ベラパミル塩酸塩 (Synonyms: Verapamil (hydrochloride); (±)-Verapamil (hydrochloride); CP-16533-1 (hydrochloride))

Name: Verapamil hydrochloride
Brand: MedChemExpress
SKU: HY-A0064
Rating: 5.0 (86 reviews)

製品番号: HY-A0064 純度: 99.98%: Data Sheet SDS COA 取扱説明書
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Verapamil hydrochloride ((±)-Verapamil hydrochloride) is a calcium channel blocker and a potent and orally active first-generation P-glycoprotein (P-gp) inhibitor. Verapamil hydrochloride also inhibits CYP3A4. Verapamil hydrochloride has the potential for high blood pressure, heart arrhythmias and angina research.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 152-11-4

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solid
500 mg	$50	在庫あり	Estimated Time of Arrival: December 31
1 g	$60	在庫あり	Estimated Time of Arrival: December 31
5 g	$108	在庫あり	Estimated Time of Arrival: December 31
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カスタマーレビュー

Based on 86 publication(s) in Google Scholar

Other Forms of Verapamil hydrochloride:

顧客検証

Cell Proliferation/Viability Assay

RT-PCR

: Verapamil hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 May 5.; Cytosolic Ca²⁺ fluorescence images of BMSCs before and after inhibition of L-type calcium channels with Verapamil (10 μmol/L).

: Verapamil hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 May 15.; Verapamil (20 μM; 5 days). Western blotting of ERK and P-ERK levels in NSCs of the Control, Nanoelectrode+MF with Verapamil, and Nanoelectrode+MF groups.

: Verapamil hydrochloride purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Jun 19:S0891-5849(24)00531-8. [Abstract]; HT1080 cells were pretreated with Verapamil (20 μM) or MK-571 sodium for 2 h and then treated with DMSO or RU486 together with concentration gradient of RSL3 for 24 h. Cell viability was measured by CCK-8.

: Verapamil hydrochloride purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2023 May;48(5):1424-1437. [Abstract]; LPS-stimulated proinflammatory cytokine (IL-1β, IL-6, and TNF-α) expression and extracellular secretion in primary astrocytes are suppressed following administration of the P-gp inhibitor Verapamil (Ver; 70 μM).

: Verapamil hydrochloride purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2021 Apr 21;6(11):4073-4082. [Abstract]; Intracellular calcium fluorescence staining of MC3T3-E1 cells on day 1 in a blank and samples inhibited by Verapamil (10 μmol/L, 24 h) or SKF-96365 (10 μmol/L, 24 h).

: Verapamil hydrochloride purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2021 Oct;178(20):4155-4175. [Abstract]; Verapamil (Ver; 100 µM; 24 h). Effect of NaHS on neuronal viability against OGD/R injury in vitro. Cell viability was measured by CCK-8 at 24 h post-reoxygenation.

