Vancomycin

Name: Vancomycin
Brand: MedChemExpress
SKU: HY-B0671
Rating: 5.0 (94 reviews)

Cat. No.: HY-B0671 Purity: 99.39%: Data Sheet
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Vancomycin is an antibiotic for the treatment of bacterial infections.

For research use only. We do not sell to patients.

CAS No. : 1404-90-6

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
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Based on 94 publication(s) in Google Scholar

Other Forms of Vancomycin:

94 Publications Citing Use of MCE Vancomycin

RT-PCR

IHC

: Vancomycin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Nov:67:102929.; ARG2 protein expressions in the kidneys of Vancomycin (500 mg/kg, daily, 7 days, ip)-induced AKI mice.

: Vancomycin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Nov:67:102929.; ARG2 mRNA expressions in the kidneys of Vancomycin (500 mg/kg, daily, 7 days, ip)-induced AKI mice.

: Vancomycin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Nov:67:102929.; Representative images of immunohistochemistry staining of ARG2 in Vancomycin (500 mg/kg, daily, 7 days, ip)-induced AKI mice kidneys. The red arrows indicate deeper ARG2-positive staining of renal tubules. Scale, 20×, 100 μm, 40×, 50 μm.

: Vancomycin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Nov:67:102929.; ARG2 protein expression in primary tubular epithelial cells treated by Vancomycin (4mM, 48 h).

: Vancomycin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Nov:67:102929.; Renal function and renal tissue injury of ARG2 KO and WT mice administrated with Vancomycin were assessed by SCr, BUN, and tubular injury score. The red arrows showed the vacuolar degeneration, tubular necrosis, cast formation, and tubular dilation of renal tubules.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Vancomycin is an antibiotic for the treatment of bacterial infections.

IC₅₀ & Target

Glycopeptide

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	IC₅₀	> 500 μM Compound: 1	Inhibitory concentration against proliferation of human T-lymphocytic (CEM) cells Inhibitory concentration against proliferation of human T-lymphocytic (CEM) cells	[PMID: 12801238]
HEK-293T	IC₅₀	> 320 μM Compound: Vancomycin	Antiproliferative activity against HEK293T cells assessed as reduction in cell viability measured for 48 hrs by MTT assay Antiproliferative activity against HEK293T cells assessed as reduction in cell viability measured for 48 hrs by MTT assay	[PMID: 38173250]
HEK293	CC₅₀	> 100 μM Compound: Vancomycin	Cytotoxicity against HEK293 cells after 72 hrs by MTT assay Cytotoxicity against HEK293 cells after 72 hrs by MTT assay	[PMID: 24751446]
HEK293	CC₅₀	> 100 μM Compound: Vancomycin	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay	[PMID: 30392371]
HEK293	CC₅₀	> 1000 μg/mL Compound: Vancomycin	Cytotoxicity against HEK293 cells after 72 hrs by MTT assay Cytotoxicity against HEK293 cells after 72 hrs by MTT assay	[PMID: 24139168]
L02	IC₅₀	> 20 μM Compound: Vancomycin	Cytotoxicity against human LO2 cells Cytotoxicity against human LO2 cells	[PMID: 33811991]
L02	IC₅₀	> 30 μM Compound: Vancomycin	Cytotoxicity against human L02 cells after 72 hrs by CCK-8 assay Cytotoxicity against human L02 cells after 72 hrs by CCK-8 assay	[PMID: 35398730]
L02	IC₅₀	> 43 μg/mL Compound: Vancomycin	Cytotoxicity against human L02 cells after 72 hrs by CCK-8 assay Cytotoxicity against human L02 cells after 72 hrs by CCK-8 assay	[PMID: 35398730]
L02	IC₅₀	> 64 μg/mL Compound: Van	Cytotoxicity against human L02 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay Cytotoxicity against human L02 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 38096652]
L1210	IC₅₀	> 1000 μg/mL Compound: vancomycin	Cytotoxicity against mouse L1210 cells assessed as cell viability by trypan blue assay Cytotoxicity against mouse L1210 cells assessed as cell viability by trypan blue assay	[PMID: 9051915]
L1210	IC₅₀	> 1000 μg/mL Compound: vancomycin	Cytotoxicity against mouse L1210 cells by MTT assay Cytotoxicity against mouse L1210 cells by MTT assay	[PMID: 9051915]
L1210	IC₅₀	> 500 μM Compound: 1	Inhibitory concentration of compound against proliferation of murine lukemia cells L1210 was determined Inhibitory concentration of compound against proliferation of murine lukemia cells L1210 was determined	[PMID: 12801238]
MOLT-4	IC₅₀	> 500 μM Compound: 1	Inhibitory concentration of compound against proliferation of human T-lymphocytic cells MOLT-4/C8 was determined Inhibitory concentration of compound against proliferation of human T-lymphocytic cells MOLT-4/C8 was determined	[PMID: 12801238]

In Vitro

Vancomycin is a large glycopeptide compound with a molecular weight of 1450 Da^[1]. Vancomycin is a unique glycopeptide structurally unrelated to any currently available antibiotic. It also has a unique mode of action inhibiting the second stage of cell wall synthesis of susceptible bacteria. Vancomycin is active against a large number of species of Gram-positive bacteria, such as Staphylococcus aureus, Staph. epidermidis, Str. agalactiae, Str. bovis, Str. mutans, viridans streptococci, enterococci^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Vancomycin can be used to induce renal injury models^[4].

