2. Epigenetics PI3K/Akt/mTOR Stem Cell/Wnt
  3. AMPK Wnt
  4. Platycodin D

Platycodin D  (Synonyms: プラチコジンD)

製品番号: HY-N1411 純度: 99.34%
COA 取扱説明書 Technical Support

Platycodin D is a saponin isolated from Platycodon grandiflorus, acts as an activator of AMPKα, with anti-obesity property. WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D.

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CAS 番号 : 58479-68-8

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 141 在庫あり
Solution
10 mM * 1 mL in DMSO USD 141 在庫あり
Solid
5 mg $66 在庫あり
10 mg $105 在庫あり
25 mg $189 在庫あり
50 mg $292 在庫あり
100 mg $445 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 8 publication(s) in Google Scholar

Other Forms of Platycodin D:

Platycodin D 関連抗体

Top Publications Citing Use of Products

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb:115:109733.

    Platycodin D (PlaD; 10, 20 mg/kg; i.g; once daily for 23 days) significantly inhibited tumor growth (fig B).The tumor inhibition rate is approximately 39 % in response to 10 mg/kg and 57 % in response to 20 mg/kg (fig C).

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb:115:109733.

    Platycodin D (PlaD; 3.75, 5, 7.5, 10, 15, 20 μM; 24 h) dose-dependently inhibits the viability of dHL-60 cells.

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb:115:109733.

    When the dHL-60 cells are treated with Platycodin D (PlaD; 0, 4, 8, 12 μM; 24 h), the numbers of migrated cells decreases significantly in a dose-dependent manner with or without IFN-γ induction.

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb:115:109733.

    Platycodin D (PlaD; 4, 8, 12 μM) inhibits the high expression of PD-L1 in dHL-60 cells induced by IFN-γ (Fig. F and G).

    AMPK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1 BRSK1 BRSK2 HUNK AMPKα

    Wnt アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Wnt1 Wnt3 Wnt5 Wnt7 Wnt11

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Platycodin D is a saponin isolated from Platycodon grandiflorus, acts as an activator of AMPKα, with anti-obesity property. WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D[1][2].

    IC50 & Target

    AMPKα[1]
    Cellular Effect
    Cell Line Type Value Description References
    HCT-15 IC50
    5.7 μM
    Compound: 5
    Antiproliferative activity against human HCT15 cells after 48 hrs by sulforhodamine B assay
    Antiproliferative activity against human HCT15 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 20939516]
    HCT-15 IC50
    7.9 μM
    Compound: 5
    Antiproliferative activity against human HCT15/CL02 cells after 48 hrs by sulforhodamine B assay
    Antiproliferative activity against human HCT15/CL02 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 20939516]
    HSC-T6 IC50
    1.77 μM
    Compound: 147
    Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 29353722]
    MES-SA IC50
    3.9 μM
    Compound: 5
    Antiproliferative activity against human MESSA cells after 48 hrs by sulforhodamine B assay
    Antiproliferative activity against human MESSA cells after 48 hrs by sulforhodamine B assay
    		[PMID: 20939516]
    MES-SA/Dx5 IC50
    >10 μM
    Compound: 5
    Antiproliferative activity against human MESSA/DX5 cells after 48 hrs by sulforhodamine B assay
    Antiproliferative activity against human MESSA/DX5 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 20939516]
    体外実験

    Platycodin D (4 μM; 24 h) increases gene expressions of Ucp1, Pgc1a, Sirt3, Cytc, Nrf1 and Prdm16, and suppresses expressions of adipokine genes Fabp4, Adipoq, Lipin1 in 3T3-L1 adipocytes and FABP4, ADIPOQ and RESISTIN in hAMSCs[1].
    Platycodin D (2 and 10 μM) suppresses lipid accumulation in murine and human adipocytes respectively[1].
    Platycodin D suppresses the protein expressions of PPARγ and C/EBPα, and increases protein expressions of UCP1, PGC1α, SIRT3, CIDEA and LIPIN1[1].
    Platycodin D (10 μM) inhibits lipid droplet formation, and reduces the expression of the FABP4 protein involved in lipid metabolism and the expressions of PPARγ and C/EBPα[2].
    Platycodin D (10 μM; 0-7 d) recoveres CCND1 mRNA level and increases PPARδ level[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Platycodin D 関連抗体
    体内実験

    Platycodin D (5 mg/kg; oral administration, 5 times per week for 5 weeks) decreases the body weight of obese mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Genetically obese db/db mice[1]
    Dosage: 5 mg/kg
    Administration: Oral administration; 5 mg/kg, 5 times per week for 5 weeks
    Result: Significantly decreased body weight gain without affecting food intake of mice. Decreased the liver tissue weight and increased the weight of brown adipose tissue (BAT).
    分子量

    1225.32

    分子式

    C57H92O28
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C([C@]1(CCC(C)(C)C2)[C@]2([H])C3=CC[C@@]4([H])[C@@](C)(CC[C@]5([H])[C@@]4(C[C@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]6O)O)([H])O[C@@H]6CO)C5(CO)CO)C)[C@]3(C)C[C@H]1O)O[C@H](OC[C@H](O)[C@@H]7O)[C@@H]7O[C@@](O[C@@H](C)[C@H](O[C@@](OC[C@@H](O)[C@@H]8O[C@@](OC[C@]9(O)CO)([H])[C@@H]9O)([H])[C@@H]8O)[C@H]%10O)([H])[C@@H]%10O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (40.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.8161 mL 4.0806 mL 8.1611 mL
    5 mM 0.1632 mL 0.8161 mL 1.6322 mL
    10 mM 0.0816 mL 0.4081 mL 0.8161 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (2.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (2.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 20 mg/mL (16.32 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 20 mg/mL (16.32 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.34%

    COA (254 KB) RP-HPLC (250 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.8161 mL 4.0806 mL 8.1611 mL 20.4028 mL
    5 mM 0.1632 mL 0.8161 mL 1.6322 mL 4.0806 mL
    10 mM 0.0816 mL 0.4081 mL 0.8161 mL 2.0403 mL
    15 mM 0.0544 mL 0.2720 mL 0.5441 mL 1.3602 mL
    20 mM 0.0408 mL 0.2040 mL 0.4081 mL 1.0201 mL
    25 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8161 mL
    30 mM 0.0272 mL 0.1360 mL 0.2720 mL 0.6801 mL
    40 mM 0.0204 mL 0.1020 mL 0.2040 mL 0.5101 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Platycodin D Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Platycodin D58479-68-8AMPKWntAMP-activated protein kinasesaponinanti-obesityanti-adipogenicadipokinehAMSClipidmiceInhibitorinhibitorinhibit

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    製品名:
    Platycodin D
    製品番号:
    HY-N1411
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