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Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione), an alkaloid isolated from green algae Ulva prolifera, possesses antialgal activity against the common harmful red tide microalgae . Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione) possesses antiamnesic effects and neuroprotective actions .
Dimedone (5,5-Dimethylcyclohexane-1,3-dione) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Hexane-2,5-dione (2,5-HD) is an orally active, CNS-penetrant cytotoxic agent . Hexane-2,5-dione reduces BCL-2 and β-catenin/TCF transcriptional activity, increases BAX and active caspase-3 expression, and promotes apoptosis. Hexane-2,5-dione causes an accumulation of neurofilaments within axons in rats. Hexane-2,5-dione can be used for the research of neurodegenerative diseases .
Cyclohexane-1,3-dione is a drug intermediate, and its derivatives can exhibit significant tyrosine kinase inhibitory activity and anti-tumor activity .
1,4-Dioxacycloheptadecane-5,17-dione is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
(25R)-Spirost-4-ene-3,12-dione ((-)-(25R)-Spirost-4-ene-3,12-dione) is a natural product that has an inhibitory effect on neutrophil superoxide anion production and histamine release from mast cells .
6,7-Dichloroquinoline-5,8-dione (compound DA3002) is a potent CDC25B2 inhibitor with an IC50 value of 4.6 µM. 6,7-Dichloroquinoline-5,8-dione has the potential for the research of cancer .
3-Bromo-1H-pyrrole-2,5-dione (compound 2c) is a 3-substituted pyrrole-2,5-dione compound with antibacterial activity. 3-Bromo-1H-pyrrole-2,5-dione inhibits pathogenic strains of S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853, with MIC values of 32 μg/mL, 32 μg/mL, and 64 μg/mL, respectively .
(5α)-Stigmastane-3,6-dione is a naturally occurring sterol that could be isolated from fruits of Ailanthus altissima Swingle. Antimicrobial Activity. .
(R)-2-(2-Hydroxy-3-((4-(3-oxomorpholino)phenyl)amino)propyl)isoindoline-1,3-dione (1H-Isoindole-1,3(2H)-dione,2-[(2R)-2-hydroxy-3-[[4-(3-oxo-4-morpholinyl)phenyl]amino]propyl) is a drug intermediate for synthesis of various active compounds.
Muurol-4-ene-3,8-dione (Compound 2), a sesquiterpene, is one of the main hepatotoxic components in Eupatorium adenophorum. Muurol-4-ene-3,8-dione has an inhibitory effect on the growth of both the normal human hepatocyte cell L02 and the human hepatocellular carcinoma cell HepG2 with IC50 values of 87.52 and 104.48 μM .
Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione), an alkaloid isolated from green algae Ulva prolifera, possesses antialgal activity against the common harmful red tide microalgae . Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione) possesses antiamnesic effects and neuroprotective actions .
5β-Cholanic acid-3,6-dione (3,6-Diketo-LCA) is a similar substance to Lithocholic acid (LCA) (HY-B0172). 5β-Cholanic acid-3,6-dione cannot activate the Vitamin D receptor (VDR), and can be used as a negative control for studying the relationship between LCA derivatives and VDR .
Cycloheptane-1,3-dione (Compound 1A) is an intermediate. Cycloheptane-1,3-dione can be used to synthesize Compound 1-1. Compound 1-1 has anticancer activity against colon cancer .
5-(tert-Butyl)isobenzofuran-1,3-dione is a intermediate and dye. 5-(tert-Butyl)isobenzofuran-1,3-dione has a synonym of 4-tert-Butylphthalic Anhydride .
1-Benzylpyrrole-2,5-dione (N-Benzylmaleimide) is a heterocyclic compound containing a maleimide ring and a benzyl group. 1-Benzylpyrrole-2,5-dione can be used as a key reactant for the green chemical modification of cellulose nanocrystals (CNC).
Androsta-1,4,6-triene-3,17-dione is a lipophilic and specific aromatase inhibitor with a Ki of 0.18 μM. Androsta-1,4,6-triene-3,17-dione inhibits estrogen biosynthesis and shows antifertility effects. Androsta-1,4,6-triene-3,17-dione induces impairment of spatial memory .
