/HO-1 pathway to reduce oxidative stress. Sophocarpine has anti-tumor, anti-inflammatory, antioxidant and anti-apoptotic effects, and can be used in the research of cancers such as glioblastoma and colorectal cancer, inflammation-related diseases, and Doxorubicin (HY-15142A)-induced cardiac damage.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 6483-15-4
Formula: C₁₅H₂₂N₂O
Molecular Weight:246.35

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Sophocarpine Chemical Structure

CAS No.: 6483-15-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

PI3K

All NF-κB Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All MEK Isoforms :

All ERK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>40 μM Compound: 6	Antiproliferative activity against human A549 cells after 3 days by SRB assay Antiproliferative activity against human A549 cells after 3 days by SRB assay	[PMID: 26865176]
KB	IC₅₀	>40 μM Compound: 6	Antiproliferative activity against human KB cells after 3 days by SRB assay Antiproliferative activity against human KB cells after 3 days by SRB assay	[PMID: 26865176]
MDA-MB-231	IC₅₀	>40 μM Compound: 6	Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay	[PMID: 26865176]
RAW264.7	IC₅₀	>200 μM Compound: 2	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production after 6 hrs by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production after 6 hrs by ELISA	[PMID: 21036613]

In Vitro

Sophocarpine (0.5-4 mM; 24-48 h) inhibits cell viability, proliferation and migration, induces cell cycle G0/G1 arrest and apoptosis, upregulates PTEN and downregulates PI3K/Akt signaling pathways in glioblastoma cells (U251, C6)^[1].
Sophocarpine (0.4 mM; 24-72 h) inhibits cell proliferation and migration, downregulates MEK/ERK phosphorylation and VEGF-A/C/D secretion, and reduces N-cadherin and MMP-9 expression in colorectal cancer cells (HCT116, SW620)^[2].
Sophocarpine (50-100 μg/mL; 24 h) inhibits LPS-induced NO, TNF-α, and IL-6 production in RAW 264.7 macrophages, downregulates iNOS and COX-2 protein expression, and inhibits NF-κB activation and p38 and JNK phosphorylation in RAW 264.7 macrophages^[3].
Sophocarpine (1-5 μM; 24 h pretreatment + 12 h DOX cotreatment) reduces ROS levels in H9C2 cardiomyocytes, upregulates Nrf2/HO-1 signaling, inhibits DOX (HY-15142A)-induced Bax and cleaved-caspase-3 expression, and upregulates Bcl-2 in H9C2 cardiomyocytes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

U251, C6 glioblastoma cells
Concentration:

0.5, 1, 2, 4 mM
Incubation Time:

24-48 h
Result:

Reduced cell viability in a dose- and time-dependent manner, with IC₅₀ around 2 mM at 48 hours, as measured by CCK-8 assay.
No significant cytotoxicity was observed in primary astrocytes at these concentrations.

Apoptosis Analysis^[1]

Cell Line:

U251 glioblastoma cells
Concentration:

2 mM
Incubation Time:

24 h
Result:

Resulted a significant increase in subG1 phase cells (apoptotic cells) and G0/G1 phase arrest.

Western Blot Analysis^[1]^[2]

Cell Line:

U251 glioblastoma cells^[1] HCT116, SW620 colorectal cancer cells^[2]
Concentration:

2 mM for U251 cells; 0.4 mM for HCT116, SW620 cells
Incubation Time:

24 h, 48 h
Result:

Upregulation of cleaved Caspase-3, P53, and downregulation of Bcl-2, indicating induction of intrinsic apoptosis in U251 cells^[1].
Downregulated phosphorylated MEK (p-MEK), phosphorylated ERK (p-ERK), N-cadherin, and MMP-9 in HCT116, SW620 cells, while VEGF-A/C/D secretion reduced^[2].