Calcium Channel アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

Cytochrome P450 アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

生物活性
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製品説明

IC₅₀ & Target

CYP3

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	0.2 3 Compound: Verapamil hydrochloride	Inhibition of human ERG expressed in CHO cells assessed as reduction in peak channel current by IonWork patch-clamp electrophysiology method Inhibition of human ERG expressed in CHO cells assessed as reduction in peak channel current by IonWork patch-clamp electrophysiology method	[PMID: 30562027]
CHO	IC₅₀	0.2 3 Compound: Verapamil hydrochloride	Inhibition of human ERG expressed in CHO cells assessed as reduction in peak channel current by IonWork patch-clamp electrophysiology method Inhibition of human ERG expressed in CHO cells assessed as reduction in peak channel current by IonWork patch-clamp electrophysiology method	[PMID: 30562027]
CHO	IC₅₀	0.2 3 Compound: Verapamil hydrochloride	Inhibition of human ERG expressed in CHO cells assessed as reduction in peak channel current by IonWork patch-clamp electrophysiology method Inhibition of human ERG expressed in CHO cells assessed as reduction in peak channel current by IonWork patch-clamp electrophysiology method	[PMID: 30562027]
K562	IC₅₀	100 3 Compound: VRP	Cytotoxicity against cell line K562 determined by MTT test Cytotoxicity against cell line K562 determined by MTT test	[PMID: 12431060]
K562	IC₅₀	100 3 Compound: VRP	Cytotoxicity against cell line K562 determined by MTT test Cytotoxicity against cell line K562 determined by MTT test	[PMID: 12431060]
K562	IC₅₀	100 3 Compound: VRP	Cytotoxicity against cell line K562i/S9 and pgp determined by MTT test Cytotoxicity against cell line K562i/S9 and pgp determined by MTT test	[PMID: 12431060]
Platelet	IC₅₀	577.5 3 Compound: Verapamil hydrochloride	Antimigraine activity in bovine citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting Antimigraine activity in bovine citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting	[PMID: 1431933]
Platelet	IC₅₀	770.2 3 Compound: Verapamil hydrochloride	Antimigraine activity in human citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting Antimigraine activity in human citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting	[PMID: 1431933]
Platelet	IC₅₀	577.5 3 Compound: Verapamil hydrochloride	Antimigraine activity in bovine citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting Antimigraine activity in bovine citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting	[PMID: 1431933]
Platelet	IC₅₀	770.2 3 Compound: Verapamil hydrochloride	Antimigraine activity in human citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting Antimigraine activity in human citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting	[PMID: 1431933]
K562	IC₅₀	100 3 Compound: VRP	Cytotoxicity against cell line K562 determined by MTT test Cytotoxicity against cell line K562 determined by MTT test	[PMID: 12431060]
K562	IC₅₀	100 3 Compound: VRP	Cytotoxicity against cell line K562i/S9 and pgp determined by MTT test Cytotoxicity against cell line K562i/S9 and pgp determined by MTT test	[PMID: 12431060]
Platelet	IC₅₀	577.5 3 Compound: Verapamil hydrochloride	Antimigraine activity in bovine citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting Antimigraine activity in bovine citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting	[PMID: 1431933]
Platelet	IC₅₀	770.2 3 Compound: Verapamil hydrochloride	Antimigraine activity in human citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting Antimigraine activity in human citreated platelet assessed as inhibition of [14C]serotonin release after 6 mins by scintillation counting	[PMID: 1431933]

体外実験

The EverFluor FL Verapamil (EFV) uptake by TR-iBRB2 cells is inhibited by cationic drugs, and inhibits by verapamil in a concentration-dependent manner with an IC₅₀ of 98.0 μM^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Given orally Verapamil is useful for the prophylaxis of atrioventricular reentry tachycardia, and also in modulating the atrioventricular nodal response in atrial fibrillation^[2].
Verapamil is injected i.v. into a femoral vein prior to ischemia. Verapamil (1 mg/kg) significantly decreases the incidence of ventricular arrhythmias including premature ventricular contractions (PVC), ventricular tachycardia (VT) and ventricular fibrillation (VF) for 45-min coronary artery occlusion. Total arrhythmia scores are significantly increased when the heart is subjected to ischemia. Verapamil (1 mg/kg) significantly prevents the enhancement of total arrhythmia scores induced by ischemia^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

491.06

分子式

C₂₇H₃₉ClN₂O₄

CAS 番号

152-11-4

Appearance

Solid

Color

White to off-white

SMILES

[H]Cl.COC1=CC=C(C(C#N)(C(C)C)CCCN(CCC2=CC=C(OC)C(OC)=C2)C)C=C1OC

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 58.33 mg/mL (118.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (101.82 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0364 mL	10.1821 mL	20.3641 mL
5 mM	0.4073 mL	2.0364 mL	4.0728 mL
10 mM	0.2036 mL	1.0182 mL	2.0364 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.18 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (10.18 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (10.18 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Saline
Solubility: ≥ 5 mg/mL (10.18 mM); Clear solution

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 25 mg/mL (50.91 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.98%

Select Batch:

データシート (279 KB) SDS (624 KB)

COA (249 KB) LCMS (106 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Gowarty JL, et al. Verapamil as a culprit of palbociclib toxicity. J Oncol Pharm Pract. 2019 Apr;25(3):743-746. [Content Brief]

[2]. Krikler DM. Verapamil in arrhythmia. Br J Clin Pharmacol. 1986;21 Suppl 2:183S-189S. [Content Brief]

[3]. Zhou P, et al. Anti-arrhythmic effect of Verapamil is accompanied by preservation of cx43 protein in rat heart. PLoS One. 2013 Aug 12;8(8):e71567. [Content Brief]