3a,4,7,7a-Tetrahydro-1H-isoindole-1,3(2H)-dione-d6 (1,2,3,6-Tetrahydrophthalimide-d6) is the deuterium labeled 3a,4,7,7a-Tetrahydro-1H-isoindole-1,3(2H)-dione (HY-W068988).
Cyclohexane-1,3-dione- 13C6 is the 13C-labeled Cyclohexane-1,3-dione (HY-W007466). Cyclohexane-1,3-dione is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Cyclohexane-1,3-dione- 13C3 is the 13C-labeled Cyclohexane-1,3-dione (HY-W007466). Cyclohexane-1,3-dione is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
2-(Isopentylamino)naphthalene-1,4-dione (compound 3d), a Vitamin K (HY-B2172) analogue, shows protection in Pentylenetetrazole (PTZ)-induced seizure model. 2-(Isopentylamino)naphthalene-1,4-dione significantly increases ATP levels in zebrafish as well as HT-22 cells. 2-(Isopentylamino)naphthalene-1,4-dione shows excellent permeability into the brain .
Olean-12-ene-3,11-dione is a natural product that can be isolated from Euonymus laxiflorus and Canarium zeylanicum. Olean-12-ene-3,11-dione has antiinflammatory activity .
2-(3-azidopropyl)-isoindole-1,3-dione is a phthalimide with a terminal azide group connected by a 3-carbon linker. The azide group enables Click Chemistry.
3-Tosylimidazolidine-2,4-dione (compound 2C) is a hydantoins derivative, and exhibits inhibitory activity against the Pf3D7 strain, with an IC50 value of 3.97 nM .
ent-11β-Hydroxyatis-16-ene-3,14-dione (compound 11) is a diterpenoid from the fresh roots of Euphorbia jolkinii. ent-11β-Hydroxyatis-16-ene-3,14-dione has anti-RSV activity .
5β-Cholanic acid 3,7-dione methyl ester is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
4-Methylhexahydroisobenzofuran-1,3-dione (3-Methylhexahydrophthalic anhydride) is mainly used as an intermediate for solvents, resins and adhesives in industry.
Benz[g]isoquinoline-5,10-dione, an active component isolated from the ethanolic extract of the aerial parts of Mitracarpus scaber, demonstrates notable in vitro inhibitory activity against AIDS-related pathogens, along with significant antibacterial and antifungal properties, as evidenced by the agar well-diffusion assay.
2-(7-Octyn-1-yl)-1H-isoindole-1,3-dione is a phthalimide with a terminal propargyl group connected by a 6-carbon linker. The alkyne can participate in copper-catalyzed Click Chemistry.
5-Hydroxy-2-(1-methyl-2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione is a Thalidomide analogue featuring a hydroxy group, which is a versatile site for further derivitization. Thalidomide is an E3 ligase activator and promotes the ubiquitinylation of a given protein.
(R)-2-(2-Hydroxy-3-((4-(3-oxomorpholino)phenyl)amino)propyl)isoindoline-1,3-dione (Standard) is the analytical standard of (R)-2-(2-Hydroxy-3-((4-(3-oxomorpholino)phenyl)amino)propyl)isoindoline-1,3-dione. This product is intended for research and analytical applications.
2-Methylcyclopentane-1,3-dione (Standard) is the analytical standard of 2-Methylcyclopentane-1,3-dione (HY-W012944). This product is intended for research and analytical applications. 2-Methylcyclopentane-1,3-dione is a key intermediate for the total synthesis of steroids .
Cyclopent-4-ene-1,3-dione is a potent antifungal inhibitor of chitin synthesis. Cyclopent-4-ene-1,3-dione is potently antifungal against human pathogenic Candida species (IC50 = 1-2 μM). Cyclopent-4-ene-1,3-dione is detected in feijoa cultivars with high antifungal bioactivity. Cyclopent-4-ene-1,3-dione can be used for the research of fungal infections .
