2. Epigenetics TGF-beta/Smad Stem Cell/Wnt PI3K/Akt/mTOR
  3. PKC GSK-3
  4. Bisindolylmaleimide I hydrochloride

Bisindolylmaleimide I hydrochloride  (Synonyms: GF109203X hydrochloride; Go 6850 hydrochloride)

Cat. No.: HY-13867A Purity: 98.44%
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Bisindolylmaleimide I (GF109203X) hydrochloride is a cell-permeable and reversible PKC inhibitor (IC50 of 20 nM, 17 nM, 16 nM, and 20 nM for PKCα, PKCβI, PKCβII, and PKCγ. Bisindolylmaleimide I hydrochloride is also a GSK-3 inhibitor.

For research use only. We do not sell to patients.

CAS No. : 176504-36-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
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10 mg In-stock
25 mg In-stock
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Customer Review

Based on 40 publication(s) in Google Scholar

Other Forms of Bisindolylmaleimide I hydrochloride:

Bisindolylmaleimide I hydrochloride Related Antibodies

Top Publications Citing Use of Products

40 Publications Citing Use of MCE Bisindolylmaleimide I hydrochloride

WB
Flow Cytometry
Cell Proliferation/Viability Assay
ELISA
Cell Imaging/Staining

    Bisindolylmaleimide I hydrochloride purchased from MedChemExpress. Usage Cited in: Neural Regen Res. 2025 Jul 1;20(7):2038-2052.  [Abstract]

    Cell viability was measured by cell counting kit-8 in MN9D cells with/without MPP+ 0.5 mM and Bisindolylmaleimide I (BIM) 5 µM treatment for 24 hours.

    Bisindolylmaleimide I hydrochloride purchased from MedChemExpress. Usage Cited in: Neural Regen Res. 2025 Jul 1;20(7):2038-2052.  [Abstract]

    C11-BODIPY fluorescence analysis suggested that MPP+ increased intracellular lipid peroxidation in MN9D cells, and that this effect was reversed by Bisindolylmaleimide I (BIM) (5 μM) treatment.

    Bisindolylmaleimide I hydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 11;11(11):5279-5295.  [Abstract]

    SH-SY5Y cells were simultaneously treated with C004019 (1 or 10 µM) and wortmainnin (WO, 1 µM) and Bisindolylmaleimide I (GF-109203X) (GFX, 1 µM) for 24 h to induce tau hyperphosphorylation, and then tau was measured by Western blotting.

    Bisindolylmaleimide I hydrochloride purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2022 Feb;15(2):314-326.  [Abstract]

    Lung ILC2s were treated with vehicle or UCB (50 μM) and /or Honokiol (5 μM) and /or Bisindolylmaleimide I (BIM1) (5 μM) in the presence of IL-2, IL-7 and IL-33 for 48 h. Flow cytometric analysis of p-ERK1/2 levels was performed.

    Bisindolylmaleimide I hydrochloride purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2022 Feb;15(2):314-326.  [Abstract]

    The amounts of IL-5 and IL-13 in the supernatants were measured by ELISA.

    View All PKC Isoform Specific Products:

    View All Isoforms
    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

    View All GSK-3 Isoform Specific Products:

    View All Isoforms
    GSK-3 GSK-3α GSK-3β

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Bisindolylmaleimide I (GF109203X) hydrochloride is a cell-permeable and reversible PKC inhibitor (IC50 of 20 nM, 17 nM, 16 nM, and 20 nM for PKCα, PKCβI, PKCβII, and PKCγ. Bisindolylmaleimide I hydrochloride is also a GSK-3 inhibitor[1][2][3].

    IC50 & Target[1]

    Bovine brain PKC

    10 nM (IC50)

    PKC-βII

    16 nM (IC50)

    PKC-βI

    17 nM (IC50)

    PKCα

    20 nM (IC50)

    PKCγ

    20 nM (IC50)

    FDGFG

    65 μM (IC50)

    In Vitro

    Bisindolylmaleimide I hydrochloride (5 μM) inhibits α-thrombin-induced P47 phosphorylation[1].
    Bisindolylmaleimide I hydrochloride (0-1 μM) inhibits DNA synthesis in quiescent swiss 3T3 cells[1].
    Bisindolylmaleimide I hydrochloride (5 μM) reduces GSK-3 activity to 25.1±4.3% in adipocytes lysates[3].
    Bisindolylmaleimide I hydrochloride (10 μM, 24 h) inhibits exosome and microvesicle (EMV) release from PC3 cells[4].
    Bisindolylmaleimide I hydrochloride (10 μM, 24 h) enhances cytotoxicity of 5-fluorouracil (HY-90006)[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bisindolylmaleimide I hydrochloride Related Antibodies
    In Vivo

    Bisindolylmaleimide I hydrochloride (0.02 mg/kg, i.p.) reduced the inceased NLRP3, P-PKCɑ, and PKCɑ levels in mechanical ventilation (MV) group[5].
    Bisindolylmaleimide I hydrochloride (0-20 mg/kg, i.p.) reduces the mean frequency of Quinpirole-induced vomiting in shrews[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Quinpirole-treated shrews[2]
    Dosage: 0-20 mg/kg
    Administration: i.p.
    Result: Reduced the mean frequency of Quinpirole-induced vomiting.
    Blocked Quinpirole-mediated ERK1/2 phosphorylation in shrew brainstems.
    Molecular Weight

    448.94

    Formula

    C25H25ClN4O2
    CAS No.
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    [H]Cl.O=C(C(C1=CN(CCCN(C)C)C2=C1C=CC=C2)=C3C4=CNC5=C4C=CC=C5)NC3=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 62.5 mg/mL (139.22 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2275 mL 11.1373 mL 22.2747 mL
    5 mM 0.4455 mL 2.2275 mL 4.4549 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

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    Concentration (final)

    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (4.63 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2275 mL 11.1373 mL 22.2747 mL 55.6867 mL
    5 mM 0.4455 mL 2.2275 mL 4.4549 mL 11.1373 mL
    10 mM 0.2227 mL 1.1137 mL 2.2275 mL 5.5687 mL
    15 mM 0.1485 mL 0.7425 mL 1.4850 mL 3.7124 mL
    20 mM 0.1114 mL 0.5569 mL 1.1137 mL 2.7843 mL
    25 mM 0.0891 mL 0.4455 mL 0.8910 mL 2.2275 mL
    30 mM 0.0742 mL 0.3712 mL 0.7425 mL 1.8562 mL
    40 mM 0.0557 mL 0.2784 mL 0.5569 mL 1.3922 mL
    50 mM 0.0445 mL 0.2227 mL 0.4455 mL 1.1137 mL
    60 mM 0.0371 mL 0.1856 mL 0.3712 mL 0.9281 mL
    80 mM 0.0278 mL 0.1392 mL 0.2784 mL 0.6961 mL
    100 mM 0.0223 mL 0.1114 mL 0.2227 mL 0.5569 mL
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    Bisindolylmaleimide I hydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bisindolylmaleimide I176504-36-2GF109203XGo 6850Go6850Go-6850PKCGSK-3Protein kinase CGlycogen synthase kinase-3Glycogen synthase kinase 3Exosome3T3adipocyte5-fluorouracilP47shrewsInhibitorinhibitorinhibit

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