In Vivo

Sophocarpine (35 mg/kg; intraperitoneal injection; once every 2 days; 2 weeks) inhibits tumor growth, upregulates PTEN expression, downregulates Akt phosphorylation, and induces tumor cell apoptosis in BALB/c nude mouse subcutaneous and orthotopic glioma transplantation models^[1].
Sophocarpine (10-30 mg/kg; intraperitoneal injection; twice a week; 4 weeks) improves cardiac function, reduced CK, CK-MB, and LDH levels, and alleviates oxidative stress and cardiomyocyte apoptosis in a DOX (HY-15142A)-induced cardiac injury model in C57BL/6J mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c Nude Mouse Glioblastoma Xenograft Model (male, 18-24 g, 6-8 weeks old): subcutaneous and orthotopic U251 glioblastoma xenografts^[1]
Dosage:

35 mg/kg
Administration:

Intraperitoneal injection, every 2 days for 2 weeks (subcutaneous model); daily for 2 weeks (orthotopic model)
Result:

Significantly reduced subcutaneous tumor volume and weight by 50% compared to control.
Echocardiography and histology showed no cardiotoxicity.
Western blot in tumor tissues revealed upregulated PTEN and cleaved Caspase-3, downregulated p-Akt and Bcl-2, indicating activation of the PTEN/PI3K/Akt pathway to induce apoptosis.
Immunohistochemistry confirmed reduced Ki-67 proliferation marker and increased PTEN expression.

Animal Model:

C57BL/6J Mouse Doxorubicin-Induced Heart Injury Model (male, 25 g, 7 weeks old): Doxorubicin (5 mg/kg weekly×4 weeks) induced cardiotoxicity^[4]
Dosage:

10 mg/kg or 30 mg/kg
Administration:

Intraperitoneal injection, co-administered with Doxorubicin weekly for the last 2 weeks (after initial 2 weeks of doxorubicin alone), total 4 weeks
Result:

Echocardiography showed improved left ventricular ejection fraction (LVEF) and fractional shortening (LVFS).
Serum biomarkers CK, CK-MB, and LDH were reduced by 30-40% compared to doxorubicin group.
DHE staining revealed decreased myocardial superoxide production, while MDA levels were lowered and SOD/GSH increased.
Western blot showed upregulated Nrf2/HO-1 signaling, reduced NOX-4 and pro-apoptotic proteins (Bax, cleaved-caspase-3, Cyto-C), and increased anti-apoptotic Bcl-2 in heart tissues.

Chemical Information

CAS No. 6483-15-4
Appearance Solid
Molecular Weight 246.35
Formula C₁₅H₂₂N₂O
Color White to off-white
SMILES

O=C1C=CC[C@]2([H])[C@@]3([H])CCCN4[C@@]3([H])[C@](CCC4)([H])CN21
Structure Classification
- Alkaloids
- Piperidine Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (12)

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Journal Impact Factor

Acta Pharmacol Sin

Sophocarpine alleviates chronic itch in mouse atopic dermatitis by inhibiting spinal astrocyte reactivity and pro-inflammatory signaling. [Abstract]

2026 Jan 7. PMID: 41501419
Phytomedicine

Sophocarpine suppresses MAPK-mediated inflammation by restoring gut microbiota in colorectal cancer. [Abstract]

2025 May 6:143:156833. PMID: 40393246
Phytomedicine

Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]

2025 May:140:156484. PMID: 40090046
J Ethnopharmacol

Isoxanthohumol, a component of Sophora flavescens, promotes the activation of the NLRP3 inflammasome and induces idiosyncratic hepatotoxicity. [Abstract]

2022 Mar 1:285:114796. PMID: 34740771
Int Immunopharmacol

Minimolide F alleviates inflammatory diseases by specifically targeting STING and blocking IRF3 recruitment. [Abstract]

2026 May 1:176:116468. PMID: 41819671
Eur J Pharmacol

The protective effects of sophocarpine on sepsis-induced cardiomyopathy. [Abstract]

2023 Jul 5:950:175745. PMID: 37146706

Sophocarpine purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jul 5:950:175745. [Abstract]

Sophocarpine (20 mg/kg; i.p.; daily; one week) significantly reduces the level of oxidative stress in mice.

