1. Metabolic Enzyme/Protease
    GPCR/G Protein
  2. Endogenous Metabolite
    Cannabinoid Receptor

Pregnenolone monosulfate (Synonyms: Pregn-5-en-20-on-3β-yl sulfuric acid)

Cat. No.: HY-B1739
Handling Instructions

Pregnenolone monosulfate acts as a signaling-specific inhibitor of cannabinoid CB1 receptor, reduces several effects of tetrahydrocannabinol (THC).

For research use only. We do not sell to patients.

Pregnenolone monosulfate Chemical Structure

Pregnenolone monosulfate Chemical Structure

CAS No. : 1247-64-9

Size Price Stock
50 mg USD 50 Get quote

* Please select Quantity before adding items.

Other In-stock Forms of Pregnenolone monosulfate:

Other Forms of Pregnenolone monosulfate:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Pregnenolone monosulfate acts as a signaling-specific inhibitor of cannabinoid CB1 receptor, reduces several effects of tetrahydrocannabinol (THC).

IC50 & Target[1]

CB1 Receptor


Human Endogenous Metabolite


In Vitro

The effect of THC is significantly attenuated when slices are pre-treated with Pregnenolone 100 nM (15.1±1.8 % of inhibition). These effects are likely due to a pre-synaptic action of Pregnenolone. Thus, Pregnenolone blocks the increase in paired-pulse ratio (PPR) induced by THC but does not modify either the amplitude or the decay time of miniature EPSC (mEPSC)[1].

In Vivo

Pregnenolone administration (2-6 mg/kg) blocks THC-induced food-intake in Wistar rats and in C57BL/6N mice, and blunts the memory impairment induced by THC in mice, but it does not modify these behaviors per se. Injections of Pregnenolone (2 and 4mg/kg) before each self-administration session reduce the intake of WIN 55,212-2 and reduce the break-point in a progressive ratio schedule[1].

Clinical Trial
Solvent & Solubility
In Vitro: 

10 mM in DMSO

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5218 mL 12.6091 mL 25.2181 mL
5 mM 0.5044 mL 2.5218 mL 5.0436 mL
10 mM 0.2522 mL 1.2609 mL 2.5218 mL
*Please refer to the solubility information to select the appropriate solvent.
Animal Administration

Mice and Rats[1]
Adult male Wistar rats (weighing 320-340g), Sprague Dawley male rats (weighing 330-350g), C57BL/6N mice (2-3 months) and CD1 mice (weighing 25-30 g at the beginning of the experiments) are used. Pregnenolone is injected subcutaneously (sc). The injection volumes are 1 mL/kg of body weight for rats and 10 mL/kg for mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







CC([[email protected]]1CC[[email protected]@]2([H])[[email protected]]3([H])CC=C4C[[email protected]@H](OS(=O)(O)=O)CC[[email protected]]4(C)[[email protected]@]3([H])CC[[email protected]]12C)=O


Please store the product under the recommended conditions in the Certificate of Analysis.


Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name



Applicant name *


Email address *

Phone number *


Organization name *

Country or Region *


Requested quantity *


Bulk Inquiry

Inquiry Information

Product Name:
Pregnenolone monosulfate
Cat. No.:

Pregnenolone monosulfate

Cat. No.: HY-B1739