Sodium acetate trihydrate, meets analytical specification of Ph. Eur. BP USP FCC E262, ≤0.00002% Al

Name: Sodium acetate trihydrate, meets analytical specification of Ph. Eur. BP USP FCC E262, ≤0.00002% Al
Brand: MedChemExpress
SKU: HY-Y1325H
Rating: 4.5 (1 reviews)

Cat. No.: HY-Y1325H Assay: 100.4%: Data Sheet
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Sodium acetate trihydrate is a carboxylic acid and short-chain fatty acid (SCFAs). Sodium acetate trihydrate activates AMPK, increases ROS, cleaved caspase 9, PPARα, downregulates SREBP-1c, ChREBP expression. Sodium acetate trihydrate exhibits antifungal activity against Saccharomyces cerevisiae W303-1A. Sodium acetate trihydrate regulates energy metabolism. Sodium acetate trihydrate has anticancer activity against gastric cancer. Sodium acetate trihydrate induces writhing reaction and ulcerative colitis. Sodium acetate trihydrate can be used in the researches for gastric cancer, ulcerative colitis, hepatic steatosis, and pain.

For research use only. We do not sell to patients.

Sodium acetate trihydrate, meets analytical specification of Ph. Eur. BP USP FCC E262, ≤0.00002% Al Chemical Structure

CAS No. : 6131-90-4

Size	Price	Stock	Quantity
100 g		In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Sodium acetate trihydrate, meets analytical specification of Ph. Eur. BP USP FCC E262, ≤0.00002% Al:

Sodium acetate trihydrate, United States Pharmacopeia (USP) Reference Standard Get quote

1 Publications Citing Use of MCE Sodium acetate trihydrate, meets analytical specification of Ph. Eur. BP USP FCC E262, ≤0.00002% Al

•Cell Death Dis. 2025 Dec 18. [Abstract]

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1 BRSK1 BRSK2 HUNK AMPKα

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]^[3]^[4]^[5]^[6]^[7]^[8]^[9]

Microbial Metabolite

Human Endogenous Metabolite

PPARα

Caspase 9

In Vitro

Sodium acetate trihydrate (Acetic acid; 2-5 µM; 24 h) induces cancer cell-selective death in gastric cancer cells (RGK1) via oxidative stress, while having minor effects on normal gastric mucosal cells (RGM1)^[3].
Sodium acetate trihydrate (Acetic acid; 20-200 mM; 200 min) induces programmed cell death in Saccharomyces cerevisiae W303-1A^[4].
Sodium acetate trihydrate (Acetic acid; 5 mM; 15 d) suppresses the increase in disaccharidase activity (sucrase, maltase, trehalase, lactase) in Caco-2 cells without affecting cell growth or glucose transport^[5].
Sodium acetate trihydrate (Acetic acid; 1.8-7.2 mM; 3 h) activates the AMPKα signaling pathway by consuming ATP to increase the AMP/ATP ratio in bovine hepatocytes, upregulating lipid oxidation genes, downregulating lipogenic genes, and reducing intracellular triglyceride content^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Rat gastric mucosal cells (RGM1), cancerous gastric mucosal cells (RGK1)
Concentration:	0, 1, 2, 5, 10, 20 µM
Incubation Time:	24 h
Result:	Increased monocarboxylic transporter 1 (MCT1) and cleaved caspase 9 in RGK1 cells. Showed minor effects in RGM1 cells.

Western Blot Analysis^[6]

Cell Line:	Bovine hepatocytes
Concentration:	1.8 mM, 3.6 mM, 7.2 mM
Incubation Time:	3 h
Result:	Increased AMPKα phosphorylation and activity, upregulated PPARα expression, downregulated SREBP-1c and ChREBP expression.

In Vivo

Sodium acetate trihydrate (Acetic acid; 0.56%; i.p.) significantly induces writhing responses in C57BL/6J mice^[7].
Sodium acetate trihydrate (Acetic acid; 5% v/v; rectal instillation) induces ulcerative colitis in mice, causing weight loss, diarrhea, colonic inflammation, and tissue damage^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

136.08

Formula

C₂H₉NaO₅

CAS No.

6131-90-4

Appearance

Solid

Color

White to off-white

SMILES

[CH3COONa.3H2O]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 175 mg/mL (1286.01 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.3486 mL	36.7431 mL	73.4862 mL
5 mM	1.4697 mL	7.3486 mL	14.6972 mL
10 mM	0.7349 mL	3.6743 mL	7.3486 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 100.4%

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Data Sheet (279 KB) SDS (252 KB)

COA (249 KB)

Handling Instructions (2659 KB)

References

[1]. Syka JE, et al. Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry. Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9528-33. [Content Brief]

[2]. Barbier Jr J, et al. Total oxidation of acetic acid in aqueous solutions over noble metal catalysts. Journal of Catalysis, 1998, 177(2): 378-385.

[3]. Terasaki M, et al. Acetic acid is an oxidative stressor in gastric cancer cells. J Clin Biochem Nutr. 2018 Jul;63(1):36-41. [Content Brief]

[4]. Ludovico P, et al. Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid. Microbiology (Reading). 2001 Sep;147(Pt 9):2409-2415. [Content Brief]

[5]. Ogawa N, et al. Acetic acid suppresses the increase in disaccharidase activity that occurs during culture of caco-2 cells. J Nutr. 2000 Mar;130(3):507-13. [Content Brief]

[6]. Li X, et al. Acetic acid activates the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes. PLoS One. 2013 Jul 4;8(7):e67880. [Content Brief]

[7]. Stevenson GW, et al. Targeting pain-suppressed behaviors in preclinical assays of pain and analgesia: effects of morphine on acetic acid-suppressed feeding in C57BL/6J mice. J Pain. 2006 Jun;7(6):408-16. [Content Brief]

[8]. Niu X, et al. Protective effect of sanguinarine against acetic acid-induced ulcerative colitis in mice. Toxicol Appl Pharmacol. 2013 Mar 15;267(3):256-65. [Content Brief]

[9]. Granado-Serrano AB, et al. Faecal bacterial and short-chain fatty acids signature in hypercholesterolemia. Sci Rep. 2019 Feb 11;9(1):1772. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	7.3486 mL	36.7431 mL	73.4862 mL	183.7155 mL
	5 mM	1.4697 mL	7.3486 mL	14.6972 mL	36.7431 mL
	10 mM	0.7349 mL	3.6743 mL	7.3486 mL	18.3715 mL
	15 mM	0.4899 mL	2.4495 mL	4.8991 mL	12.2477 mL
	20 mM	0.3674 mL	1.8372 mL	3.6743 mL	9.1858 mL
	25 mM	0.2939 mL	1.4697 mL	2.9394 mL	7.3486 mL
	30 mM	0.2450 mL	1.2248 mL	2.4495 mL	6.1238 mL
	40 mM	0.1837 mL	0.9186 mL	1.8372 mL	4.5929 mL
	50 mM	0.1470 mL	0.7349 mL	1.4697 mL	3.6743 mL
	60 mM	0.1225 mL	0.6124 mL	1.2248 mL	3.0619 mL
	80 mM	0.0919 mL	0.4593 mL	0.9186 mL	2.2964 mL
	100 mM	0.0735 mL	0.3674 mL	0.7349 mL	1.8372 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.