Sophocarpine purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jul 5:950:175745. [Abstract]

Sophocarpine (20 mg/kg; i.p.; daily; one week) and LPS together significantly increases the expression of Nrf2 and HO-1 in mice.
Molecules

Sophocarpine Alleviates Isoproterenol-Induced Kidney Injury by Suppressing Inflammation, Apoptosis, Oxidative Stress and Fibrosis. [Abstract]

2022 Nov 15;27(22):7868. PMID: 36431969

Sophocarpine purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Nov 15;27(22):7868. [Abstract]

Sophocarpine (SOP; 20, 40 mg/kg; i.p.; once per day over two weeks) significantly hinders the ISO(Isoproterenol)-induced increase in TLR-4 and phosphorylation of NF-κB in mice.
FASEB J

2026 May 31;40(10):e71905. PMID: 42149696
Mediators Inflamm

Targeting Pulmonary Hypertension: Elucidating Sophocarpine's Protective Role via Preclinical Models. [Abstract]

2026 Feb 23:2026:5524066. PMID: 41737847
Sci Rep

Sophocarpine alleviates doxorubicin-induced heart injury by suppressing oxidative stress and apoptosis. [Abstract]

2024 Jan 3;14(1):428. PMID: 38172265
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
PeerJ

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer. [Abstract]

2022 Sep 16;10:e14042. PMID: 36132221

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (202.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (40.59 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0592 mL

20.2960 mL

40.5920 mL

5 mM

0.8118 mL

4.0592 mL

8.1184 mL

10 mM

0.4059 mL

2.0296 mL

4.0592 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 10 mg/mL (40.59 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xing S, et al. Sophocarpine inhibits the progression of glioblastoma via PTEN/PI3K/Akt signaling pathway. Am J Cancer Res. 2024 Aug 25;14(8):3757-3772. [Content Brief]

[2]. Wang Q, et al. Sophocarpine Inhibits Tumorgenesis of Colorectal Cancer via Downregulation of MEK/ERK/VEGF Pathway. Biol Pharm Bull. 2019 Nov 1;42(11):1830-1838. [Content Brief]

[3]. Gao Y, et al. Anti-inflammatory effects of sophocarpine in LPS-induced RAW 264.7 cells via NF-κB and MAPKs signaling pathways. Toxicol In Vitro. 2012 Feb;26(1):1-6. [Content Brief]

[4]. Zhang HJ, et al. Sophocarpine alleviates doxorubicin-induced heart injury by suppressing oxidative stress and apoptosis. Sci Rep. 2024 Jan 3;14(1):428. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	4.0592 mL	20.2960 mL	40.5920 mL	101.4800 mL
	5 mM	0.8118 mL	4.0592 mL	8.1184 mL	20.2960 mL
	10 mM	0.4059 mL	2.0296 mL	4.0592 mL	10.1480 mL
	15 mM	0.2706 mL	1.3531 mL	2.7061 mL	6.7653 mL
	20 mM	0.2030 mL	1.0148 mL	2.0296 mL	5.0740 mL
	25 mM	0.1624 mL	0.8118 mL	1.6237 mL	4.0592 mL
	30 mM	0.1353 mL	0.6765 mL	1.3531 mL	3.3827 mL
	40 mM	0.1015 mL	0.5074 mL	1.0148 mL	2.5370 mL
DMSO	50 mM	0.0812 mL	0.4059 mL	0.8118 mL	2.0296 mL
	60 mM	0.0677 mL	0.3383 mL	0.6765 mL	1.6913 mL
	80 mM	0.0507 mL	0.2537 mL	0.5074 mL	1.2685 mL
	100 mM	0.0406 mL	0.2030 mL	0.4059 mL	1.0148 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Sophocarpine

12 Cited Publications

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Sophocarpine

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