2. Apoptosis Immunology/Inflammation MAPK/ERK Pathway NF-κB
  3. Apoptosis Interleukin Related TNF Receptor p38 MAPK NF-κB
  4. Peimine

Peimine  (Synonyms: Verticine; Dihydroisoimperialine)

Art. -Nr.: HY-N0212 Reinheit: 99.9%
COA
SDS
Handling Instructions Technical Support

Peimine (Verticine; Dihydroisoimperialine) is an orally active natural product. Peimine has anti-inflammatory, analgesic and cough relieving effects. Peimine can be used in cancer and inflammation related research.

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Peimine

Peimine Chemische Struktur

CAS. Nr. : 23496-41-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO Auf Lager
Solid
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Based on 4 publication(s) in Google Scholar

Other Forms of Peimine:

Peimine Related Antibodies

Top Publications Citing Use of Products

Alle Interleukin Related Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

Alle TNF Receptor Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

Alle p38 MAPK Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

Alle NF-κB Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biologische Aktivität

  • Reinheit & Dokumentation

  • Verweise

  • Kundenbewertung

Beschreibung

Peimine (Verticine; Dihydroisoimperialine) is an orally active natural product. Peimine has anti-inflammatory, analgesic and cough relieving effects. Peimine can be used in cancer and inflammation related research[1][2][3][4][5][6][7].

IC50 & Target

Ki-67

 

PCNA

 

IL-1

 

IL-6

 

TNF-α

 

IL-1β

 

IL-10

 

p38

 

NFκB

 

In Vitro

Peimine (0-200 μM, 24 h) disrupts intracellular calcium homeostasis through the Ca2+/CaMKII/JNK pathway, inhibits the growth and movement of prostate cancer cells (DU-145, LNCap, and PC-3) and induces apoptosis, while having no significant effect on normal cells (RWPE-1) [3].
Peimine (0-25 mg/L, 1 h) has anti-inflammatory activity through regulating TNF-a, IL-6, IL-1b and IL-10 by suppressing the phosphorylation of MAPK and the activation of NF-kB in LPS-stimulated RAW 264.7 macrophages[6].
Peimine (10-300 μM, 0-10 ms) can block the Nav1.7 ion channel with an IC50 value of 47.2 μM, and can also inhibit the Kv1.3 ion channel with an IC50 value of 354.4 μM[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peimine Related Antibodies

Western Blot Analysis[3]

Cell Line: PC-3 cells
Concentration: 0-10 μM
Incubation Time: 24 h
Result: Decreased the protein expression levels of Ki-67 and PCNA in PC-3 cells in a dose-dependent manner.
In Vivo

Peimine (32.5 μM/kg/day for 7days,i.g.) can effectively alleviate LPS-induced acute lung injury in mice, inhibit the expression levels of inflammatory factors TNF-α, IL-6, and IL-1 in mouse models, and has a synergistic effect when combined with Peiminine (HY-N0213) and Forsythoside. It can effectively improve its anti-inflammatory activity[4].
The drug level of Peimine (single oral 4.25 g/kg) in the blood and tissue of male rats is significantly higher than that of female rats (Reference 3)[5].

Pharmacokinetic Analysis in male and female SD Rats[5]

Gender Route Dose (g/kg) Cmax (ng/mL) Tmax (h) t1/2 (h) MRT0-t (h) MRT0-∞ (h) AUC0-t (ng·h/mL) AUC0-∞ (ng·h/mL) CL/F (L/kg/h) V/F (L/kg)
Female p.o. 4.25 43.7 1.5 4.2 7.8 7.9 214.2 214.3 128.9 781.3
Male p.o. 4.25 57.6 2.9 6.2 7.2 7.4 662.4 665.3 41.5 374.1

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molekulargewicht

431.65

Formel

C27H45NO3
CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

[H][C@]12[C@]3([H])C[C@H](O)[C@@]4([H])C[C@@H](O)CC[C@]4(C)[C@@]3([H])C[C@]([H])1[C@]5([H])CN6C[C@@H](C)CC[C@]([H])6[C@@](C)(O)[C@]([H])5CC2
Structure Classification
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 50 mg/mL (115.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3167 mL 11.5835 mL 23.1669 mL
5 mM 0.4633 mL 2.3167 mL 4.6334 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation

Purity: 99.9%

SDS (393 KB)

COA (258 KB) HNMR (168 KB) CNMR (163 KB) RP-HPLC (176 KB) MS (195 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3167 mL 11.5835 mL 23.1669 mL 57.9173 mL
5 mM 0.4633 mL 2.3167 mL 4.6334 mL 11.5835 mL
10 mM 0.2317 mL 1.1583 mL 2.3167 mL 5.7917 mL
15 mM 0.1544 mL 0.7722 mL 1.5445 mL 3.8612 mL
20 mM 0.1158 mL 0.5792 mL 1.1583 mL 2.8959 mL
25 mM 0.0927 mL 0.4633 mL 0.9267 mL 2.3167 mL
30 mM 0.0772 mL 0.3861 mL 0.7722 mL 1.9306 mL
40 mM 0.0579 mL 0.2896 mL 0.5792 mL 1.4479 mL
50 mM 0.0463 mL 0.2317 mL 0.4633 mL 1.1583 mL
60 mM 0.0386 mL 0.1931 mL 0.3861 mL 0.9653 mL
80 mM 0.0290 mL 0.1448 mL 0.2896 mL 0.7240 mL
100 mM 0.0232 mL 0.1158 mL 0.2317 mL 0.5792 mL
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Peimine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Peimine23496-41-5Verticine DihydroisoimperialineApoptosisInterleukin RelatedTNF Receptorp38 MAPKNF-κBILTumor Necrosis Factor ReceptorTNFRNuclear factor-κBNuclear factor-kappaBprostate cancerintracellular calcium homeostasisapoptosisLPS-induced acute lung injury in miceinflammatoryNav1.7 ion channelKv1.3 ion channelInhibitorinhibitorinhibit

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