Induction of Kidney Injury Model^[4]

Background

Vancomycin induces oxidative stress-related apoptosis in animals.

Specific Modeling Methods

Mice: C57BL/6 • male • 6-week-old
Administration: 400 mg/kg • ip • once daily for 7 weeks

Modeling Indicators

Molecular changes: Induced cell apoptosis and increase in kidney Cr, BUN, MDA, IL-1β, IL-6, TNF-α, and NF-κB.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J male mice^[4]
Dosage:	400 mg/kg/day, 7 day
Administration:	Intraperitoneal injection (i.p.)
Result:	Caused renal injury with a higher renal injury score and kidney index. Induced cell apoptosis and increase in kidney Cr, BUN, MDA, IL-1β, IL-6, TNF-α, and NF-κB.

Molecular Weight

1449.25

Formula

C₆₆H₇₅Cl₂N₉O₂₄

CAS No.

1404-90-6

Appearance

Solid

Color

White to light yellow

Sequence

{d-Leu}-{d-Tyr}-Asn-{d-Nty}-{d-Nty}-Tyr-{Nty} (Covalent bridge:Tyr2-Nty4, Nty4-Tyr6, Nty5-Nty7)

Sequence Shortening

{d-Leu}-{d-Tyr}-N-{d-Nty}-{d-Nty}-Y-{Nty} (Covalent bridge:Tyr2-Nty4, Nty4-Tyr6, Nty5-Nty7)

SMILES

O[C@@H]1[C@@H](O[C@@](O[C@@H](C)[C@H]2O)([H])C[C@@]2(N)C)[C@@H](O[C@H](CO)[C@H]1O)OC3=C(OC4=CC=C([C@H]([C@H]5NC([C@H](NC)CC(C)C)=O)O)C=C4Cl)C=C([C@](NC([C@@H](NC5=O)CC(N)=O)=O)([H])C(N[C@](C6=O)([H])C7=CC8=C(O)C=C7)=O)C=C3OC9=CC=C([C@H]([C@](C(N[C@@](C(O)=O)([H])C%10=C8C(O)=CC(O)=C%10)=O)([H])N6)O)C=C9Cl

Structure Classification

Initial Source

Microorganisms

Streptomyces orientalis.

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (34.50 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.6900 mL	3.4501 mL	6.9001 mL
5 mM	0.1380 mL	0.6900 mL	1.3800 mL
10 mM	0.0690 mL	0.3450 mL	0.6900 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.39%

Select Batch:

Data Sheet (293 KB) SDS (394 KB)

COA (267 KB) HNMR (423 KB) RP-HPLC (257 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Rybak MJ, et al. The pharmacokinetic and pharmacodynamic properties of vancomycin. Clin Infect Dis. 2006 Jan 1;42 Suppl 1:S35-9. [Content Brief]

[2]. Watanakunakorn C, et al. Mode of action and in-vitro activity of vancomycin. J Antimicrob Chemother. 1984 Dec;14 Suppl D:7-18. [Content Brief]

[3]. Warren CA, et al. Vancomycin treatment's association with delayed intestinal tissue injury, clostridial overgrowth, and recurrence of Clostridium difficile infection in mice. Antimicrob Agents Chemother. 2013 Feb;57(2):689-96. [Content Brief]

[4]. Juan He, et al. Vitamin C reduces vancomycin-related nephrotoxicity through the inhibition of oxidative stress, apoptosis, and inflammation in mice. Ann Transl Med. 2021 Aug; 9(16): 1319.

[5]. Ping Yu, et al. N-acetylcysteine Ameliorates Vancomycin-induced Nephrotoxicity by Inhibiting Oxidative Stress and Apoptosis in the in vivo and in vitro Models. Int J Med Sci. 2022; 19(4): 740–752.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.6900 mL	3.4501 mL	6.9001 mL	17.2503 mL
	5 mM	0.1380 mL	0.6900 mL	1.3800 mL	3.4501 mL
	10 mM	0.0690 mL	0.3450 mL	0.6900 mL	1.7250 mL
	15 mM	0.0460 mL	0.2300 mL	0.4600 mL	1.1500 mL
	20 mM	0.0345 mL	0.1725 mL	0.3450 mL	0.8625 mL
	25 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6900 mL
	30 mM	0.0230 mL	0.1150 mL	0.2300 mL	0.5750 mL

Vancomycin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vancomycin1404-90-6BacterialAutophagyAntibioticInhibitorinhibitorinhibit

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Vancomycin

Customer Review

Other Forms of Vancomycin:

Vancomycin Related Antibodies

94 Publications Citing Use of MCE Vancomycin

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Customer Review

Vancomycin Related Antibodies

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Complete Stock Solution Preparation Table

Vancomycin Related Classifications

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