Guaiacol (Synonyms: 2-Methoxyphenol)

Name: Guaiacol
Brand: MedChemExpress
SKU: HY-N1380
Rating: 4.5 (0 reviews)

Cat. No.: HY-N1380 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Guaiacol, a phenolic compound, inhibits LPS-stimulated COX-2 expression and NF-κB activation. Anti-inflammatory activity.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Guaiacol 화학구조

CAS No. : 90-05-1

사이즈	재고	수량
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid or liquid
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	해외재고보유	Estimated Time of Arrival: December 31
25 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	해외재고보유	Estimated Time of Arrival: December 31
100 g	해외재고보유	Estimated Time of Arrival: December 31
> 100 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of Guaiacol:

Guaiacol-d₃ In-stock
Guaiacol (Standard) In-stock
Guaiacol-d₇ 견적 받기
Guaiacol-¹³C₆ 견적 받기
Guaiacol-d₄ 견적 받기
Guaiacol-d₄-1 견적 받기

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

View All Isoforms

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

View All COX Isoform Specific Products:

View All Isoforms

COX COX-1 COX-2 COX-3

Biological Activity
순도&문서
References
고객리뷰

제품 설명

Guaiacol, a phenolic compound, inhibits LPS-stimulated COX-2 expression and NF-κB activation^[1]. Anti-inflammatory activity^[1].

IC₅₀ & Target^[1]

COX-2

NF-κB

Cellular Effect

Cell Line	Type	Value	Description	References
U-251	EC₅₀	1141 3 Compound: 8	Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-251	EC₅₀	1141 3 Compound: 8	Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-87MG ATCC	EC₅₀	492 3 Compound: 8	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-87MG ATCC	EC₅₀	492 3 Compound: 8	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-251	EC₅₀	1141 3 Compound: 8	Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-87MG ATCC	EC₅₀	492 3 Compound: 8	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]

In Vitro

Guaiacol inhibits LPS-stimulated nuclear factor kappa B (NF-κB) activation and cyclooxygenase (COX)-2 gene expression in cells of the RAW 264.7 murine macrophage cell line. Phenolic compounds such as Phenol, Eugenol, Guaiacol and Vanillin inhibit sheep vesicular gland prostaglandin cyclooxygenase, as indicated by their 50% inhibition concentrations, which decline in the following order: Phenol (1600 μM) > Vanillin (>500 μM) > Guaiacol (72 μM) > Eugenol (12 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

분자량

124.14

화학식

C₇H₈O₂

CAS No.

90-05-1

Appearance

<26°C Solid,>29°C Liquid

Color

Colorless to off-white

SMILES

OC1=CC=CC=C1OC

Structure Classification

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : ≥ 100 mg/mL (805.54 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 37.5 mg/mL (302.08 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.0554 mL	40.2771 mL	80.5542 mL
5 mM	1.6111 mL	8.0554 mL	16.1108 mL
10 mM	0.8055 mL	4.0277 mL	8.0554 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (134.28 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.96%

Select Batch:

Data Sheet (276 KB) SDS (394 KB)

COA (253 KB) HNMR (129 KB) GC (244 KB)

Handling Instructions (2659 KB)

References

[1]. Murakami Y, et al. Re-evaluation of cyclooxygenase-2-inhibiting activity of vanillin and Guaiacol in macrophages stimulated with lipopolysaccharide. Anticancer Res. 2007 Mar-Apr;27(2):801-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.0554 mL	40.2771 mL	80.5542 mL	201.3855 mL
	5 mM	1.6111 mL	8.0554 mL	16.1108 mL	40.2771 mL
	10 mM	0.8055 mL	4.0277 mL	8.0554 mL	20.1386 mL
	15 mM	0.5370 mL	2.6851 mL	5.3703 mL	13.4257 mL
	20 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0693 mL
	25 mM	0.3222 mL	1.6111 mL	3.2222 mL	8.0554 mL
	30 mM	0.2685 mL	1.3426 mL	2.6851 mL	6.7129 mL
	40 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0346 mL
	50 mM	0.1611 mL	0.8055 mL	1.6111 mL	4.0277 mL
	60 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3564 mL
	80 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	100 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Guaiacol Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Guaiacol90-05-12-MethoxyphenolNF-κBEnvironmental PollutantsEndogenous MetaboliteCOXNuclear factor-κBNuclear factor-kappaBEnvironmental ContaminantsCyclooxygenaseInhibitorinhibitorinhibit

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Guaiacol (Synonyms: 2-Methoxyphenol)

고객리뷰

Other Forms of Guaiacol:

Guaiacol Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

View All COX Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

Guaiacol Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Guaiacol Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report