2. Anti-infection Apoptosis Metabolic Enzyme/Protease Membrane Transporter/Ion Channel
  3. HIV Apoptosis Endogenous Metabolite Cytochrome P450 P-glycoprotein
  4. Limonin

リモニン  (Synonyms: Limonin; Limonoic acid 3,19:16,17 dilactone)

製品番号: HY-17411 純度: 99.38%
COA 取扱説明書 Technical Support

Limonin inhibits HIV-1 with an EC50 of 60.0 μM. Limonin induces human colon adenocarcinoma cells apoptosis with an IC50 of 54.74 μM. Limonin has antiviral and antitumor activities.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 1180-71-8

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
50 mg $50 在庫あり
100 mg $77 在庫あり
250 mg $125 在庫あり
500 mg $175 在庫あり
1 g $245 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 13 publication(s) in Google Scholar

Other Forms of Limonin:

リモニン 関連抗体

Top Publications Citing Use of Products

    Limonin purchased from MedChemExpress. Usage Cited in: Immun Inflamm Dis. 2023 Feb;11(2):e787.  [Abstract]

    Limonin (40 mg/kg; p.o.; single daily for 4 days) significantly increases the expression of ZO-1, Occludin, and claudin-1 in small intestinal tissues of rats.

    HIV アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    HIV HIV-1 HIV-2

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    Cytochrome P450 アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Limonin inhibits HIV-1 with an EC50 of 60.0 μM. Limonin induces human colon adenocarcinoma cells apoptosis with an IC50 of 54.74 μM. Limonin has antiviral and antitumor activities[1][2][4].

    IC50 & Target

    HIV-1

     

    Cellular Effect
    Cell Line Type Value Description References
    H9 IC50
    2.11 ng/mL
    Compound: Limonin
    Inhibition of HIV1 3B reverse transcriptase activity infected in human H9 cells assessed as level of p24 antigen
    Inhibition of HIV1 3B reverse transcriptase activity infected in human H9 cells assessed as level of p24 antigen
    		[PMID: 21334901]
    MCF7 IC50
    4.3 μM
    Compound: 11; LMN
    Antiproliferative activity against human MCF7 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
    Antiproliferative activity against human MCF7 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
    		[PMID: 33289552]
    MDA-MB-435 IC50
    26 μM
    Compound: 11; LMN
    Antiproliferative activity against human MDA-MB-435 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
    Antiproliferative activity against human MDA-MB-435 cells incubated for 48 hrs by [3H]-thymidine incorporation assay
    		[PMID: 33289552]
    MOLT-3 CC50
    201.56 μg/mL
    Compound: Limonin
    Cytotoxicity against human MOLT3 cells after 24 hrs by MTS assay
    Cytotoxicity against human MOLT3 cells after 24 hrs by MTS assay
    		[PMID: 21334901]
    MT2 IC50
    0.31 ng/mL
    Compound: Limonin
    Inhibition of Human T-cell leukemia/lymphoma virus type 1 reverse transcriptase activity infected in human MT2 cells assessed as level of p19 antigen
    Inhibition of Human T-cell leukemia/lymphoma virus type 1 reverse transcriptase activity infected in human MT2 cells assessed as level of p19 antigen
    		[PMID: 21334901]
    Neutrophil IC50
    38.2 μM
    Compound: 14
    Immunomodulatory activity in polymorphoneutrophils assessed as inhibition of luminol-induced oxidative burst by chemiluminescence assay
    Immunomodulatory activity in polymorphoneutrophils assessed as inhibition of luminol-induced oxidative burst by chemiluminescence assay
    		[PMID: 18950230]
    PBMC CC50
    451.91 μg/mL
    Compound: Limonin
    Cytotoxicity against PHA-stimutated human PBMC after 24 hrs by MTS assay
    Cytotoxicity against PHA-stimutated human PBMC after 24 hrs by MTS assay
    		[PMID: 21334901]
    体外実験

    Limonin (100 μM) exhibits cytotoxic effect and inhibits SW480 cells proliferation [2].
    Limonin (40 μM) inhibits HIV-1 protease in PBMC of HIV-1 infected patients[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    リモニン 関連抗体

    Cell Viability Assay[2]

    Cell Line: SW480 cells
    Concentration: 25, 50, 75, 100 μM
    Incubation Time: 4, 6 days
    Result: Inhibited cell proliferation by 89% at 4 days with 100 μM.
    Inhibited cell proliferation by 75% at 6 days with 100 μM.
    分子量

    470.51

    分子式

    C26H30O8
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1[C@@H]2[C@@]3(O2)[C@](CC[C@@]([C@@]4([C@@](CC(OC4)=O)([H])OC5(C)C)[C@@]5([H])CC6=O)([H])[C@]36C)(C)[C@H](C7=COC=C7)O1
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 41.67 mg/mL (88.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1254 mL 10.6268 mL 21.2535 mL
    5 mM 0.4251 mL 2.1254 mL 4.2507 mL
    10 mM 0.2125 mL 1.0627 mL 2.1254 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (4.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.38%

    COA (267 KB) HNMR (267 KB) LCMS (87 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1254 mL 10.6268 mL 21.2535 mL 53.1338 mL
    5 mM 0.4251 mL 2.1254 mL 4.2507 mL 10.6268 mL
    10 mM 0.2125 mL 1.0627 mL 2.1254 mL 5.3134 mL
    15 mM 0.1417 mL 0.7085 mL 1.4169 mL 3.5423 mL
    20 mM 0.1063 mL 0.5313 mL 1.0627 mL 2.6567 mL
    25 mM 0.0850 mL 0.4251 mL 0.8501 mL 2.1254 mL
    30 mM 0.0708 mL 0.3542 mL 0.7085 mL 1.7711 mL
    40 mM 0.0531 mL 0.2657 mL 0.5313 mL 1.3283 mL
    50 mM 0.0425 mL 0.2125 mL 0.4251 mL 1.0627 mL
    60 mM 0.0354 mL 0.1771 mL 0.3542 mL 0.8856 mL
    80 mM 0.0266 mL 0.1328 mL 0.2657 mL 0.6642 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Limonin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Limonin1180-71-8Limonoic acid 3,19:16,17 dilactoneHIVApoptosisEndogenous MetaboliteCytochrome P450P-glycoproteinHuman immunodeficiency virusCYPsP-gpPgpMultidrug resistance protein 1MDR1ATP-binding cassette sub-family B member 1ABCB1Cluster of differentiation 243CD243HIV-1Human cytochrome P450 enzymeSW480Colon cancerInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Limonin
    製品番号:
    HY-17411
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.