[4]. Rehnqvist N,et al. Effects of metoprolol vs verapamil in patients with stable angina pectoris. The Angina Prognosis Study in Stockholm (APSIS). Eur Heart J. 1996 Jan;17(1):76-81. [Content Brief]

[5]. Kubo Y, et al. Blood-to-Retina Transport of Fluorescence-Labeled Verapamil at the Blood-Retinal Barrier. Pharm Res. 2018 Mar 12;35(5):93. [Content Brief]

細胞実験 ^[1]	Cells (1×10⁵) are treated with 10 nM Bortezomib and/or 70 µM Verapamil for 16 hours and incubated for another 4 hours with Alamar-Blue. Activity of the mitochondrial dehydrogenase results in conversion of the coloring, which is followed by measurement of the absorption using a spectrophotometer^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[3]	Rats^[3] Adult male Sprague-Dawley (SD) rats (250−350 g) are used. Verapamil (1 mg/kg) is injected i.v. into a femoral vein 10 min prior to ischemia. A sham group undergoes the same surgical procedures, except the suture underneath the LAD is left untied. In another series of experiment, arrhythmia is induced by Bay K8644, an L-type calcium channel agonist, at a dose of 0.1 mg/kg given i.v. into the FV. Verapamil (1 mg/kg) is administered 10 min prior to Bay K8644. All injections are performed within 30 sec. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Gowarty JL, et al. Verapamil as a culprit of palbociclib toxicity. J Oncol Pharm Pract. 2019 Apr;25(3):743-746. [Content Brief] [2]. Krikler DM. Verapamil in arrhythmia. Br J Clin Pharmacol. 1986;21 Suppl 2:183S-189S. [Content Brief] [3]. Zhou P, et al. Anti-arrhythmic effect of Verapamil is accompanied by preservation of cx43 protein in rat heart. PLoS One. 2013 Aug 12;8(8):e71567. [Content Brief] [4]. Rehnqvist N,et al. Effects of metoprolol vs verapamil in patients with stable angina pectoris. The Angina Prognosis Study in Stockholm (APSIS). Eur Heart J. 1996 Jan;17(1):76-81. [Content Brief] [5]. Kubo Y, et al. Blood-to-Retina Transport of Fluorescence-Labeled Verapamil at the Blood-Retinal Barrier. Pharm Res. 2018 Mar 12;35(5):93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0364 mL	10.1821 mL	20.3641 mL	50.9103 mL
	5 mM	0.4073 mL	2.0364 mL	4.0728 mL	10.1821 mL
	10 mM	0.2036 mL	1.0182 mL	2.0364 mL	5.0910 mL
	15 mM	0.1358 mL	0.6788 mL	1.3576 mL	3.3940 mL
	20 mM	0.1018 mL	0.5091 mL	1.0182 mL	2.5455 mL
	25 mM	0.0815 mL	0.4073 mL	0.8146 mL	2.0364 mL
	30 mM	0.0679 mL	0.3394 mL	0.6788 mL	1.6970 mL
	40 mM	0.0509 mL	0.2546 mL	0.5091 mL	1.2728 mL
	50 mM	0.0407 mL	0.2036 mL	0.4073 mL	1.0182 mL
	60 mM	0.0339 mL	0.1697 mL	0.3394 mL	0.8485 mL
	80 mM	0.0255 mL	0.1273 mL	0.2546 mL	0.6364 mL
	100 mM	0.0204 mL	0.1018 mL	0.2036 mL	0.5091 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Verapamil hydrochloride Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ベラパミル塩酸塩 (Synonyms: Verapamil (hydrochloride); (±)-Verapamil (hydrochloride); CP-16533-1 (hydrochloride))

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Other Forms of Verapamil hydrochloride:

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純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Verapamil hydrochloride Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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ベラパミル塩酸塩 (Synonyms: Verapamil (hydrochloride); (±)-Verapamil (hydrochloride); CP-16533-1 (hydrochloride))

カスタマーレビュー

Other Forms of Verapamil hydrochloride:

ベラパミル塩酸塩 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Calcium Channel アイソフォーム固有の製品をすべて表示:

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生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

ベラパミル塩酸塩 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Verapamil hydrochloride Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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ベラパミル塩酸塩関連抗体

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