2. Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. Endogenous Metabolite Apoptosis Reactive Oxygen Species (ROS) Caspase
  4. Citicoline sodium

Citicoline sodium  (Synonyms: Cytidine diphosphate-choline sodium; CDP-Choline sodium; Cytidine 5'-diphosphocholine sodium)

Cat. No.: HY-B0739A Purity: 99.77%
COA
SDS
Handling Instructions Technical Support

Citicoline sodium is an endogenous intermediate in the synthesis of phosphatidylcholine which is a component of cell membranes. Citicoline sodium inhibits reactive oxygen species (ROS) and apoptosis. Citicoline sodium can be used for neurological disease and hearing loss study.

For research use only. We do not sell to patients.

CAS No. : 33818-15-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
100 mg In-stock
500 mg In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Citicoline sodium:

Citicoline sodium Related Antibodies

Top Publications Citing Use of Products

    Citicoline sodium purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 Apr;26(4):542-554.  [Abstract]

    In vivo EEG and ex vivo MEA recordings of KA epileptic mice treated with CDP-choline (Citicoline: 100 mg/kg) or vehicle control.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    View All Caspase Isoform Specific Products:

    View All Isoforms
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Citicoline sodium is an endogenous intermediate in the synthesis of phosphatidylcholine which is a component of cell membranes. Citicoline sodium inhibits reactive oxygen species (ROS) and apoptosis. Citicoline sodium can be used for neurological disease and hearing loss study[1][2][3].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Caspase-8

     

    Caspase 3

     

    Caspase-9

     

    In Vitro

    Citicoline (1-100 μM, 24 h or 24 h before glutamate treatment) sodium increases rats retinal cells viability after glutamate-induced excitotoxicity, and has synergistic effects with Tramiprosate (Homotaurine) (HY-14602)[1].
    Citicoline (100 μM, 24 h before glutamate treatment and 30 min before HG treatment) sodium decreases proapoptotic effects associated with neuroretinal degeneration such as glutamate-induced excitotoxicity and high glucose (HG)-induced neurotoxicity, and has synergistic effects with Tramiprosate in rats retinal cells[1].
    Citicoline (100 μM, 36-48 h) sodium reduces apoptosis in mice cochlear hair cells (HCs) and HEI-OC-1 cells after Neomycin (HY-150520) exposure, decreases the mRNA level of pro-apoptotic factor and increases anti-apoptotic factor, suppresses proapoptotic protein expression[2][3].
    Citicoline (100 μM, 36-48 h) sodium attenuates oxidative stress in mice cochlear HCs and HEI-OC-1 cells after Neomycin injury[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Citicoline sodium Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Rat retinal cells
    Concentration: 1, 10, 100 μM
    Incubation Time: 24 h or 24 h before glutamate treatment (100 μM)
    Result: Did not affect viability of primary retinal cultures.
    Significantly increased cell viability after glutamate-induced excitotoxicity, and had synergistic effects with homotaurine.

    Apoptosis Analysis[1]

    Cell Line: Rat retinal cells
    Concentration: 100 μM
    Incubation Time: 24 h before glutamate treatment and 30 min before HG treatment
    Result: Reduced apoptosis induced by administration of glutamate and HG, and had synergistic effects with homotaurine.

    Apoptosis Analysis[3]

    Cell Line: HEI-OC-1, mice cochlear tissue
    Concentration: 10 μM
    Incubation Time: 36 or 48 h (pretreatment for 12 h and then treated with 10 mM neomycin together for 12 and 24 h in cochlear tissue and HEI-OC-1, respectively, then allowed to recover in culture medium for another 12
    Result: Showed significantly lower numbers of cleaved caspase 3-positive cells and TUNEL-positive cells than the neomycin-only group.
    Significantly reduced the percentage of apoptosis.
    The protein levels of cleaved caspase 3 were decreased compared with the Neomycin-only group.
    Decreased the pro-apoptotic factor Bax, Casp3, Casp8, and Casp9, and increased anti-apoptotic factor Bcl-2 by qPT-PCR analysis.
    In Vivo

    Citicoline (500-1000 mg/kg; i.p.; 1 h before test) sodium produces an anticonvulsant effect in pentylenetetrazole induced convulsion in male C57Bl/6 mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57Bl/6 mice (23-27 g) (The solution of convulsant pentylenetetrazole (1%) was infused intravenously at a flow rate of 0.01 ml/sec.)[2]
    Dosage: 500, 1000 mg/kg
    Administration: Intraperitoneal injection (i.p.); 1 h before test
    Result: Increased the threshold of clonic seizures and tonic phase of seizures with lethal outcome in a dose of 500 mg/kg.
    The anticonvulsant effect was most pronounced in a dose of 1000 mg/kg.
    Clinical Trial
    Molecular Weight

    510.31

    Formula

    C14H25N4NaO11P2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(C=CN1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OCC[N+](C)(C)C)([O-])=O)(O[Na])=O)O2)=NC1=O
    Structure Classification
    Initial Source

    Widespread
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (195.96 mM)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9596 mL 9.7980 mL 19.5959 mL
    5 mM 0.3919 mL 1.9596 mL 3.9192 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (195.96 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9596 mL 9.7980 mL 19.5959 mL 48.9898 mL
    5 mM 0.3919 mL 1.9596 mL 3.9192 mL 9.7980 mL
    10 mM 0.1960 mL 0.9798 mL 1.9596 mL 4.8990 mL
    15 mM 0.1306 mL 0.6532 mL 1.3064 mL 3.2660 mL
    20 mM 0.0980 mL 0.4899 mL 0.9798 mL 2.4495 mL
    25 mM 0.0784 mL 0.3919 mL 0.7838 mL 1.9596 mL
    30 mM 0.0653 mL 0.3266 mL 0.6532 mL 1.6330 mL
    40 mM 0.0490 mL 0.2449 mL 0.4899 mL 1.2247 mL
    50 mM 0.0392 mL 0.1960 mL 0.3919 mL 0.9798 mL
    60 mM 0.0327 mL 0.1633 mL 0.3266 mL 0.8165 mL
    80 mM 0.0245 mL 0.1225 mL 0.2449 mL 0.6124 mL
    100 mM 0.0196 mL 0.0980 mL 0.1960 mL 0.4899 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Citicoline sodium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Citicoline33818-15-4Cytidine diphosphate-cholineCDP-CholineCytidine 5'-diphosphocholineEndogenous MetaboliteApoptosisReactive Oxygen Species (ROS)Caspasereactive oxygen speciesneurologicalhearing lossInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Citicoline sodium
    Cat. No.:
    HY-B0739A
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.