4-DAMP (Synonyms: 4-DAMP methiodide)

Name: 4-DAMP
Brand: MedChemExpress
SKU: HY-100958
Rating: 5.0 (4 reviews)

製品番号: HY-100958 純度: 99.96%: Data Sheet SDS COA 取扱説明書
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4-DAMP (4-DAMP methiodide) is a potent and selective antagonist of M3 receptors and also has a high affinity for the closely-related M5 receptors. 4-DAMP combined with 5-Fluorouracil (5-Fu) (HY-90006) could significantly reduce the cell viability and enhance apoptosis in MKN45 and BGC823 gastric cancer cells. 4-DAMP inhibits lipopolysaccharide (LPS)- and tobacco-induced pulmonary inflammation and reduces mucin 5AC (MUC5AC), oligomeric mucus/gel-forming secretion.

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4-DAMP 構造式

CAS 番号 : 1952-15-4

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 44	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 44	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$40	在庫あり	Estimated Time of Arrival: December 31
10 mg	$60	在庫あり	Estimated Time of Arrival: December 31
25 mg	$110	在庫あり	Estimated Time of Arrival: December 31
50 mg	$170	在庫あり	Estimated Time of Arrival: December 31
100 mg	$255	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 4 publication(s) in Google Scholar

顧客検証

mAChR アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

MMP アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

EGFR アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

Interleukin Related アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

生物活性
純度とドキュメンテーション
参考文献
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製品説明

IC₅₀ & Target

mAChR3

mAChR5

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	4.1 × 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	3.2 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	3.2 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant muscarinic M5 receptor expressed in CHO cells Displacement of [3H]4-DAMP from human recombinant muscarinic M5 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	4.1 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	3.2 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	4.9 × 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	4.9 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	3.2 × 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M5 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	4.1 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant muscarinic M3 receptor expressed in CHO cells Displacement of [3H]4-DAMP from human recombinant muscarinic M3 receptor expressed in CHO cells	[PMID: 26988801]
CHO-K1	IC₅₀	0.055 3 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO	IC₅₀	4.1 × 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant muscarinic M3 receptor expressed in CHO cells Displacement of [3H]4-DAMP from human recombinant muscarinic M3 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	4.1 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M3 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO-K1	IC₅₀	4.5 1 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO	IC₅₀	4.9 × 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant muscarinic M4 receptor expressed in CHO cells Displacement of [3H]4-DAMP from human recombinant muscarinic M4 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	4.9 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant muscarinic M4 receptor expressed in CHO cells Displacement of [3H]4-DAMP from human recombinant muscarinic M4 receptor expressed in CHO cells	[PMID: 26988801]
CHO-K1	IC₅₀	5.1 1 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO	IC₅₀	3.2 × 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant muscarinic M5 receptor expressed in CHO cells Displacement of [3H]4-DAMP from human recombinant muscarinic M5 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	4.9 x 10^-10 41 Compound: 4-DAMP	Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]4-DAMP from human recombinant M4 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO-K1	IC₅₀	0.055 3 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	4.5 1 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	5.1 1 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	0.055 3 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M2 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	4.5 1 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M1 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	5.1 1 Compound: 4-DAMP	Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]N-methylscopolamine from human recombinant muscarinic M3 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]

体外実験

4-DAMP (10 μM, 5 days) combined with 5-Fu (5 μM, 5 days) can inhibit cell growth and promote apoptosis in MKN45 and BGC823 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	MKN45 and BGC823 cells
Concentration:	10 μM
Incubation Time:	5 days
Result:	Reduced the cell viability to (30±4.0)% and (35±6.0)% combined with 5-Fu in MKN45 and BGC823 cells, respectively.

体内実験

4-DAMP (1mg/kg, i.p., daily for 12 days) inhibits gastric tumor formation in nude mouse MKN45 and BGC823 tumor xenografts^[2].
4-DAMP (0.6 or 0.12 mg/kg, intranasal instillation, daily for 35 days) reduces the allergic rhinitis (AR) symptom scores and nasal secretion weights, reverses goblet cell metaplasia, alleviates eosinophil infiltration, inhibits the levels of MUC5AC, histamine and cytokines, MMP9, EGFR in AR guinea pigs^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mouse MKN45 and BGC823 tumor xenografts^[2]
Dosage:	1mg/kg
Administration:	i.p., daily for 12 days
Result:	Reduced the tumor sizes and the tumor weights in both MKN45 and BGC823 xenografts.

