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  5. Adenine

Adenine (6-Aminopurine), a purine, is one of the four nucleobases in the nucleic acid of DNA. Adenine acts as a chemical component of DNA and RNA. Adenine also plays an important role in biochemistry involved in cellular respiration, the form of both ATP and the cofactors (NAD and FAD), and protein synthesis.

For research use only. We do not sell to patients.

CAS No. : 73-24-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
25 g In-stock
50 g   Get quote  

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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of Adenine:

Adenine Related Antibodies

Top Publications Citing Use of Products

    Adenine purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin (10 and 15mg/kg, i.p.,once per week for 8 weeks) treatment lessened the deposition of ECM proteins in the kidneys from Adenine (0.25% w/w)-fed mice. Representative images of MTS (blue color), Picrosirius Red staining (red color), and IF staining of fibronectin (red color) and COL1A1 (green color) for kidney sections from adenine-fed (Adenine) or normal-fed (Ctrl) mice. Scale bars are 50 μm.

    Adenine purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin (10 and 15 mg/kg, i.p., once per week for 8 weeks) treatment lessened the deposition of ECM proteins in the kidneys from Adenine (0.25% w/w)-fed mice. WB images of fibronectin and COL1A1 expressions in kidney lysates from mice.

    Adenine purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin(10 or 15 mg/kg, i.p., once per week for 8 weeks) improved survival rate of Adenine (0.25% w/w)-fed mice. (A) Relative weight curves of indicated mice as described in A (n = 6). Mouse weight is measured once per 3 days, the initial weight is set to 1, and day 0 means the time point starting Adenine-rich diet. (B) Kaplan-Meier survival analysis of indicated mice as described in A (n = 12). Data are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

    Adenine purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin(10 or 15 mg/kg, i.p., once per week for 8 weeks) improved kidney function of Adenine (0.25% w/w)-fed mice. Concentrations of serum creatinine, urea, and albumin from mice (n = 8). Data are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

    Adenine purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin(10 or 15 mg/kg, i.p., once per week for 8 weeks) treatment prevented phenotypic alteration of tubular epithelial cells in the kidneys from Adenine (ADE) (0.25% w/w)-fed mice. Representative IHC staining images of E-cadherin and vimentin kidney sections from mice. Scale bars are 25 μm.

    View All DNA/RNA Synthesis Isoform Specific Products:

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    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Adenine (6-Aminopurine), a purine, is one of the four nucleobases in the nucleic acid of DNA. Adenine acts as a chemical component of DNA and RNA. Adenine also plays an important role in biochemistry involved in cellular respiration, the form of both ATP and the cofactors (NAD and FAD), and protein synthesis[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    A-431 IC50
    > 100 μg/mL
    Compound: adenine
    Inhibition of phosphatidylinositol 4-kinase in human A431 cell membrane by liquid scintillation counting
    Inhibition of phosphatidylinositol 4-kinase in human A431 cell membrane by liquid scintillation counting
    		[PMID: 1666120]
    MT4 IC50
    12 μM
    Compound: 2',3'-didehydro 2030-dideoxy adenine
    Potency against non nucleoside reverse transcriptor was determined in MT-4 cells
    Potency against non nucleoside reverse transcriptor was determined in MT-4 cells
    		[PMID: 15109677]
    Oocyte IC50
    200 μM
    Compound: 1
    Inhibitory activity against purified cdc2 p34/Cyclin B obtained from M phase oocytes of the starfish Marthasterias glacialis.
    Inhibitory activity against purified cdc2 p34/Cyclin B obtained from M phase oocytes of the starfish Marthasterias glacialis.
    		[PMID: 9046330]
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Adenine can be used to induce chronic kidney injury models. Pharmacokinetic data show that in adult male Sprague-Dawley rats, 20-30 minutes after administration (10 mg/kg, i.v.), the Adenine content in blood dialysate samples is 0.78 μg/mL[4].

    Induction of Chronic Kidney Disease (CKD)[4][5][6]
    Background
    Adenine is metabolized by the liver to form dihydroxyadenine, which is insoluble in water. The latter is deposited in the kidneys, which can cause post-renal obstruction, affect uric acid excretion, and cause kidney damage.
    Specific Modeling Methods
    Mice: C57BL/6J • 8 weeks of age
    Administration: 0.2% Adenine in diet; 3 weeks
    Rat: Sprague-Dawley (SD) • male • 8 weeks of age
    Administration: 0.5% Adenine in diet; 3 weeks
    Note
    Modeling Indicators
    Biochemical changs: KW-to-BW ratio increasing; Systolic and diastotic blood pressure increasing; Blood urea nitrogen increasing; serum creatinine levels increasing

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    135.13

    Formula

    C5H5N5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=C2N=CNC2=NC=N1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 10 mg/mL (74.00 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2 mg/mL (14.80 mM; ultrasonic and warming and heat to 60°C)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.4003 mL 37.0014 mL 74.0028 mL
    5 mM 1.4801 mL 7.4003 mL 14.8006 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 1 mg/mL (7.40 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.99%

    SDS (480 KB)

    COA (254 KB) HNMR (129 KB) RP-HPLC (213 KB) LCMS (317 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 7.4003 mL 37.0014 mL 74.0028 mL 185.0070 mL
    5 mM 1.4801 mL 7.4003 mL 14.8006 mL 37.0014 mL
    10 mM 0.7400 mL 3.7001 mL 7.4003 mL 18.5007 mL
    DMSO 15 mM 0.4934 mL 2.4668 mL 4.9335 mL 12.3338 mL
    20 mM 0.3700 mL 1.8501 mL 3.7001 mL 9.2504 mL
    25 mM 0.2960 mL 1.4801 mL 2.9601 mL 7.4003 mL
    30 mM 0.2467 mL 1.2334 mL 2.4668 mL 6.1669 mL
    40 mM 0.1850 mL 0.9250 mL 1.8501 mL 4.6252 mL
    50 mM 0.1480 mL 0.7400 mL 1.4801 mL 3.7001 mL
    60 mM 0.1233 mL 0.6167 mL 1.2334 mL 3.0835 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Adenine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Adenine73-24-5DNA/RNA SynthesisEndogenous MetabolitepurineDNAnucleobaseInhibitorinhibitorinhibit

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