1. GPCR/G Protein
    Autophagy
    Metabolic Enzyme/Protease
  2. Cannabinoid Receptor
    Autophagy
    Endogenous Metabolite

Pregnenolone (Synonyms: Arthenolone; 3β-Hydroxy-5-pregnen-20-one)

Cat. No.: HY-B0151 Purity: >98.0%
Handling Instructions

Pregnenolone acts as a signaling-specific inhibitor of cannabinoid CB1 receptor, reduces several effects of tetrahydrocannabinol (THC).

For research use only. We do not sell to patients.

Pregnenolone Chemical Structure

Pregnenolone Chemical Structure

CAS No. : 145-13-1

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 96 In-stock
Estimated Time of Arrival: December 31
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Other Forms of Pregnenolone:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Pregnenolone acts as a signaling-specific inhibitor of cannabinoid CB1 receptor, reduces several effects of tetrahydrocannabinol (THC).

IC50 & Target[1]

CB1 Receptor

 

Human Endogenous Metabolite

 

In Vitro

The effect of THC is significantly attenuated when slices are pre-treated with Pregnenolone 100 nM (15.1±1.8 % of inhibition). These effects are likely due to a pre-synaptic action of Pregnenolone. Thus, Pregnenolone blocks the increase in paired-pulse ratio (PPR) induced by THC but does not modify either the amplitude or the decay time of miniature EPSC (mEPSC)[1].

In Vivo

Pregnenolone administration (2-6 mg/kg) blocks THC-induced food-intake in Wistar rats and in C57BL/6N mice, and blunts the memory impairment induced by THC in mice, but it does not modify these behaviors per se. Injections of Pregnenolone (2 and 4mg/kg) before each self-administration session reduce the intake of WIN 55,212-2 and reduce the break-point in a progressive ratio schedule[1].

Clinical Trial
Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1598 mL 15.7988 mL 31.5976 mL
5 mM 0.6320 mL 3.1598 mL 6.3195 mL
10 mM 0.3160 mL 1.5799 mL 3.1598 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[1]

Mice and Rats[1]
Adult male Wistar rats (weighing 320-340g), Sprague Dawley male rats (weighing 330-350g), C57BL/6N mice (2-3 months) and CD1 mice (weighing 25-30 g at the beginning of the experiments) are used. Pregnenolone is injected subcutaneously (sc). The injection volumes are 1 mL/kg of body weight for rats and 10 mL/kg for mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

316.48

Formula

C₂₁H₃₂O₂

CAS No.

145-13-1

SMILES

C[[email protected]@](C1=CC2)(CC[C[email protected]](O)C1)[[email protected]]3([H])[[email protected]]2([H])[[email protected]@](CC[[email protected]@H]4C(C)=O)([H])[[email protected]]4(C)CC3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Pregnenolone
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HY-B0151
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Pregnenolone

Cat. No.: HY-B0151