1. Autophagy Apoptosis Epigenetics Cell Cycle/DNA Damage MAPK/ERK Pathway Stem Cell/Wnt NF-κB
  2. Autophagy Caspase Bcl-2 Family PARP p38 MAPK ERK NF-κB Apoptosis
  3. Peiminine

Peiminine  (Synonyms: Verticinone; Raddeanine)

Cat. No.: HY-N0213 Purity: 99.94%
COA Handling Instructions

Peiminine is a compound that can be isolated from Bolbostemma paniculatum (Maxim) Franquet (Cucurbitaceae family). Peiminine can induce apoptosis in human hepatocellular carcinoma HepG2 cells through both extrinsic and intrinsic apoptotic pathways. Peiminine has anti-inflammatory, anticancer, anti-osteoporosis, cardioprotective and other activities in many animal models.

For research use only. We do not sell to patients.

Peiminine Chemical Structure

Peiminine Chemical Structure

CAS No. : 18059-10-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 94 In-stock
Solution
10 mM * 1 mL in DMSO USD 94 In-stock
Solid
1 mg USD 40 In-stock
5 mg USD 86 In-stock
10 mg USD 134 In-stock
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Based on 2 publication(s) in Google Scholar

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  • Biological Activity

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Description

Peiminine is a compound that can be isolated from Bolbostemma paniculatum (Maxim) Franquet (Cucurbitaceae family). Peiminine can induce apoptosis in human hepatocellular carcinoma HepG2 cells through both extrinsic and intrinsic apoptotic pathways. Peiminine has anti-inflammatory, anticancer, anti-osteoporosis, cardioprotective and other activities in many animal models[1][2][3][4][5][6].

IC50 & Target

Caspase 3

 

Caspase-8

 

Caspase-9

 

PARP-1

 

ERK1

 

ERK2

 

Bcl-2

 

p65

 

In Vitro

Peiminine (2-6 μg/mL, 24 h) decreases the expressions of procaspase-3, procaspases-8 and -9 and increases the levels of caspase-3, 8, 9 protein in HepG2 cells[1].
Peiminine (2-14 μg/mL, 24-72 h) displays the marked cytotoxicity to HepG2, Hela, SW480 and MCF-7 cells[1].
Peiminine (2-6 μg/mL, 24 h) induces apoptosis in HepG2 cells[1].
Peiminine (2-6 μg/mL, 24 h) induces HepG2 cells arrest at the G2/M phase[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: HepG2, Hela, SW480, MCF-7
Concentration: 2 μg/mL, 4 μg/mL, 6 μg/mL, 8 μg/mL, 10 μg/mL, 12μg/mL, and 14 μg/mL
Incubation Time: 24 h, 48 h, 72 h
Result: Exhibited a significant inhibition on the survival of HepG2, Hela, SW480 and MCF-7 cells with the IC50 values were 4.58, 4.89, 5.07 and 5.12 μg/mL at 24 h, respectively.

Apoptosis Analysis[1]

Cell Line: HepG2
Concentration: 2 μg/mL, 4 μg/mL, 6 μg/mL, 8 μg/mL
Incubation Time: 24 h
Result: Dissociated chromosome to produce DNA fragments dose-dependently.

Cell Cycle Analysis[1]

Cell Line: HepG2
Concentration: 2 μg/mL, 4 μg/mL, 6 μg/mL
Incubation Time: 24 h
Result: Decreased the percentage of G1 phases from 65.15% ± 0.78 to 49.55% ± 0.17 with the increase of concentrations.
Increased the percentage of G2/M phases from 17.32% ± 0.20 to 39.99% ± 0.47 with the increase of concentrations.

Western Blot Analysis[1]

Cell Line: HepG2
Concentration: 2 μg/mL, 4 μg/mL, 6 μg/mL
Incubation Time: 24 h
Result: Reduced the expression of procaspase-3, PARP1, procaspases-8 and -9, and Bcl-2 at 2-6 μg/mL.
Increased caspase-3, 8, 9, PARP1 cleaved and Bax protein levels.
In Vivo

Peiminine (3mg/kg, intraperitoneal injection, single dose) alleviates inflammatory manifestations and mitigates intestinal tissue damage in an experimental model of ulcerative colitis[2].
Peiminine (10 mg/kg, Intraperitoneal injection, once every 2 days for 6 weeks) prevents bone loss and fat formation in OVX-induced rat model[3].
Peiminine (1-5 mg/kg, are applied to the dorsal skin, once daily for 16 days) inhibits serum IL-6 and TNF-α in the dinitrochlorobenzene (DNCB)-induced Allergic dermatitis animal model[4].
Peiminine (2-5 mg/kg, Intraperitoneal injection, once a day for 4 weeks) has a cardioprotective effect against myocardial infarction-induced myocardial injury and fibrosis in myocardial infarction rat model[5].
Peiminine (1-5 mg/kg, Intraperitoneal injection, single dose) can reduce the damage of inflammatory response to the body and the possibility of pulmonary edema in LPS-induced Acute lung injury model mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ulcerative colitis model[2]
Dosage: 3mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Reduced inflammation, mucosal ulcers, involvement of digestive system layers, and infltration of inflammatory cells.
Reduced the levels of MPO and NO generated in the rectal tissue.
Reduced cell proliferation in spleen cell.
Decreased the production of f IL-1β, IL-6, and TNF-α cytokines.
Reduced expression levels of genes IL-1β, IL-6, TNF-α, iNOS, and COX2.
Animal Model: Ovariectomized (OVX) rat model[3]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Alleviated the bone loss caused by surgical castration.
Improved the expression of COL1A1 and β-catenin and reduced the increase expression of PPAR-γ in trabecular bone.
Animal Model: Allergic dermatitis model[4]
Dosage: 1 mg/kg, 5 mg/kg
Administration: were applied to the dorsal skin
Result: Alleviated the bone loss caused by surgical castration.
Improved the expression of COL1A1 and β-catenin and reduced the increase expression of PPAR-γ in trabecular bone.
Molecular Weight

429.64

Formula

C27H43NO3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[H][C@]12[C@]3([H])CC([C@@]4([H])C[C@@H](O)CC[C@]4(C)[C@@]3([H])C[C@]([H])1[C@]5([H])CN6C[C@@H](C)CC[C@]([H])6[C@@](C)(O)[C@]([H])5CC2)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (232.75 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3275 mL 11.6376 mL 23.2753 mL
5 mM 0.4655 mL 2.3275 mL 4.6551 mL
10 mM 0.2328 mL 1.1638 mL 2.3275 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.94%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Peiminine
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