Lidocaine hydrochloride hydrate
Based on 23 publication(s) in Google Scholar
Lidocaine (Lignocaine) hydrochloride hydrate inhibits sodium channels involving complex voltage and using dependence. Lidocaine hydrochloride hydrate decreases growth, migration and invasion of gastric carcinoma cells via up-regulating miR-145 expression and further inactivation of MEK/ERK and NF-κB signaling pathways. Lidocaine hydrochloride hydrate is an amide derivative and has potential for the research of ventricular arrhythmia.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 6108-05-0
- Formula: C14H25ClN2O2
- Molecular Weight:288.81
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Lidocaine hydrochloride hydrate
More- Nat Methods. 2021 Jul;18(7):788-798. [Abstract]
- Nat Commun. 2026 Feb 10;17(1):2559. [Abstract]
- Sci Adv. 2026 Mar 6;12(10):eadw0891. [Abstract]
- Sci Adv. 2025 Dec 5;11(49):eadw7972. [Abstract]
- J Neuroinflammation. 2017 Nov 2;14(1):211. [Abstract]
- Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
- Transl Psychiatry. 2025 May 17;15(1):172. [Abstract]
- J Ethnopharmacol. 2025 May 12:347:119803. [Abstract]
- Biomater Adv. 2026 Jan:178:214452. [Abstract]
- Drug Des Devel Ther. 2024 Apr 9:18:1103-1114. [Abstract]
- Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]
- Int Immunopharmacol. 2023 Feb:115:109706. [Abstract]
- Mol Med Rep. 2022 May;25(5):150. [Abstract]
- PLoS Pathog. 2023 Feb 3;19(2):e1011126. [Abstract]
- Phytochemistry. 2026 Aug:248:114929. [Abstract]
- Reprod Toxicol. 2025 May:134:108895. [Abstract]
- Eur Spine J. 2022 Nov;31(11):2960-2971. [Abstract]
- J Phys D Appl Phys. 2019 Aug; 52(46).
- Neurogastroenterol Motil. 2023 Nov;35(11):e14677. [Abstract]
- PLoS One. 2025 Nov 4;20(11):e0335932. [Abstract]
- Dig Dis Sci. 2021 Dec;66(12):4384-4397. [Abstract]
- Eng Proc. 2026 Jan 14;110(1):7.
- University of Glasgow. 2023.
Customer Validation & Images
-
RT-PCR
-
Cell Proliferation/Viability Assay
-
Cell Imaging/Staining
-
Cell Imaging/Staining
-
Cell Imaging/Staining
All MEK Isoforms
More
Biological Activity
|
ERK |
NF-κB |
MEK |
Lidocaine (Lignocaine) (10 nM; 48 hours) decreases significantly cell proliferation[2].
Lidocaine (1-10 nM; 24-72 hours) inhibits cell viability and achieves the most suppressing effects at the concentration of 10?nM and treatment time 48?hours[2].
Lidocaine (10 nM; 48 hours) increases significantly the apoptotic cell rate[2].
Lidocaine (10 nM; 48 hours) down-regulates Cyclin D1 and up-regulates p21 expression significantly[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:The human gastric cancer cell line MKN45
-
Concentration:10 nM
-
Incubation Time:48 hours
-
Result:Decreased significantly cell proliferation.
-
Cell Line:The human gastric cancer cell line MKN45
-
Concentration:1, 5 and 10 nM
-
Incubation Time:24, 48, 72 hours
-
Result:Inhibited MKN45 cell viability.
-
Cell Line:The human gastric cancer cell line MKN45
-
Concentration:10 nM
-
Incubation Time:48 hours
-
Result:Increased significantly the apoptotic cell rate.
