2. Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK JAK/STAT Signaling Epigenetics Stem Cell/Wnt Autophagy Apoptosis NF-κB Immunology/Inflammation
  3. Aldose Reductase JAK STAT Autophagy Apoptosis NF-κB SOD Reactive Oxygen Species (ROS)
  4. Zingiberen newsaponin

Zingiberen newsaponin  (Synonyms: Zingiberensis newsaponin)

製品番号: HY-N2282 純度: 99.68%
COA 取扱説明書 Technical Support

Zingiberen Newsaponin (Zingiberensis newsaponin) is an orally active type of steroid saponin compound. Zingiberen Newsaponin exerts anti-hepatocellular carcinoma (HCC) effects by inhibiting autophagy and the AKR1C1/JAK2/STAT3 pathway. Zingiberen Newsaponin activates oxidative stress (upregulates ROS and MDA) and mitochondrial pathways, promoting cancer cell apoptosis. Zingiberen Newsaponin alleviates cerebral ischemia-reperfusion (I/R) injury by decreasing the concentration of pro-inflammatory cytokines and inhibits NF-κB. Zingiberen Newsaponin can enhance the activity of SOD, eliminate free radicals and protect nerve cells. Zingiberen Newsaponin induces platelet aggregation.

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Zingiberen newsaponin

Zingiberen newsaponin 構造式

CAS 番号 : 91653-50-8

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 518 在庫あり
Solution
10 mM * 1 mL in DMSO USD 518 在庫あり
Solid
5 mg $280 在庫あり
10 mg $450 在庫あり
25 mg $700 在庫あり
50 mg $980 在庫あり
100 mg   お問い合わせ  
200 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Zingiberen newsaponin 関連抗体

Top Publications Citing Use of Products

JAK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
JAK1 JAK2 JAK3 Tyk2 JAK

STAT アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
STAT3 STAT5 STAT6 STAT1

NF-κB アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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製品説明

Zingiberen Newsaponin (Zingiberensis newsaponin) is an orally active type of steroid saponin compound. Zingiberen Newsaponin exerts anti-hepatocellular carcinoma (HCC) effects by inhibiting autophagy and the AKR1C1/JAK2/STAT3 pathway. Zingiberen Newsaponin activates oxidative stress (upregulates ROS and MDA) and mitochondrial pathways, promoting cancer cell apoptosis. Zingiberen Newsaponin alleviates cerebral ischemia-reperfusion (I/R) injury by decreasing the concentration of pro-inflammatory cytokines and inhibits NF-κB. Zingiberen Newsaponin can enhance the activity of SOD, eliminate free radicals and protect nerve cells. Zingiberen Newsaponin induces platelet aggregation[1][2][3].

体外実験

Zingiberen Newsaponin (0-2 μM, 48 h) inhibits proliferation of Huh7 and SMMC-7721 cells, with IC50 values of 443.8 nM and 841.8 nM[2].
Zingiberen Newsaponin (0.5-1 μM, 24-48 h) significantly inhibits the migration of Huh7 and SMMC-7721 cells and induces cell apoptosis[2].
Zingiberen Newsaponin (0.5-1 μM, 48 h) inhibits AKR1C1/JAK2/STAT3 signaling pathway, induces oxidative stress and inhibits autophagy in Huh7 and SMMC-7721 cells[2].
Zingiberen Newsaponin (48 h) significantly reduces the secretion of pro-inflammatory cytokines TNF-α and IL-6 and downregulates NF-κB protein expression in LPS (HY-D1056)-stimulated RAW264.7 cells[3].
Zingiberen Newsaponin (48 h) significantly improves PC12 cell survival and reduces apoptosis in an ischemia-reperfusion (I/R) cell model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Zingiberen newsaponin 関連抗体

Cell Migration Assay [2]

Cell Line: Huh7 and SMMC-7721 cells
Concentration: 0.5 μM (Huh7) and 1 μM (SMMC-7721)
Incubation Time: 24 h
Result: Significantly inhibited the migration of HCC cells.

Apoptosis Analysis[2]

Cell Line: Huh7 and SMMC-7721 cells
Concentration: 0.5 μM (Huh7) and 1 μM (SMMC-7721)
Incubation Time: 48 h
Result: Significantly promotes HCC cell apoptosis.

ELISA Assay[2]

Cell Line: Huh7 and SMMC-7721 cells
Concentration: 0.5 μM (Huh7) and 1 μM (SMMC-7721)
Incubation Time: 48 h
Result: Significantly increased ROS, SOD, and MDA levels in HCC cells.

Western Blot Analysis[2]

Cell Line: Huh7 and SMMC-7721 cells
Concentration: 0.5 μM (Huh7) and 1 μM (SMMC-7721)
Incubation Time: 48 h
Result: Downregulated AKR1C1, p-JAK2, and p-STAT3 expression.
体内実験

Zingiberen Newsaponin (60 mg/kg, p.o., once daily for 28 days) significantly inhibits tumor growth in a mouse HCC xenograft model[2].
Zingiberen Newsaponin (i.g., once daily for 7 days or single dose) significantly improves cerebral I/R injury, reducing infarct size and neurological deficits in rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: SMMC-7721 xenograft model model established in BALB/c nude mice (4 weeks old, male)[2]
Dosage: 60 mg/kg
Administration: Oral administration (p.o.), once daily for 28 days
Result: Significantly inhibited tumor growth, reducing tumor volume and weight.
Decreased Ki67 (a proliferation marker) expression and promoted tumor cell apoptosis.
分子量

1047.18

分子式

C51H82O22
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@@H](O[C@@](O[C@H](CO)[C@@H](O)[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)([H])[C@@H]7O)[C@@H]9O)([H])[C@@H]9O[C@@](O[C@@H](C)[C@H](O)[C@H]%10O)([H])[C@@H]%10O)C5)C)([H])[C@]6([H])CC2
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (95.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9549 mL 4.7747 mL 9.5495 mL
5 mM 0.1910 mL 0.9549 mL 1.9099 mL
10 mM 0.0955 mL 0.4775 mL 0.9549 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (2.39 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.68%

COA (254 KB) HNMR (153 KB) CNMR (180 KB) RP-HPLC (190 KB) MS (157 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9549 mL 4.7747 mL 9.5495 mL 23.8736 mL
5 mM 0.1910 mL 0.9549 mL 1.9099 mL 4.7747 mL
10 mM 0.0955 mL 0.4775 mL 0.9549 mL 2.3874 mL
15 mM 0.0637 mL 0.3183 mL 0.6366 mL 1.5916 mL
20 mM 0.0477 mL 0.2387 mL 0.4775 mL 1.1937 mL
25 mM 0.0382 mL 0.1910 mL 0.3820 mL 0.9549 mL
30 mM 0.0318 mL 0.1592 mL 0.3183 mL 0.7958 mL
40 mM 0.0239 mL 0.1194 mL 0.2387 mL 0.5968 mL
50 mM 0.0191 mL 0.0955 mL 0.1910 mL 0.4775 mL
60 mM 0.0159 mL 0.0796 mL 0.1592 mL 0.3979 mL
80 mM 0.0119 mL 0.0597 mL 0.1194 mL 0.2984 mL
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Zingiberen newsaponin Related Classifications

  • Molarity Calculator

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Zingiberen newsaponin91653-50-8Zingiberensis newsaponinAldose ReductaseJAKSTATAutophagyApoptosisNF-κBSODReactive Oxygen Species (ROS)Janus kinaseNuclear factor-κBNuclear factor-kappaBSuperoxide DismutaseHepatocellular carcinomaOxidative stress3-MethyladenineInhibitorinhibitorinhibit

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