Lomefloxacin (hydrochloride)  (Synonyms: SC47111A (hydrochloride); ロメフロキサシン 塩酸塩)

Lomefloxacin hydrochloride (NY-198 hydrochloride) is an orally active difluoroquinolone antibiotic. Lomefloxacin hydrochloride prevents DNA supercoiling and replication by inhibiting bacterial topoisomerase II. Lomefloxacin hydrochloride induces ROS production and Apoptosis. Lomefloxacin hydrochloride has broad-spectrum bactericidal activity against Gram-positive and Gram-negative bacteria. Lomefloxacin hydrochloride has anticancer effects against melanoma. Lomefloxacin hydrochloride can be used in the study of systemic bacterial infections (such as Salmonella typhimurium infections), skin and melanoma ^{[1][2][3][4][5][6]}.

Lomefloxacin (1/4 to 2×MIC concentrations) hydrochloride rapidly reduces viable cell counts of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, with no regrowth observed in all strains except P. aeruginosa^[1].
Lomefloxacin hydrochloride exhibits MIC values ranging from 0.06-4 μg/mL in susceptibility determination experiments with strains such as Escherichia coli and Staphylococcus aureus^[2].
Lomefloxacin (0.1-1.0 mM; 24-72 h) hydrochloride decreases viability of COLO829 cells and induces ROS production in COLO829 cells^[3].
Lomefloxacin (50-100 μM) hydrochloride induces apoptosis in keratinocyte^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lomefloxacin (ED₅₀: 1.45-32.2 mg/kg; p.o.; immediately after infection) hydrochloride significantly reduces mortality in mice with intraperitoneal infection models, with ED₅₀ values lower than those of Ofloxacin and Norfloxacin^[1].
Lomefloxacin (-80 mg/kg; orogastric administration) hydrochloride reduces mortality, significantly decreases splenic bacterial counts, and inhibits inflammatory response in mice infected with Salmonella typhimurium^[5].
Lomefloxacin (16-32 mg/kg; i.p.; 5 days) hydrochloride only at the highest dose slightly reduces the number of implants and live fetuses in the third week of mating in mice, without significant dominant lethal mutation induction^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

387.81

C₁₇H₂₀ClF₂N₃O₃

98079-52-8

Solid

White to off-white

CCN(C=C(C(O)=O)C1=O)C(C1=CC(F)=C2N(CCN3)CC3C)=C2F.Cl

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Hirose T, et al. In vitro and in vivo activity of NY-198, a new difluorinated quinolone. Antimicrob Agents Chemother. 1987 Jun;31(6):854-9.  [Content Brief]

  [2]. Piddock LJ, et al. Mechanism of action of lomefloxacin. Antimicrob Agents Chemother. 1990 Jun;34(6):1088-93.  [Content Brief]

  [3]. Beberok A, et al. Lomefloxacin Induces Oxidative Stress and Apoptosis in COLO829 Melanoma Cells. Int J Mol Sci. 2017 Oct 20;18(10):2194.  [Content Brief]

  [4]. Marrot L, et al. Molecular responses to photogenotoxic stress induced by the antibiotic lomefloxacin in human skin cells: from DNA damage to apoptosis. J Invest Dermatol. 2003 Sep;121(3):596-606.  [Content Brief]

  [5]. Butler T, et al. Treatment of experimental Salmonella typhimurium infection in mice with lomefloxacin. J Antimicrob Chemother. 1990 Apr;25(4):629-34.  [Content Brief]

  [6]. Singh AC, et al. Genotoxicity of lomefloxacin--an antibacterial drug in somatic and germ cells of Swiss albino mice in vivo. Mutat Res. 2003 Feb 5;535(1):35-42.  [Content Brief]