1. Cell Cycle/DNA Damage
    PI3K/Akt/mTOR
    Autophagy
    Apoptosis
  2. DNA/RNA Synthesis
    Akt
    mTOR
    Autophagy
    Apoptosis
  3. 8-Aminoadenosine

8-Aminoadenosine (Synonyms: 8-NH2-Ado)

Cat. No.: HY-125927
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8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibits mRNA synthesis. 8-Aminoadenosine blocks Akt/mTOR signaling and induces autophagy and apoptosis in a p53-independent manner. 8-Aminoadenosine has antitumor activity.

For research use only. We do not sell to patients.

8-Aminoadenosine Chemical Structure

8-Aminoadenosine Chemical Structure

CAS No. : 3868-33-5

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Description

8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibits mRNA synthesis. 8-Aminoadenosine blocks Akt/mTOR signaling and induces autophagy and apoptosis in a p53-independent manner. 8-Aminoadenosine has antitumor activity[1][2][3].

IC50 & Target[1][2]

Akt

 

mTOR

 

In Vitro

8-Aminoadenosine (8-NH2-Ado; 0.1-10 μM; for 48 h) has IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively[1].
8-Aminoadenosine (10 μM; for 24 h) induces significant apoptotic death of MCF-7 cells in p53-independent pathway. 8-Aminoadenosine causes PARP cleavage in MCF-7 cells[2].
8-Aminoadenosine (3 μM; 0.5-4 h) induces autophagy in the MM.1S cell line[1].
8-Aminoadenosine (3 μM; 2-16 h) causes a greater drop in ATP levels in the MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) causes a 50% reduction in glucose consumption in MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) indicates a time-dependent decrease in GLUT1 expression at 5 h, whereas at 24 h there was a down-regulation of both transporters (GLUT1 and GLUT4) in MM.1S cells[1].
8-Aminoadenosine inhibits cell proliferation, activated cell death, and does not activate transcription of the p53 target gene p21 or increase protein levels of either p53 or p21[1].
The toxic effects of 8-Aminoadenosine require adenosine kinase activity to convert 8-Aminoadenosine to 8-NH2-ATP in adenosine kinase-deficient cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: MM.1S and U266 cells
Concentration: 0.1, 0.3, 1, 3, 10 μM
Incubation Time: For 48 hours
Result: Had IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively.

Apoptosis Analysis[2]

Cell Line: MCF-7 cells
Concentration: 10 μM
Incubation Time: For 24 hours
Result: Induced significant apoptotic death.
Apoptosis was not inhibited by knockdown of functional p53.

Apoptosis Analysis[1]

Cell Line: MM.1S cell line
Concentration: 3 μM
Incubation Time: 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 hours
Result: Induced the formation of LC3-II protein.
Caused the appearance of a population with a high AVO content with 1 μM for 24 hours.
Molecular Weight

282.26

Formula

C₁₀H₁₄N₆O₄

CAS No.
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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (295.22 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5428 mL 17.7142 mL 35.4283 mL
5 mM 0.7086 mL 3.5428 mL 7.0857 mL
10 mM 0.3543 mL 1.7714 mL 3.5428 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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8-Aminoadenosine
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HY-125927
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