-
Cell Line:The human gastric cancer cell line MKN45
-
Concentration:10 nM
-
Incubation Time:48 hours
-
Result:Down-regulated Cyclin D1 and up-regulated p21 expression significantly.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 6108-05-0
-
Appearance Solid
-
Molecular Weight 288.81
-
Formula C14H25ClN2O2
-
Color White to off-white
-
SMILES
O=C(NC1=C(C)C=CC=C1C)CN(CC)CC.Cl.O
-
Synonyms
Lignocaine hydrochloride hydrate
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (23)
-
Journal Impact Factor
-
Most Recent
-
Nat Methods
2021 Jul;18(7):788-798. PMID: 34127857 -
Nat Commun
Molecular determinant of low-voltage dependence of human Nav1.7 inactivation revealed by efficacy-based Nav1.7 selective inhibitor. [Abstract]2026 Feb 10;17(1):2559. PMID: 41667447 -
Sci Adv
A worm-like nucleic acid nanostructure for gene delivery and endosomal escape via ClC3 ion exchanger. [Abstract]2026 Mar 6;12(10):eadw0891. PMID: 41790895 -
Sci Adv
EphB1-NR2B receptor signaling in glutamatergic neurons of the ventroposteromedial thalamic nucleus regulates emergence from anesthesia. [Abstract]2025 Dec 5;11(49):eadw7972. PMID: 41348875 -
J Neuroinflammation
Lidocaine alleviates morphine tolerance via AMPK-SOCS3-dependent neuroinflammation suppression in the spinal cord. [Abstract]2017 Nov 2;14(1):211. PMID: 29096659
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2017 Nov 2;14(1):211. [Abstract]
Immunoblot results demonstrate that Lidocaine inhibits Morphine-induced upregulation of phosphorylation of p38 MAPK, but not the p38 total protein.
-
Stem Cell Res Ther
Local anesthetics impair the growth and self-renewal of glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130 palmitoylation. [Abstract]2021 Feb 4;12(1):107. PMID: 33541421
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
RT-PCR analysis of ZDHHC15 mRNA levels in GSCs treated with different concentrations (5, 10, and 20 μM) of Prilocaine, Lidocaine, Procaine, and Ropivacaine. β-Actin was used as a loading control.
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
Local anesthetics Prilocaine, Lidocaine (5-20 μM, 24-48 h), Procain, and Ropivacaine impaired cell survival through inhibition of ZDHHC15 expression.
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
Representative images showing U251 GSCs maintained under neurosphere conditions for 7 days after transfection with ZDHHC15 shRNA or treatment with prilocaine, lidocaine, procaine, or ropivacaine (20 μM).
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
Representative images showing U251 GSCs maintained under neurosphere conditions for 7 days after transfection with ZDHHC15 shRNA or treatment with prilocaine, lidocaine, procaine, or ropivacaine (20 μM). GSC neurospheres of all categories were stained for stem and differentiated cell markers as indicated. Cells were stained with antibodies against nestin and SOX2 for neural stem cell markers, and antibody staining of GFAP and MAP 2 were used as markers of differentiated cells. DAPI (4′,6-diamidino-2-phenylindole) was used as a nuclear stain.
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
The expression of GP130 in U251 GSCs (monolayer culture) transfected with ZDHHC5 shRNA and treated with Prilocaine, Lidocaine, Procaine, and Ropivacaine (20 μM) for 48 h was analyzed by immunofluorescence staining. Scale bar, 100 μm.
Lidocaine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Feb 4;12(1):107. [Abstract]
GSCs were transfected with ZDHHC5 shRNA or treated with Prilocaine, Lidocaine, Procaine, and Ropivacaine (20 μM) and harvested after 48 h. Cellular fractionation was performed to separate cytosolic and membrane fractions.
