Cat. No.: HY-125927

8-Aminoadenosine

(Synonyms: 8-NH2-Ado)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibits mRNA synthesis. 8-Aminoadenosine blocks Akt/mTOR signaling and induces autophagy and apoptosis in a p53-independent manner. 8-Aminoadenosine has antitumor activity.

For research use only. We do not sell to patients.

Purity: 99.05%
CAS No.: 3868-33-5
Formula: C₁₀H₁₄N₆O₄
Molecular Weight:282.26

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

8-Aminoadenosine Chemical Structure

CAS No.: 3868-33-5

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

mTOR

All DNA/RNA Synthesis Isoforms :

All Akt Isoforms :

All mTOR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	GI₅₀	0.05 μM Compound: 4	Cytotoxicity against human HCT116 cells by SRB assay Cytotoxicity against human HCT116 cells by SRB assay	[PMID: 19256508]
HCT-116	GI₅₀	0.05 μM Compound: 2	Antiproliferative activity against human HCT116 cells Antiproliferative activity against human HCT116 cells	[PMID: 21526763]

In Vitro

8-Aminoadenosine (8-NH2-Ado; 0.1-10 μM; for 48 h) has IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively^[1].
8-Aminoadenosine (10 μM; for 24 h) induces significant apoptotic death of MCF-7 cells in p53-independent pathway. 8-Aminoadenosine causes PARP cleavage in MCF-7 cells^[2].
8-Aminoadenosine (3 μM; 0.5-4 h) induces autophagy in the MM.1S cell line^[1].
8-Aminoadenosine (3 μM; 2-16 h) causes a greater drop in ATP levels in the MM.1S cells^[1].
8-Aminoadenosine (3 μM; 5 h) causes a 50% reduction in glucose consumption in MM.1S cells^[1].
8-Aminoadenosine (3 μM; 5 h) indicates a time-dependent decrease in GLUT1 expression at 5 h, whereas at 24 h there was a down-regulation of both transporters (GLUT1 and GLUT4) in MM.1S cells^[1].
8-Aminoadenosine inhibits cell proliferation, activated cell death, and does not activate transcription of the p53 target gene p21 or increase protein levels of either p53 or p21^[1].
The toxic effects of 8-Aminoadenosine require adenosine kinase activity to convert 8-Aminoadenosine to 8-NH2-ATP in adenosine kinase-deficient cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MM.1S and U266 cells
Concentration:

0.1, 0.3, 1, 3, 10 μM
Incubation Time:

For 48 hours
Result:

Had IC₅₀s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively.

Apoptosis Analysis^[2]

Cell Line:

MCF-7 cells
Concentration:

10 μM
Incubation Time:

For 24 hours
Result:

Induced significant apoptotic death.
Apoptosis was not inhibited by knockdown of functional p53.

Apoptosis Analysis^[1]

Cell Line:

MM.1S cell line
Concentration:

3 μM
Incubation Time:

0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 hours
Result:

Induced the formation of LC3-II protein.
Caused the appearance of a population with a high AVO content with 1 μM for 24 hours.

Chemical Information

CAS No. 3868-33-5
Appearance Solid
Molecular Weight 282.26
Formula C₁₀H₁₄N₆O₄
Color White to off-white
SMILES

O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2N)O[C@@H]1CO
Synonyms

8-NH2-Ado
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

bioRxiv

2024 July 27.

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (295.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5428 mL

17.7142 mL

35.4283 mL

5 mM

0.7086 mL

3.5428 mL

7.0857 mL

10 mM

0.3543 mL

1.7714 mL

3.5428 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mala Shanmugam, et al. Targeting glucose consumption and autophagy in myeloma with the novel nucleoside analogue 8-aminoadenosine. J Biol Chem. 2009 Sep 25;284(39):26816-30. [Content Brief]

[2]. Alla Polotskaia, et al. 8-Amino-adenosine activates p53-independent cell death of metastatic breast cancers. Mol Cancer Ther. 2012 Nov;11(11):2495-504. [Content Brief]

[3]. Jennifer Ann Frey, et al. 8-Amino-adenosine inhibits multiple mechanisms of transcription. Mol Cancer Ther. 2010 Jan;9(1):236-45. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5428 mL	17.7142 mL	35.4283 mL	88.5708 mL
	5 mM	0.7086 mL	3.5428 mL	7.0857 mL	17.7142 mL
	10 mM	0.3543 mL	1.7714 mL	3.5428 mL	8.8571 mL
	15 mM	0.2362 mL	1.1809 mL	2.3619 mL	5.9047 mL
	20 mM	0.1771 mL	0.8857 mL	1.7714 mL	4.4285 mL
	25 mM	0.1417 mL	0.7086 mL	1.4171 mL	3.5428 mL
	30 mM	0.1181 mL	0.5905 mL	1.1809 mL	2.9524 mL
	40 mM	0.0886 mL	0.4429 mL	0.8857 mL	2.2143 mL
	50 mM	0.0709 mL	0.3543 mL	0.7086 mL	1.7714 mL
	60 mM	0.0590 mL	0.2952 mL	0.5905 mL	1.4762 mL
	80 mM	0.0443 mL	0.2214 mL	0.4429 mL	1.1071 mL
	100 mM	0.0354 mL	0.1771 mL	0.3543 mL	0.8857 mL

Keywords

8-Aminoadenosine

1 Cited Publications

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8-Aminoadenosine

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

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Nucleotide Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

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Publications Citing Use of MedChemExpress (MCE) 8-Aminoadenosine

All DNA/RNA Synthesis Isoforms

All Akt Isoforms

All mTOR Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

8-Aminoadenosine Related Classifications

HY-125927 Related Classifications