2. Cell Cycle/DNA Damage PI3K/Akt/mTOR Autophagy Apoptosis
  3. DNA/RNA Synthesis Akt mTOR Autophagy Apoptosis
  4. 8-Aminoadenosine

8-Aminoadenosine  (Synonyms: 8-NH2-Ado)

Cat. No.: HY-125927 Purity: 99.94%
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8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibits mRNA synthesis. 8-Aminoadenosine blocks Akt/mTOR signaling and induces autophagy and apoptosis in a p53-independent manner. 8-Aminoadenosine has antitumor activity.

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8-Aminoadenosine Chemical Structure

8-Aminoadenosine Chemical Structure

CAS No. : 3868-33-5

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Based on 1 publication(s) in Google Scholar

8-Aminoadenosine Related Antibodies

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Description

8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibits mRNA synthesis. 8-Aminoadenosine blocks Akt/mTOR signaling and induces autophagy and apoptosis in a p53-independent manner. 8-Aminoadenosine has antitumor activity[1][2][3].

IC50 & Target[1][2]

mTOR

 

In Vitro

8-Aminoadenosine (8-NH2-Ado; 0.1-10 μM; for 48 h) has IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively[1].
8-Aminoadenosine (10 μM; for 24 h) induces significant apoptotic death of MCF-7 cells in p53-independent pathway. 8-Aminoadenosine causes PARP cleavage in MCF-7 cells[2].
8-Aminoadenosine (3 μM; 0.5-4 h) induces autophagy in the MM.1S cell line[1].
8-Aminoadenosine (3 μM; 2-16 h) causes a greater drop in ATP levels in the MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) causes a 50% reduction in glucose consumption in MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) indicates a time-dependent decrease in GLUT1 expression at 5 h, whereas at 24 h there was a down-regulation of both transporters (GLUT1 and GLUT4) in MM.1S cells[1].
8-Aminoadenosine inhibits cell proliferation, activated cell death, and does not activate transcription of the p53 target gene p21 or increase protein levels of either p53 or p21[1].
The toxic effects of 8-Aminoadenosine require adenosine kinase activity to convert 8-Aminoadenosine to 8-NH2-ATP in adenosine kinase-deficient cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8-Aminoadenosine Related Antibodies

Cell Viability Assay[1]

Cell Line: MM.1S and U266 cells
Concentration: 0.1, 0.3, 1, 3, 10 μM
Incubation Time: For 48 hours
Result: Had IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively.

Apoptosis Analysis[2]

Cell Line: MCF-7 cells
Concentration: 10 μM
Incubation Time: For 24 hours
Result: Induced significant apoptotic death.
Apoptosis was not inhibited by knockdown of functional p53.

Apoptosis Analysis[1]

Cell Line: MM.1S cell line
Concentration: 3 μM
Incubation Time: 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 hours
Result: Induced the formation of LC3-II protein.
Caused the appearance of a population with a high AVO content with 1 μM for 24 hours.
Molecular Weight

282.26

Formula

C10H14N6O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2N)O[C@@H]1CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (295.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5428 mL 17.7142 mL 35.4283 mL
5 mM 0.7086 mL 3.5428 mL 7.0857 mL
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* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5428 mL 17.7142 mL 35.4283 mL 88.5708 mL
5 mM 0.7086 mL 3.5428 mL 7.0857 mL 17.7142 mL
10 mM 0.3543 mL 1.7714 mL 3.5428 mL 8.8571 mL
15 mM 0.2362 mL 1.1809 mL 2.3619 mL 5.9047 mL
20 mM 0.1771 mL 0.8857 mL 1.7714 mL 4.4285 mL
25 mM 0.1417 mL 0.7086 mL 1.4171 mL 3.5428 mL
30 mM 0.1181 mL 0.5905 mL 1.1809 mL 2.9524 mL
40 mM 0.0886 mL 0.4429 mL 0.8857 mL 2.2143 mL
50 mM 0.0709 mL 0.3543 mL 0.7086 mL 1.7714 mL
60 mM 0.0590 mL 0.2952 mL 0.5905 mL 1.4762 mL
80 mM 0.0443 mL 0.2214 mL 0.4429 mL 1.1071 mL
100 mM 0.0354 mL 0.1771 mL 0.3543 mL 0.8857 mL
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8-Aminoadenosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Aminoadenosine3868-33-58-NH2-AdoDNA/RNA SynthesisAktmTORAutophagyApoptosisPKBProtein kinase BMammalian target of RapamycinRNAnucleosideATPp53MM.1SU266MCF-7Inhibitorinhibitorinhibit

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