-
Transl Psychiatry
Stellate ganglion block diminishes consolidation of conditioned fear memory in mice by inhibiting the locus coeruleus to the basolateral amygdala neural circuit. [Abstract]2025 May 17;15(1):172. PMID: 40382311 -
J Ethnopharmacol
Traditional Chinese Medicine YangxinDingji alleviates arrhythmias through inhibition of sodium and L-type calcium channels. [Abstract]2025 May 12:347:119803. PMID: 40239882 -
Biomater Adv
2026 Jan:178:214452. PMID: 40876091 -
Drug Des Devel Ther
Dexmedetomidine Prolongs Lidocaine Intravenous Regional Anesthesia in Rats by Blocking the Hyperpolarization-Activated Cation Current. [Abstract]2024 Apr 9:18:1103-1114. PMID: 38618283 -
Cell Rep Methods
RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]2023 Oct 23;3(10):100599. PMID: 37797618 -
Int Immunopharmacol
Lidocaine inhibits influenza a virus replication by up-regulating IFNα4 via TBK1-IRF7 and JNK-AP1 signaling pathways. [Abstract]2023 Feb:115:109706. PMID: 36638664 -
Mol Med Rep
Lidocaine attenuates hypoxia/reoxygenation‑induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway. [Abstract]2022 May;25(5):150. PMID: 35244190 -
PLoS Pathog
Picornavirus infection enhances aspartate by the SLC38A8 transporter to promote viral replication. [Abstract]2023 Feb 3;19(2):e1011126. PMID: 36735752 -
Phytochemistry
Discovery of GABA-containing cyclic peptides from the cold-seep-derived fungus Aspergillus unguis SCSIO 41322 for the treatment of inflammatory bowel disease. [Abstract]2026 Aug:248:114929. PMID: 41997396 -
Reprod Toxicol
2025 May:134:108895. PMID: 40097051 -
Eur Spine J
Effect of species, concentration and volume of local anesthetics on intervertebral disk degeneration in rats with discoblock. [Abstract]2022 Nov;31(11):2960-2971. PMID: 36152221 -
-
Neurogastroenterol Motil
Lidocaine ameliorates intestinal barrier dysfunction in irritable bowel syndrome by modulating corticotropin-releasing hormone receptor 2. [Abstract]2023 Nov;35(11):e14677. PMID: 37736684 -
PLoS One
The efficacy and safety of lignocaine-embedded dissolvable microneedle versus EMLA for topical analgesia in adults undergoing venepuncture: A single-centre, parallel-group, double-blind randomised clinical trial protocol in a tertiary care setting. [Abstract]2025 Nov 4;20(11):e0335932. PMID: 41187145 -
Dig Dis Sci
Lidocaine Inhibits Hepatocellular Carcinoma Development by Modulating circ_ITCH/miR-421/CPEB3 Axis. [Abstract]2021 Dec;66(12):4384-4397. PMID: 33433806 -
-
Solvent & Solubility
H2O : ≥ 100 mg/mL (346.25 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Saline
Solubility: ≥ 100 mg/mL (346.25 mM); Clear solution
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
-
Handling Instructions (2659 KB)
References
[1]. Cummins TR, et al. Setting up for the block: the mechanism underlying lidocaine's use-dependent inhibition of sodium channels. J Physiol. 2007 Jul 1;582(Pt 1):11. [Content Brief]
[2]. Sui H, et al. Lidocaine inhibits growth, migration and invasion of gastric carcinoma cells by up-regulation of miR-145. BMC Cancer. 2019 Mar 15;19(1):233. [Content Brief]
[3]. Li Z, et al. Evaluation of the antinociceptive effects of lidocaine and bupivacaine on the tail nerves of healthy rats. Basic Clin Pharmacol Toxicol. 2013 Jul;113(1):31-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.4625 mL | 17.3124 mL | 34.6248 mL | 86.5621 mL |
| 5 mM | 0.6925 mL | 3.4625 mL | 6.9250 mL | 17.3124 mL | |
| 10 mM | 0.3462 mL | 1.7312 mL | 3.4625 mL | 8.6562 mL | |
| 15 mM | 0.2308 mL | 1.1542 mL | 2.3083 mL | 5.7708 mL | |
| 20 mM | 0.1731 mL | 0.8656 mL | 1.7312 mL | 4.3281 mL | |
| 25 mM | 0.1385 mL | 0.6925 mL | 1.3850 mL | 3.4625 mL | |
| 30 mM | 0.1154 mL | 0.5771 mL | 1.1542 mL | 2.8854 mL | |
| 40 mM | 0.0866 mL | 0.4328 mL | 0.8656 mL | 2.1641 mL | |
| 50 mM | 0.0692 mL | 0.3462 mL | 0.6925 mL | 1.7312 mL | |
| 60 mM | 0.0577 mL | 0.2885 mL | 0.5771 mL | 1.4427 mL | |
| 80 mM | 0.0433 mL | 0.2164 mL | 0.4328 mL | 1.0820 mL | |
| 100 mM | 0.0346 mL | 0.1731 mL | 0.3462 mL | 0.8656 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Lidocaine hydrochloride hydrate Related Classifications
HY-B0185B Related Classifications
Powered by Bioz