1,4-Dioxane-2,5-dione (Standard) is the analytical standard of 1,4-Dioxane-2,5-dione. This product is intended for research and analytical applications.
2-Methylisoindoline-1,3-dione (Standard) is the analytical standard of 2-Methylisoindoline-1,3-dione. This product is intended for research and analytical applications.
8-Azaspiro[4.5]decane-7,9-dione (Standard) is the analytical standard of 8-Azaspiro[4.5]decane-7,9-dione. This product is intended for research and analytical applications.
1-(2,4,5-Trimethoxyphenyl)propane-1,2-dione is an aromatic compound found in Acorus calamus. 1-(2,4,5-Trimethoxyphenyl)propane-1,2-dione is promising for research of plant allelopathy and weed control .
1-Ethylpiperazine-2,3-dione (Standard) is the analytical standard of 1-Ethylpiperazine-2,3-dione. This product is intended for research and analytical applications.
1,4,5,8-Tetrakis((4-chlorophenyl)thio)anthracene-9,10-dione (1,4,5,8-Tetrakis[(4-chlorophenyl)sulfanyl]anthracene-9,10-dione) is a metal-organic framework (MOF).
2-(4-Azidobutyl)isoindoline-1,3-dione is a phthalimide with a terminal azide group connected by a 4-carbon linker. The azide group enables Click Chemistry.
2-(6-Azidohexyl)isoindoline-1,3-dione is a phthalimide with a terminal azide group connected by a 6-carbon linker. The azide group enables Click Chemistry.
2-(5-Azidopentyl)isoindoline-1,3-dione is a phthalimide with a terminal azide group connected by a 5-carbon linker. The azide group enables Click Chemistry.
6,7-Dihydropyrazino[2,3-d]pyridazine-5,8-dione (Impurity) (Standard) is the analytical standard of 6,7-Dihydropyrazino[2,3-d]pyridazine-5,8-dione (Impurity). This product is intended for research and analytical applications.
2,6-Di-tert-butylcyclohexa-2,5-diene-1,4-dione (Standard) is the analytical standard of 2,6-Di-tert-butylcyclohexa-2,5-diene-1,4-dione. This product is intended for research and analytical applications.
2-Methyl-3-thioxo-1,2,4-triazinane-5,6-dione (Standard) is the analytical standard of 2-Methyl-3-thioxo-1,2,4-triazinane-5,6-dione. This product is intended for research and analytical applications.
3,3'-(1,3,5,7-Tetraoxopyrrolo[3,4-f]isoindole-2,6(1H,3H,5H,7H)-diyl)dibenzoic acid (3,3'-(1,3,5,7-Tetraoxo-5,7-dihydropyrrolo[3,4-f]isoindole-2,6(1H,3H)-diyl)dibenzoic acid) is a metal-organic framework (MOF).
5-((1H-Indol-3-yl)methylene)imidazolidine-2,4-dione is an inhibitor of dengue virus NS2B-NS3 protease and thrombin. 5-((1H-Indol-3-yl)methylene)imidazolidine-2,4-dione can be used in the research of infectious diseases .
5-(tert-Butyl)isobenzofuran-1,3-dione is a intermediate and dye. 5-(tert-Butyl)isobenzofuran-1,3-dione has a synonym of 4-tert-Butylphthalic Anhydride .
Dimedone (5,5-Dimethylcyclohexane-1,3-dione) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Cyclohexane-1,3-dione is a drug intermediate, and its derivatives can exhibit significant tyrosine kinase inhibitory activity and anti-tumor activity .
1,4-Dioxacycloheptadecane-5,17-dione is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Cycloheptane-1,3-dione (Compound 1A) is an intermediate. Cycloheptane-1,3-dione can be used to synthesize Compound 1-1. Compound 1-1 has anticancer activity against colon cancer .
1-Benzylpyrrole-2,5-dione (N-Benzylmaleimide) is a heterocyclic compound containing a maleimide ring and a benzyl group. 1-Benzylpyrrole-2,5-dione can be used as a key reactant for the green chemical modification of cellulose nanocrystals (CNC).
