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  5. Adenine hemisulfate

Adenine hemisulfate  (Synonyms: 6-Aminopurine hemisulfate; Vitamin B4 hemisulfate)

Cat. No.: HY-B0152B Purity: 99.95%
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Adenine hemisulfate (6-Aminopurine hemisulfate), a purine, is one of the four nucleobases in the nucleic acid of DNA. Adenine hemisulfate acts as a chemical component of DNA and RNA. Adenine hemisulfate also plays an important role in biochemistry involved in cellular respiration, the form of both ATP and the cofactors (NAD and FAD), and protein synthesis.

For research use only. We do not sell to patients.

CAS No. : 321-30-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 g In-stock
5 g   Get quote  
10 g   Get quote  

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Based on 22 publication(s) in Google Scholar

Other Forms of Adenine hemisulfate:

Adenine hemisulfate Related Antibodies

Top Publications Citing Use of Products

    Adenine hemisulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin (10 and 15mg/kg, i.p.,once per week for 8 weeks) treatment lessened the deposition of ECM proteins in the kidneys from Adenine (0.25% w/w)-fed mice. Representative images of MTS (blue color), Picrosirius Red staining (red color), and IF staining of fibronectin (red color) and COL1A1 (green color) for kidney sections from adenine-fed (Adenine) or normal-fed (Ctrl) mice. Scale bars are 50 μm.

    Adenine hemisulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin (10 and 15 mg/kg, i.p., once per week for 8 weeks) treatment lessened the deposition of ECM proteins in the kidneys from Adenine (0.25% w/w)-fed mice. WB images of fibronectin and COL1A1 expressions in kidney lysates from mice.

    Adenine hemisulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin(10 or 15 mg/kg, i.p., once per week for 8 weeks) improved survival rate of Adenine (0.25% w/w)-fed mice. (A) Relative weight curves of indicated mice as described in A (n = 6). Mouse weight is measured once per 3 days, the initial weight is set to 1, and day 0 means the time point starting Adenine-rich diet. (B) Kaplan-Meier survival analysis of indicated mice as described in A (n = 12). Data are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

    Adenine hemisulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin(10 or 15 mg/kg, i.p., once per week for 8 weeks) improved kidney function of Adenine (0.25% w/w)-fed mice. Concentrations of serum creatinine, urea, and albumin from mice (n = 8). Data are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

    Adenine hemisulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Mar 21;100:154067.  [Abstract]

    Capsaicin(10 or 15 mg/kg, i.p., once per week for 8 weeks) treatment prevented phenotypic alteration of tubular epithelial cells in the kidneys from Adenine (ADE) (0.25% w/w)-fed mice. Representative IHC staining images of E-cadherin and vimentin kidney sections from mice. Scale bars are 25 μm.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Adenine hemisulfate (6-Aminopurine hemisulfate), a purine, is one of the four nucleobases in the nucleic acid of DNA. Adenine hemisulfate acts as a chemical component of DNA and RNA. Adenine hemisulfate also plays an important role in biochemistry involved in cellular respiration, the form of both ATP and the cofactors (NAD and FAD), and protein synthesis[1][2].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Adenine hemisulfate can be used to induce chronic liver injury models. Pharmacokinetic data show that in adult male Sprague-Dawley rats, 20-30 minutes after administration (10 mg/kg, i.v.), the Adenine content in blood dialysate samples is 0.78 μg/mL[4].

    Induction of Chronic Kidney Disease (CKD)[4][5][6]
    Background
    Adenine is metabolized by the liver to form dihydroxyadenine, which is insoluble in water. The latter is deposited in the kidneys, which can cause post-renal obstruction, affect uric acid excretion, and cause kidney damage.
    Specific Modeling Methods
    Mice: C57BL/6J • 8 weeks of age
    Administration: 0.2% Adenine in diet; 3 weeks
    Rat: Sprague-Dawley (SD) • male • 8 weeks of age
    Administration: 0.5% Adenine in diet; 3 weeks
    Note
    Modeling Indicators
    Biochemical changs: KW-to-BW ratio increasing; Systolic and diastotic blood pressure increasing; Blood urea nitrogen increasing; serum creatinine levels increasing

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    184.17

    Formula

    C5H5N5.1/2H2SO4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=C2N=CNC2=NC=N1.O=S(O)(O)=O.[1/2]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (542.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (27.15 mM; ultrasonic and heat to 50°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.4298 mL 27.1488 mL 54.2977 mL
    5 mM 1.0860 mL 5.4298 mL 10.8595 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (13.57 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (13.57 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 2 mg/mL (10.86 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.95%

    SDS (420 KB)

    COA (251 KB) HNMR (260 KB) RP-HPLC (205 KB) MS (230 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.4298 mL 27.1488 mL 54.2977 mL 135.7442 mL
    5 mM 1.0860 mL 5.4298 mL 10.8595 mL 27.1488 mL
    10 mM 0.5430 mL 2.7149 mL 5.4298 mL 13.5744 mL
    15 mM 0.3620 mL 1.8099 mL 3.6198 mL 9.0496 mL
    20 mM 0.2715 mL 1.3574 mL 2.7149 mL 6.7872 mL
    25 mM 0.2172 mL 1.0860 mL 2.1719 mL 5.4298 mL
    DMSO 30 mM 0.1810 mL 0.9050 mL 1.8099 mL 4.5248 mL
    40 mM 0.1357 mL 0.6787 mL 1.3574 mL 3.3936 mL
    50 mM 0.1086 mL 0.5430 mL 1.0860 mL 2.7149 mL
    60 mM 0.0905 mL 0.4525 mL 0.9050 mL 2.2624 mL
    80 mM 0.0679 mL 0.3394 mL 0.6787 mL 1.6968 mL
    100 mM 0.0543 mL 0.2715 mL 0.5430 mL 1.3574 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Adenine hemisulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Adenine hemisulfate321-30-26-Aminopurine hemisulfate Vitamin B4 hemisulfateDNA/RNA SynthesisEndogenous MetabolitepurineDNAnucleobaseInhibitorinhibitorinhibit

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