5a-Pregnane-3,20-dione

Name: 5a-Pregnane-3,20-dione
Brand: MedChemExpress
SKU: HY-W006492
Rating: 4.5 (1 reviews)

Cat. No.: HY-W006492 Purity: 99.29%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5a-Pregnane-3,20-dione is the endogenous progesterone metabolite.

For research use only. We do not sell to patients.

5a-Pregnane-3,20-dione Chemical Structure

CAS No. : 566-65-4

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100 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
1 g	USD 105	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5a-Pregnane-3,20-dione:

5a-Pregnane-3,20-dione-d₆ In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5a-Pregnane-3,20-dione is the endogenous progesterone metabolite.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

5a-Pregnane-3,20-dione decreases cell-substrate attachment, adhesion plaques, vinculin expression, and polymerizes F-actin in MCF-7 breast cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

316.48

Formula

C₂₁H₃₂O₂

CAS No.

566-65-4

Appearance

Solid

Color

White to off-white

SMILES

CC([C@H]1CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])CC(CC[C@]4(C)[C@@]3([H])CC[C@]12C)=O)=O

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 6.67 mg/mL (21.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1598 mL	15.7988 mL	31.5976 mL
5 mM	0.6320 mL	3.1598 mL	6.3195 mL
10 mM	0.3160 mL	1.5799 mL	3.1598 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.67 mg/mL (2.12 mM); Clear solution

This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.29%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (252 KB) HNMR (268 KB) RP-HPLC (148 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Wiebe JP, et al. The endogenous progesterone metabolite, 5a-pregnane-3,20-dione, decreases cell-substrate attachment, adhesion plaques, vinculin expression, and polymerized F-actin in MCF-7 breast cancer cells. Endocrine. 2001 Oct;16(1):7-14. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1598 mL	15.7988 mL	31.5976 mL	78.9939 mL
	5 mM	0.6320 mL	3.1598 mL	6.3195 mL	15.7988 mL
	10 mM	0.3160 mL	1.5799 mL	3.1598 mL	7.8994 mL
	15 mM	0.2107 mL	1.0533 mL	2.1065 mL	5.2663 mL
	20 mM	0.1580 mL	0.7899 mL	1.5799 mL	3.9497 mL