5β-Cholanic acid 3,7-dione methyl ester is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione), an alkaloid isolated from green algae Ulva prolifera, possesses antialgal activity against the common harmful red tide microalgae . Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione) possesses antiamnesic effects and neuroprotective actions .
(25R)-Spirost-4-ene-3,12-dione ((-)-(25R)-Spirost-4-ene-3,12-dione) is a natural product that has an inhibitory effect on neutrophil superoxide anion production and histamine release from mast cells .
(5α)-Stigmastane-3,6-dione is a naturally occurring sterol that could be isolated from fruits of Ailanthus altissima Swingle. Antimicrobial Activity. .
Muurol-4-ene-3,8-dione (Compound 2), a sesquiterpene, is one of the main hepatotoxic components in Eupatorium adenophorum. Muurol-4-ene-3,8-dione has an inhibitory effect on the growth of both the normal human hepatocyte cell L02 and the human hepatocellular carcinoma cell HepG2 with IC50 values of 87.52 and 104.48 μM .
Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione), an alkaloid isolated from green algae Ulva prolifera, possesses antialgal activity against the common harmful red tide microalgae . Cyclo-(Pro-Gly) (Pyrrolopiperazine-2,5-dione) possesses antiamnesic effects and neuroprotective actions .
Olean-12-ene-3,11-dione is a natural product that can be isolated from Euonymus laxiflorus and Canarium zeylanicum. Olean-12-ene-3,11-dione has antiinflammatory activity .
ent-11β-Hydroxyatis-16-ene-3,14-dione (compound 11) is a diterpenoid from the fresh roots of Euphorbia jolkinii. ent-11β-Hydroxyatis-16-ene-3,14-dione has anti-RSV activity .
2-Methylcyclopentane-1,3-dione (Standard) is the analytical standard of 2-Methylcyclopentane-1,3-dione (HY-W012944). This product is intended for research and analytical applications. 2-Methylcyclopentane-1,3-dione is a key intermediate for the total synthesis of steroids .
Cyclopent-4-ene-1,3-dione is a potent antifungal inhibitor of chitin synthesis. Cyclopent-4-ene-1,3-dione is potently antifungal against human pathogenic Candida species (IC50 = 1-2 μM). Cyclopent-4-ene-1,3-dione is detected in feijoa cultivars with high antifungal bioactivity. Cyclopent-4-ene-1,3-dione can be used for the research of fungal infections .
1-(2,4,5-Trimethoxyphenyl)propane-1,2-dione is an aromatic compound found in Acorus calamus. 1-(2,4,5-Trimethoxyphenyl)propane-1,2-dione is promising for research of plant allelopathy and weed control .
The kshB protein significantly degrades cholesterol by catalyzing the introduction of the 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD), resulting in the 9OHADD intermediate. It is spontaneously converted to HSA through the cleavage of the B ring and aromatization of the A ring. kshB Protein, Mycobacterium tuberculosis is the recombinant kshB protein, expressed by E. coli , with tag free.
The kshB protein significantly degrades cholesterol by catalyzing the introduction of the 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD), resulting in the 9OHADD intermediate. It is spontaneously converted to HSA through the cleavage of the B ring and aromatization of the A ring. kshB Protein, Mycobacterium tuberculosis (His) is the recombinant kshB protein, expressed by E. coli , with N-6*His labeled tag.
3a,4,7,7a-Tetrahydro-1H-isoindole-1,3(2H)-dione-d6 (1,2,3,6-Tetrahydrophthalimide-d6) is the deuterium labeled 3a,4,7,7a-Tetrahydro-1H-isoindole-1,3(2H)-dione (HY-W068988).
Cyclohexane-1,3-dione- 13C6 is the 13C-labeled Cyclohexane-1,3-dione (HY-W007466). Cyclohexane-1,3-dione is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Cyclohexane-1,3-dione- 13C3 is the 13C-labeled Cyclohexane-1,3-dione (HY-W007466). Cyclohexane-1,3-dione is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
MedchemExpress Validation 03
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
MedchemExpress Validation 04
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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