Animal Model:	AR guinea pigs^[3]
Dosage:	0.6 or 0.12 mg/kg
Administration:	intranasal instillation, daily for 35 days
Result:	Reduced the weights of nasal secretions, reversed goblet cell metaplasia of the animal nasal mucosal epithelium, inhibited the levels of MUC5AC mRNA, IL-4, IL-13, and TNF-α in AR guinea pigs.

分子量

451.34

分子式

C₂₁H₂₆INO₂

CAS 番号

1952-15-4

Appearance

Solid

Color

White to off-white

SMILES

C[N+]1(C)CCC(OC(C(C2=CC=CC=C2)C3=CC=CC=C3)=O)CC1.[I-]

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (221.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2156 mL	11.0781 mL	22.1562 mL
5 mM	0.4431 mL	2.2156 mL	4.4312 mL
10 mM	0.2216 mL	1.1078 mL	2.2156 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.08 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (11.08 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.96%

Select Batch:

データシート (279 KB) SDS (394 KB)

COA (250 KB) HNMR (244 KB) RP-HPLC (242 KB) MS (69 KB) Elemental Analysis Report (108 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Watson N, et al. Comparative pharmacology of recombinant human M3 and M5 muscarinic receptors expressed in CHO-K1 cells. Br J Pharmacol. 1999 May;127(2):590-6. [Content Brief]

[2]. Yu H, et al. Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation. Sci Rep. 2017 Jan 19;7:40802. [Content Brief]

[3]. Huang Y, et al. Effects of 4-DAMP on allergic rhinitis in guinea pigs and its potential mechanism. Ann Transl Med. 2022 Jul;10(14):774. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2156 mL	11.0781 mL	22.1562 mL	55.3906 mL
	5 mM	0.4431 mL	2.2156 mL	4.4312 mL	11.0781 mL
	10 mM	0.2216 mL	1.1078 mL	2.2156 mL	5.5391 mL
	15 mM	0.1477 mL	0.7385 mL	1.4771 mL	3.6927 mL
	20 mM	0.1108 mL	0.5539 mL	1.1078 mL	2.7695 mL
	25 mM	0.0886 mL	0.4431 mL	0.8862 mL	2.2156 mL
	30 mM	0.0739 mL	0.3693 mL	0.7385 mL	1.8464 mL
	40 mM	0.0554 mL	0.2770 mL	0.5539 mL	1.3848 mL
	50 mM	0.0443 mL	0.2216 mL	0.4431 mL	1.1078 mL
	60 mM	0.0369 mL	0.1846 mL	0.3693 mL	0.9232 mL
	80 mM	0.0277 mL	0.1385 mL	0.2770 mL	0.6924 mL
	100 mM	0.0222 mL	0.1108 mL	0.2216 mL	0.5539 mL

4-DAMP Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-DAMP1952-15-44-DAMP methiodidemAChRApoptosisMMPEGFRInterleukin RelatedMuscarinic acetylcholine receptorMatrix metalloproteinasesEpidermal growth factor receptorErbB-1HER1ILInhibitorinhibitorinhibit

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4-DAMP (Synonyms: 4-DAMP methiodide)

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おすすめ

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mAChR アイソフォーム固有の製品をすべて表示:

MMP アイソフォーム固有の製品をすべて表示:

EGFR アイソフォーム固有の製品をすべて表示:

Interleukin Related アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

4-DAMP Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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4-DAMP (Synonyms: 4-DAMP methiodide)

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4-DAMP 関連抗体

顧客検証

おすすめ

•関連小分子:

•関連タンパク質:

mAChR アイソフォーム固有の製品をすべて表示:

MMP アイソフォーム固有の製品をすべて表示:

EGFR アイソフォーム固有の製品をすべて表示:

Interleukin Related アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

4-DAMP 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

4-DAMP Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report